FastQCFastQC Report
Fri 10 Feb 2017
ERR1632570.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632570.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128959
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4530.35127443606107367No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3570.27683217146534944No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2850.2210004730185563No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2590.2008390263572143No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2490.1930846237951597No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2240.17369861739002318No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2180.1690459758527904No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2140.16594421482796856No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT2010.1558634914972976No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC2010.1558634914972976No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2010.1558634914972976No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1980.1535371707286812No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1970.15276173047247577No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1960.1519862902162703No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1960.1519862902162703No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1950.1512108499600648No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1840.14268100714180476No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1780.138028365604572No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1690.13104940329872286No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1660.12872308253010648No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT1660.12872308253010648No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1550.1201932397118464No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1540.11941779945564095No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1520.11786691894323002No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1500.11631603843081911No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1420.11011251638117542No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTT1400.1085616358687645No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1360.10545987484394265No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA1360.10545987484394265No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT1350.10468443458773719No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC1320.1023581138191208No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1300.10080723330670989No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCTGT200.001835883137.037
ATGGCCA551.828812E-830.2727289
CGCCGTT250.005478488329.625
TTCTGGA250.005478488329.62
CCGTTTA250.005478488329.627
CGTAATG405.8999467E-527.7500022
GGTATCA1950.027.5128211
GACCGTG358.8265864E-426.428577
GTAATGA509.009636E-625.9000023
GCGTAAT451.3143335E-424.6666661
AAGCTGT601.322087E-624.66666634
TCTGGAC400.001921954123.1250023
GCGGTAA1950.022.76923223
CGTGCCA1950.022.76923210
AATGATG652.6523066E-622.769235
GGACCGT652.6523066E-622.769236
AGATGTT652.6523066E-622.769235
GTATCAA2400.022.3541662
GGTAATA2000.022.225
CGCGGTA2050.021.65853522
Produced by FastQC (version 0.11.5)