FastQCFastQC Report
Fri 10 Feb 2017
ERR1632572.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632572.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54788
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2040.37234430897276777No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1580.2883843177338103No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1300.2372782361100971No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1290.23545301890925022No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1110.20259910929400599No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1080.19712345769146528No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1060.19347302328977148No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1040.18982258888807768No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1030.1879973716872308No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1020.18617215448638388No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1020.18617215448638388No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG990.18069650288384317No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT920.16791998247791487RNA PCR Primer, Index 39 (95% over 23bp)
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA910.16609476527706798No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC890.16244433087537416No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC870.15879389647368036No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA840.15331824487113968No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT790.14419215886690515No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC780.14236694166605826No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA780.14236694166605826No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC770.14054172446521135No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC730.13324085566182375No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA720.13141563846097687No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT710.12959042126012996No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT700.12776520405928304No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC670.12228955245674235No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG650.11863911805504855No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA630.11498868365335474No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA630.11498868365335474No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA610.11133824925166096No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC600.10951303205081406No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC600.10951303205081406No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG590.10768781484996715No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA590.10768781484996715No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA580.10586259764912025No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG580.10586259764912025No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC570.10403738044827335No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA560.10221216324742644No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA560.10221216324742644No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC560.10221216324742644No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT560.10221216324742644No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT560.10221216324742644No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT550.10038694604657954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA502.6416274E-729.61
CGTGCCA1000.025.90000210
GCGGTAA1002.0008883E-1124.05000123
CGCGGTA1002.0008883E-1124.05000122
TACGGAG851.873559E-923.94117530
ATACGGA851.873559E-923.94117529
GCAGCCG1101.8189894E-1223.54545417
TGCCAGC1101.8189894E-1223.54545412
TCCGTGC1101.8189894E-1223.5454548
AGCCGCG1101.8189894E-1223.54545419
ATACACA551.8601251E-523.54545437
GTGCCAG1101.8189894E-1223.54545411
CAGCCGC1101.8189894E-1223.54545418
TAATACG952.6375346E-1023.36842227
AATACGG952.6375346E-1023.36842228
CCGTGCC1200.023.1250029
GGTAATA1053.6379788E-1122.90476425
CCGCGGT1053.6379788E-1122.90476421
AACTCCG1155.456968E-1222.521745
GTACTGG502.65684E-422.21