##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632572.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	54788
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.002354530189095	34.0	33.0	34.0	31.0	34.0
2	33.137256333503686	34.0	33.0	34.0	31.0	34.0
3	33.20500839599912	34.0	33.0	34.0	31.0	34.0
4	36.50631525151493	37.0	37.0	37.0	35.0	37.0
5	36.474574724392205	37.0	37.0	37.0	35.0	37.0
6	36.52279696283858	37.0	37.0	37.0	35.0	37.0
7	36.52235891071037	37.0	37.0	37.0	35.0	37.0
8	36.50793969482368	37.0	37.0	37.0	35.0	37.0
9	38.30895451558735	39.0	39.0	39.0	37.0	39.0
10	38.313517558589474	39.0	39.0	39.0	37.0	39.0
11	38.38216397751332	39.0	39.0	39.0	37.0	39.0
12	38.34195444257867	39.0	39.0	39.0	37.0	39.0
13	38.3739139957655	39.0	39.0	39.0	37.0	39.0
14	39.88234649923341	41.0	40.0	41.0	38.0	41.0
15	39.87170548295247	41.0	40.0	41.0	38.0	41.0
16	39.82426808790246	41.0	40.0	41.0	38.0	41.0
17	39.82618456596335	41.0	40.0	41.0	38.0	41.0
18	39.844820033584	41.0	40.0	41.0	38.0	41.0
19	39.840567277506025	41.0	40.0	41.0	38.0	41.0
20	39.813736584653576	41.0	40.0	41.0	38.0	41.0
21	39.77122727604585	41.0	40.0	41.0	38.0	41.0
22	39.734649923340875	41.0	40.0	41.0	38.0	41.0
23	39.683306563481054	41.0	40.0	41.0	38.0	41.0
24	39.65872088778565	41.0	40.0	41.0	37.0	41.0
25	39.607249762721764	41.0	40.0	41.0	37.0	41.0
26	39.514601737606775	41.0	40.0	41.0	37.0	41.0
27	39.402989705774985	41.0	39.0	41.0	36.0	41.0
28	39.36668613565014	41.0	39.0	41.0	36.0	41.0
29	39.32426808790246	41.0	39.0	41.0	36.0	41.0
30	39.26900051106082	41.0	39.0	41.0	36.0	41.0
31	39.19745199678762	41.0	39.0	41.0	35.0	41.0
32	39.11801854420676	41.0	39.0	41.0	35.0	41.0
33	39.05541359421771	40.0	39.0	41.0	35.0	41.0
34	39.002774330145286	40.0	39.0	41.0	35.0	41.0
35	38.92314010367234	40.0	39.0	41.0	35.0	41.0
36	38.862068336132	40.0	39.0	41.0	35.0	41.0
37	38.815470540994376	40.0	38.0	41.0	35.0	41.0
38	38.740253340147476	40.0	38.0	41.0	35.0	41.0
39	38.62062860480397	40.0	38.0	41.0	35.0	41.0
40	38.5398809958385	40.0	38.0	41.0	35.0	41.0
41	38.47566985471271	40.0	38.0	41.0	35.0	41.0
42	38.40169380156239	40.0	37.0	41.0	35.0	41.0
43	37.666514565233264	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	4.0
20	10.0
21	6.0
22	7.0
23	28.0
24	36.0
25	52.0
26	76.0
27	108.0
28	162.0
29	194.0
30	260.0
31	322.0
32	437.0
33	633.0
34	972.0
35	1526.0
36	2442.0
37	4601.0
38	11659.0
39	31251.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.82872161787253	16.010805285829015	13.141563846097688	28.018909250200775
2	22.53960721325838	19.465941447032197	30.687376797838944	27.307074541870485
3	22.254873329926262	19.044316273636564	29.092136964298753	29.608673432138428
4	16.31196612396875	16.0181061546324	33.91436080893626	33.755566912462584
5	18.425567642549463	31.658392348689496	31.541578447835295	18.374461560925752
6	33.24815653062714	33.3357669562678	15.410308826750382	18.005767686354677
7	29.49185953128422	27.117251952982407	19.35825363218223	24.032634883551143
8	27.213988464627292	29.40059867124188	20.398627436664963	22.98678542746587
9	28.588377016865007	12.281886544498795	17.01467474629481	42.115061692341385
10	20.692487406001312	22.054099437833102	28.02073446740162	29.232678688763965
11	40.39388187194276	18.64459370665109	18.049572899175	22.911951522231146
12	22.941154997444695	23.620135796159744	25.943637292837845	27.495071913557712
13	37.311090019712346	16.94349127546178	19.387457107395782	26.357961597430098
14	24.585675695407755	20.321968314229395	22.238446375118638	32.85390961524421
15	31.163758487259983	23.136453237935314	19.13375191647806	26.566036358326638
16	25.551215594655762	23.37920712564795	22.30962984595167	28.759947433744614
17	27.712272760458497	23.010513251076876	20.697963057603854	28.579250930860773
18	27.60823538001022	19.816383149594802	22.559684602467694	30.015696867927282
19	29.608673432138428	21.433525589545155	22.382638533985542	26.575162444330875
20	31.579908009053074	19.354603197780538	22.01576987661532	27.04971891655107
21	28.287216178725267	20.86040738847923	21.134189968606265	29.718186464189237
22	28.89866394100898	22.32605680075929	20.57202307074542	28.203256187486307
23	29.356793458421553	20.81295174125721	21.512009929181573	28.318244871139665
24	28.529970066437905	20.263561363802292	22.13805942907206	29.06840914068774
25	28.608454406074323	21.51383514638242	21.89713075856027	27.98057968898299
26	28.88223698620136	21.838723808133167	21.27838212747317	28.000657078192305
27	27.602759728407683	21.327662991896034	24.045411403957072	27.024165875739214
28	26.86354676206469	22.305979411549977	21.063006497773234	29.767467328612106
29	28.033510987807546	22.78053588377017	21.625173395634082	27.560779732788205
30	27.548003212382273	20.958969117324962	23.715047090603782	27.777980579688982
31	29.19252391034533	21.053880411769	21.871577717748412	27.882017960137258
32	25.96371468204716	21.179820398627434	22.21471855150763	30.641746367817767
33	26.81974154924436	20.774622180039426	24.16952617361466	28.23611009710156
34	26.399941593049576	20.896911732496168	23.844637511863912	28.858509162590344
35	26.109732058114915	22.935679345842157	23.977878367525733	26.97671022851719
36	26.516755493903776	21.176169964225743	24.66416003504417	27.642914506826312
37	27.020515441337515	20.876834343286852	23.897568810688472	28.20508140468716
38	26.321457253413154	19.781704022778708	25.84690078119296	28.049937942615173
39	25.436226911002407	18.849018033145946	27.06797108855954	28.646783967292105
40	25.100386946046584	20.19420311017011	28.228809228298168	26.476600715485144
41	23.589107103745345	18.99868584361539	28.739870044535298	28.672337008103966
42	21.221800394246916	20.199678761772653	30.74213331386435	27.836387530116085
43	20.281813535810763	20.90238738409871	29.272833467182597	29.542965612907935
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	1.5
2	1.0
3	1.0
4	1.0
5	1.0
6	3.0
7	5.0
8	3.5
9	2.0
10	7.5
11	13.0
12	13.0
13	19.0
14	25.0
15	51.5
16	78.0
17	73.5
18	69.0
19	69.0
20	83.0
21	97.0
22	77.5
23	58.0
24	61.5
25	65.0
26	65.0
27	82.5
28	100.0
29	135.5
30	171.0
31	185.5
32	200.0
33	200.0
34	278.0
35	356.0
36	398.0
37	440.0
38	567.5
39	695.0
40	695.0
41	937.5
42	1180.0
43	1482.5
44	1785.0
45	2726.0
46	3667.0
47	3667.0
48	4333.0
49	4999.0
50	5654.0
51	6309.0
52	6427.0
53	6545.0
54	6545.0
55	6226.0
56	5907.0
57	5802.0
58	5697.0
59	5237.0
60	4777.0
61	4777.0
62	4260.0
63	3743.0
64	3061.5
65	2380.0
66	2077.0
67	1774.0
68	1774.0
69	1491.0
70	1208.0
71	1039.5
72	871.0
73	647.0
74	423.0
75	423.0
76	370.0
77	317.0
78	301.0
79	285.0
80	247.5
81	210.0
82	210.0
83	187.0
84	164.0
85	124.5
86	85.0
87	66.0
88	47.0
89	47.0
90	36.0
91	25.0
92	17.0
93	9.0
94	5.5
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	54788.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.71862451631744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.53982300884955	47.38263853398554
2	7.423974255832663	8.4215521647076
3	2.8769106999195495	4.895232532671388
4	1.518905872888174	3.4460100751989486
5	0.9911504424778761	2.810834489304227
6	0.6242960579243766	2.1245528217857927
7	0.50201126307321	1.9931371833248157
8	0.36363636363636365	1.6499963495655985
9	0.2574416733708769	1.3141563846097686
>10	1.7345132743362832	18.588011973424837
>50	0.13193885760257443	4.897057749872235
>100	0.035398230088495575	2.4768197415492446
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	204	0.37234430897276777	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	158	0.2883843177338103	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	130	0.2372782361100971	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	129	0.23545301890925022	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	111	0.20259910929400599	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	108	0.19712345769146528	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	106	0.19347302328977148	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	104	0.18982258888807768	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	103	0.1879973716872308	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	102	0.18617215448638388	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	102	0.18617215448638388	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	99	0.18069650288384317	No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	92	0.16791998247791487	RNA PCR Primer, Index 39 (95% over 23bp)
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	91	0.16609476527706798	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	89	0.16244433087537416	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	87	0.15879389647368036	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	84	0.15331824487113968	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	79	0.14419215886690515	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	78	0.14236694166605826	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	78	0.14236694166605826	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	77	0.14054172446521135	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	73	0.13324085566182375	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	72	0.13141563846097687	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	71	0.12959042126012996	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	70	0.12776520405928304	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	67	0.12228955245674235	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	65	0.11863911805504855	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	63	0.11498868365335474	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	63	0.11498868365335474	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	61	0.11133824925166096	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	60	0.10951303205081406	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	60	0.10951303205081406	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	59	0.10768781484996715	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	59	0.10768781484996715	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	58	0.10586259764912025	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	58	0.10586259764912025	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	57	0.10403738044827335	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	56	0.10221216324742644	No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA	56	0.10221216324742644	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	56	0.10221216324742644	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	56	0.10221216324742644	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	56	0.10221216324742644	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	55	0.10038694604657954	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0018252172008469008	0.0
20	0.0018252172008469008	0.0	0.0	0.005475651602540702	0.0
21	0.0018252172008469008	0.0	0.0	0.007300868803387603	0.0
22	0.0018252172008469008	0.0	0.0	0.010951303205081404	0.0
23	0.0018252172008469008	0.0	0.0	0.010951303205081404	0.0
24	0.0018252172008469008	0.0	0.0	0.01642695480762211	0.0
25	0.0018252172008469008	0.0	0.0	0.02007738920931591	0.0
26	0.0018252172008469008	0.0	0.0	0.02372782361100971	0.0
27	0.0018252172008469008	0.0	0.0	0.06753303643133533	0.0
28	0.0018252172008469008	0.0	0.0	0.2664817113236475	0.0
29	0.0018252172008469008	0.0	0.0	0.4982842958312039	0.0
30	0.0018252172008469008	0.0	0.0	0.7994451339709425	0.0
31	0.0018252172008469008	0.0	0.0	1.5204059283054683	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	50	2.6416274E-7	29.6	1
CGTGCCA	100	0.0	25.900002	10
GCGGTAA	100	2.0008883E-11	24.050001	23
CGCGGTA	100	2.0008883E-11	24.050001	22
TACGGAG	85	1.873559E-9	23.941175	30
ATACGGA	85	1.873559E-9	23.941175	29
GCAGCCG	110	1.8189894E-12	23.545454	17
TGCCAGC	110	1.8189894E-12	23.545454	12
TCCGTGC	110	1.8189894E-12	23.545454	8
AGCCGCG	110	1.8189894E-12	23.545454	19
ATACACA	55	1.8601251E-5	23.545454	37
GTGCCAG	110	1.8189894E-12	23.545454	11
CAGCCGC	110	1.8189894E-12	23.545454	18
TAATACG	95	2.6375346E-10	23.368422	27
AATACGG	95	2.6375346E-10	23.368422	28
CCGTGCC	120	0.0	23.125002	9
GGTAATA	105	3.6379788E-11	22.904764	25
CCGCGGT	105	3.6379788E-11	22.904764	21
AACTCCG	115	5.456968E-12	22.52174	5
GTACTGG	50	2.65684E-4	22.2	1
>>END_MODULE