##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632573.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1061085
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.07630962646725	34.0	33.0	34.0	31.0	34.0
2	33.2106749223672	34.0	33.0	34.0	31.0	34.0
3	33.28124231329252	34.0	34.0	34.0	31.0	34.0
4	36.55347592322953	37.0	37.0	37.0	35.0	37.0
5	36.527215067595904	37.0	37.0	37.0	35.0	37.0
6	36.57306247850078	37.0	37.0	37.0	35.0	37.0
7	36.57332918663444	37.0	37.0	37.0	35.0	37.0
8	36.547015554833024	37.0	37.0	37.0	35.0	37.0
9	38.340558956162795	39.0	39.0	39.0	37.0	39.0
10	38.37632611901968	39.0	39.0	39.0	37.0	39.0
11	38.445522272014024	39.0	39.0	39.0	37.0	39.0
12	38.40707671864177	39.0	39.0	39.0	37.0	39.0
13	38.43547406663934	39.0	39.0	39.0	37.0	39.0
14	39.98733089243557	41.0	40.0	41.0	38.0	41.0
15	39.99941003783863	41.0	40.0	41.0	38.0	41.0
16	39.953948081444935	41.0	40.0	41.0	38.0	41.0
17	39.95443060640759	41.0	40.0	41.0	38.0	41.0
18	39.955374922838416	41.0	40.0	41.0	38.0	41.0
19	39.95872338219841	41.0	40.0	41.0	38.0	41.0
20	39.93863168360687	41.0	40.0	41.0	38.0	41.0
21	39.90067148249198	41.0	40.0	41.0	38.0	41.0
22	39.8716851147646	41.0	40.0	41.0	38.0	41.0
23	39.83734950545904	41.0	40.0	41.0	38.0	41.0
24	39.82077213418341	41.0	40.0	41.0	38.0	41.0
25	39.78077628088231	41.0	40.0	41.0	38.0	41.0
26	39.68948576221509	41.0	40.0	41.0	37.0	41.0
27	39.598737141699296	41.0	40.0	41.0	37.0	41.0
28	39.55819467808894	41.0	40.0	41.0	37.0	41.0
29	39.520457833255584	41.0	40.0	41.0	37.0	41.0
30	39.47825197792825	41.0	40.0	41.0	37.0	41.0
31	39.419630849554935	41.0	39.0	41.0	36.0	41.0
32	39.37073090280232	41.0	39.0	41.0	36.0	41.0
33	39.30136888185206	41.0	39.0	41.0	36.0	41.0
34	39.26789748229407	41.0	39.0	41.0	36.0	41.0
35	39.18501910780004	41.0	39.0	41.0	35.0	41.0
36	39.13493358213527	41.0	39.0	41.0	35.0	41.0
37	39.07316190503117	41.0	39.0	41.0	35.0	41.0
38	39.00185470532521	41.0	39.0	41.0	35.0	41.0
39	38.91143499342654	40.0	39.0	41.0	35.0	41.0
40	38.838096853692214	40.0	38.0	41.0	35.0	41.0
41	38.78368839442646	40.0	38.0	41.0	35.0	41.0
42	38.71538001196888	40.0	38.0	41.0	35.0	41.0
43	37.97934566976255	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	2.0
15	1.0
16	2.0
17	5.0
18	24.0
19	26.0
20	72.0
21	100.0
22	200.0
23	380.0
24	598.0
25	854.0
26	1213.0
27	1658.0
28	2278.0
29	3233.0
30	4270.0
31	5816.0
32	7630.0
33	10385.0
34	16109.0
35	25210.0
36	39667.0
37	76273.0
38	202774.0
39	662304.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.82493862414415	16.46380827172187	12.636310945871443	28.074942158262534
2	21.41185673155308	19.617467026675524	31.961341457093447	27.009334784677947
3	21.480936965464593	19.574303660875426	28.805892082161183	30.138867291498794
4	15.964036811377033	15.742848122440709	33.910478425385335	34.38263664079692
5	17.519897086472806	32.728857725818386	32.46318626688719	17.288058920821612
6	34.038177902807035	33.64367604857292	15.443626099699836	16.87451994892021
7	30.011262057233868	27.484320294792596	19.594094723796868	22.91032292417667
8	26.99303071855695	30.666157753620116	20.417308698172153	21.923502829650783
9	27.919723679064358	12.940622099077832	17.508964880287632	41.63068934157019
10	19.52218719518229	23.173638304188636	29.346942045170742	27.957232455458332
11	39.64715362105769	19.37384846642823	18.739120805590503	22.239877106923572
12	22.972806137114368	23.497175061375856	26.49523836450426	27.03478043700552
13	35.29396796675102	17.486911981603736	20.725012605022215	26.494107446623033
14	24.20861665182337	19.99773816423755	22.75661233548679	33.03703284845229
15	29.887897765023535	24.198061418265265	19.399576848226108	26.514463968485085
16	25.69662185404562	23.825895192185357	22.15298491638276	28.32449803738626
17	27.095661516278145	23.437896115768293	21.55623724772285	27.910205120230707
18	26.541040538693885	21.115838976142346	23.396994585730642	28.946125899433127
19	28.337880565647428	22.299533025158212	23.005131539886058	26.3574548693083
20	29.850106259159258	20.77948514963457	22.536083348647846	26.834325242558325
21	27.737363170716765	22.053464142834926	21.60420701451816	28.60496567193015
22	28.2474071351494	22.811461852726218	21.00114505435474	27.939985957769643
23	28.213573841869405	21.86497782929737	21.95526277348186	27.966185555351363
24	28.173614743399444	21.528812489103135	22.226777308132714	28.070795459364707
25	28.52664960865529	22.083339223530633	22.40687598071785	26.983135187096224
26	28.614389987607026	22.496312736491422	21.841228553791638	27.048068722109914
27	27.29621095388211	21.760933384224636	23.891017213512583	27.051838448380668
28	26.484683130946152	23.070347804370055	21.763289463143856	28.681679601539933
29	27.19141256355523	23.537322646159357	21.93528322424688	27.335981566038537
30	26.816136313301953	22.479066238802737	23.513950343280698	27.19084710461462
31	28.263899687583937	22.169477468817295	22.25938544037471	27.30723740322406
32	25.454134211679552	22.051579279699553	22.674243816470877	29.820042692150018
33	26.22155623724772	21.65604075074099	24.058581546247474	28.063821465763816
34	26.270845408237793	21.68836615351268	23.68613259069726	28.35465584755227
35	26.257180150506322	23.084390034728603	24.000527761677905	26.657902053087167
36	26.270279949297183	21.839343690656264	24.7542845295146	27.136091830531957
37	26.941102739177353	21.157777180904453	24.237737787264923	27.663382292653278
38	25.764948142702988	20.714928587247957	25.834028376614505	27.68609489343455
39	25.076878855134133	19.652336994679974	26.99632922904386	28.274454921142038
40	24.809887992008182	20.803328668297073	27.91680214120452	26.469981198490224
41	23.301808997394176	19.942323188057507	28.90805166409854	27.847816150449777
42	21.948665752508045	20.59816131601144	29.838702837190233	27.614470094290276
43	21.023197953038636	21.290377302478124	29.242991843254785	28.443432901228462
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	5.0
2	7.0
3	18.5
4	30.0
5	30.0
6	34.0
7	38.0
8	45.0
9	52.0
10	71.5
11	91.0
12	91.0
13	212.0
14	333.0
15	559.5
16	786.0
17	814.5
18	843.0
19	843.0
20	1042.0
21	1241.0
22	1213.0
23	1185.0
24	1416.0
25	1647.0
26	1647.0
27	2121.5
28	2596.0
29	3747.5
30	4899.0
31	5854.0
32	6809.0
33	6809.0
34	8656.5
35	10504.0
36	12022.5
37	13541.0
38	18254.0
39	22967.0
40	22967.0
41	27210.0
42	31453.0
43	36645.0
44	41837.0
45	57823.0
46	73809.0
47	73809.0
48	84681.5
49	95554.0
50	107927.5
51	120301.0
52	125841.0
53	131381.0
54	131381.0
55	121290.0
56	111199.0
57	106806.0
58	102413.0
59	93910.0
60	85407.0
61	85407.0
62	76261.5
63	67116.0
64	55094.0
65	43072.0
66	37121.5
67	31171.0
68	31171.0
69	26483.0
70	21795.0
71	18648.5
72	15502.0
73	12134.0
74	8766.0
75	8766.0
76	7018.5
77	5271.0
78	4225.5
79	3180.0
80	2515.0
81	1850.0
82	1850.0
83	1488.0
84	1126.0
85	919.5
86	713.0
87	520.0
88	327.0
89	327.0
90	253.0
91	179.0
92	118.5
93	58.0
94	42.5
95	27.0
96	27.0
97	16.0
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1061085.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.14185480943184
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.36201874164986	34.70809926499527
2	7.69476011733773	6.3315340708183125
3	2.283091932228123	2.81791910376944
4	1.1827610129171828	1.9464392747078112
5	0.7354800585902765	1.5129506892876792
6	0.519619318532105	1.2826861535534269
7	0.368339691584585	1.060792467820673
8	0.2928869085894221	0.9639928535015474
9	0.24435958728740698	0.9048065995324067
>10	1.8109620421459667	15.361489913118476
>50	0.2803936723777881	8.106028424331345
>100	0.20182656348132522	16.408550109354056
>500	0.017049275907714555	4.838600908165137
>1k	0.006451077370486589	3.756110167044465
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2944	0.27745185352728574	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2373	0.22363901101231284	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1794	0.16907222324318974	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1741	0.16407733593444446	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1738	0.16379460646413813	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1692	0.15945942125277426	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1629	0.1535221023763412	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1585	0.1493754034785149	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1577	0.14862145822436468	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1426	0.13439074155227904	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1360	0.12817069320553962	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	1339	0.12619158691339524	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	1325	0.12487218271863235	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1298	0.12232761748587531	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1255	0.1182751617448178	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	1240	0.11686151439328611	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	1227	0.11563635335529199	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1221	0.11507089441467931	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1191	0.11224359971161593	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	1170	0.11026449341947157	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1120	0.10555233558103261	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	1117	0.10526960611072628	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	1089	0.10263079772120046	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1074	0.10121715036966877	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	9.424315676877912E-5	0.0	0.0	0.0	0.0
6	9.424315676877912E-5	0.0	0.0	0.0	0.0
7	9.424315676877912E-5	0.0	0.0	0.0	0.0
8	9.424315676877912E-5	0.0	0.0	0.0	0.0
9	9.424315676877912E-5	0.0	0.0	0.0	0.0
10	1.8848631353755825E-4	0.0	0.0	0.0	0.0
11	1.8848631353755825E-4	0.0	0.0	0.0	0.0
12	2.827294703063374E-4	0.0	0.0	0.0	0.0
13	2.827294703063374E-4	0.0	0.0	9.424315676877912E-5	0.0
14	3.769726270751165E-4	0.0	0.0	1.8848631353755825E-4	0.0
15	3.769726270751165E-4	0.0	0.0	1.8848631353755825E-4	0.0
16	3.769726270751165E-4	0.0	0.0	4.7121578384389564E-4	0.0
17	3.769726270751165E-4	0.0	0.0	8.481884109190122E-4	0.0
18	3.769726270751165E-4	0.0	0.0	9.424315676877913E-4	0.0
19	3.769726270751165E-4	0.0	0.0	0.001507890508300466	0.0
20	4.7121578384389564E-4	0.0	0.0	0.0019791062921443617	0.0
21	4.7121578384389564E-4	0.0	0.0	0.0033927536436760487	0.0
22	4.7121578384389564E-4	0.0	0.0	0.006125805189970643	0.0
23	4.7121578384389564E-4	0.0	0.0	0.007256723071195993	0.0
24	4.7121578384389564E-4	0.0	0.0	0.009895531460721809	0.0
25	4.7121578384389564E-4	0.0	0.0	0.011591908282559833	0.0
26	4.7121578384389564E-4	0.0	0.0	0.01611557980746123	0.0
27	4.7121578384389564E-4	0.0	0.0	0.03732029008043653	9.424315676877912E-5
28	4.7121578384389564E-4	0.0	0.0	0.13853744045010533	9.424315676877912E-5
29	4.7121578384389564E-4	0.0	0.0	0.2876301144583139	9.424315676877912E-5
30	4.7121578384389564E-4	0.0	0.0	0.4967556793282348	9.424315676877912E-5
31	4.7121578384389564E-4	0.0	0.0	1.0776704976509892	9.424315676877912E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1560	0.0	29.647436	1
CCTATAT	25	0.0054961555	29.6	1
TCTAGAC	45	1.3231185E-4	24.666666	3
CTAAAGT	55	1.9024801E-5	23.545454	4
ATACGGC	135	0.0	23.296297	29
ATAGGAC	40	0.0019310898	23.125	3
TATCTAT	40	0.0019310898	23.125	5
GCCTATA	50	2.7019126E-4	22.2	35
GTATCAA	2195	0.0	20.90205	2
CTCGTAT	90	9.476571E-8	20.555555	29
TCGTATG	90	9.476571E-8	20.555555	30
TATAAGA	55	5.142736E-4	20.181818	12
ATACACA	230	0.0	20.108696	37
AACGACC	120	2.382876E-10	20.041668	37
TCTAAAG	65	6.901491E-5	19.923077	2
TTGAATA	75	9.26467E-6	19.733334	4
TACGGCT	180	0.0	19.527777	30
GGTAATA	1190	0.0	19.12185	25
TTCTGCG	640	0.0	19.078125	18
GCGGTAA	1210	0.0	18.652893	23
>>END_MODULE