FastQCFastQC Report
Fri 10 Feb 2017
ERR1632571.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632571.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2126767
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA46550.21887682101518405No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA40340.18967757163807789No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA28360.13334794079464277No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA27670.13010357975274206No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA25460.11971222047361088No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA22380.10523014509817012No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT22270.10471292812047582No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA22160.10419571114278152No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT21590.10151558680382008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22950.027.6492391
GTATCAA32500.019.4107692
CTTATAC23450.019.01279437
GCGGTAA20800.018.76682723
TATTATA1002.8777868E-718.4999982
CGCGGTA22550.017.31042122
TAATACG21550.017.08352727
CGGTAAT22750.017.07692324
GGTAATA23250.017.02795625
TCTTATA43550.016.94948437
CTCTATG5800.016.9051741
CGTGCCA23600.016.85381310
ATACGGC3200.016.76562529
AGCCGCG23650.016.34883719
TCTATGG5950.016.1680682
CCGCGGT24500.016.15918421
ATACACA5950.015.85714337
AACTCCG24350.015.8028745
TATACAC7500.015.78666837
GTAATAC24950.015.6452926
Produced by FastQC (version 0.11.5)