##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632570.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	128959
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.00658348777518	34.0	33.0	34.0	31.0	34.0
2	33.15881791887344	34.0	33.0	34.0	31.0	34.0
3	33.22094619220062	34.0	33.0	34.0	31.0	34.0
4	36.52515915911258	37.0	37.0	37.0	35.0	37.0
5	36.493970952008006	37.0	37.0	37.0	35.0	37.0
6	36.53503051357408	37.0	37.0	37.0	35.0	37.0
7	36.53903953969866	37.0	37.0	37.0	35.0	37.0
8	36.513760187346364	37.0	37.0	37.0	35.0	37.0
9	38.301871137338225	39.0	39.0	39.0	37.0	39.0
10	38.34142634480727	39.0	39.0	39.0	37.0	39.0
11	38.39469909040858	39.0	39.0	39.0	37.0	39.0
12	38.357028202762116	39.0	39.0	39.0	37.0	39.0
13	38.3954512674571	39.0	39.0	39.0	37.0	39.0
14	39.92770570491396	41.0	40.0	41.0	38.0	41.0
15	39.93780193704976	41.0	40.0	41.0	38.0	41.0
16	39.8933459471615	41.0	40.0	41.0	38.0	41.0
17	39.877922440465575	41.0	40.0	41.0	38.0	41.0
18	39.86491055296645	41.0	40.0	41.0	38.0	41.0
19	39.85812545072465	41.0	40.0	41.0	38.0	41.0
20	39.84476461511023	41.0	40.0	41.0	38.0	41.0
21	39.8069231306074	41.0	40.0	41.0	38.0	41.0
22	39.7870796144511	41.0	40.0	41.0	38.0	41.0
23	39.744314084321374	41.0	40.0	41.0	38.0	41.0
24	39.7399561100815	41.0	40.0	41.0	38.0	41.0
25	39.69913693499484	41.0	40.0	41.0	37.0	41.0
26	39.59253716297428	41.0	40.0	41.0	37.0	41.0
27	39.48926402965284	41.0	39.0	41.0	37.0	41.0
28	39.42632154405664	41.0	39.0	41.0	36.0	41.0
29	39.40345381090114	41.0	39.0	41.0	36.0	41.0
30	39.35773385339527	41.0	39.0	41.0	36.0	41.0
31	39.30933087260292	41.0	39.0	41.0	36.0	41.0
32	39.2658674462426	41.0	39.0	41.0	36.0	41.0
33	39.1910529703239	41.0	39.0	41.0	35.0	41.0
34	39.157802092137814	41.0	39.0	41.0	35.0	41.0
35	39.07206941741173	41.0	39.0	41.0	35.0	41.0
36	39.02876107910266	40.0	39.0	41.0	35.0	41.0
37	38.96652424413961	40.0	39.0	41.0	35.0	41.0
38	38.892446436464304	40.0	39.0	41.0	35.0	41.0
39	38.78938267201203	40.0	38.0	41.0	35.0	41.0
40	38.69330562426818	40.0	38.0	41.0	35.0	41.0
41	38.64301832365325	40.0	38.0	41.0	35.0	41.0
42	38.57672593615025	40.0	38.0	41.0	35.0	41.0
43	37.82941865243992	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	1.0
18	3.0
19	6.0
20	8.0
21	16.0
22	22.0
23	47.0
24	65.0
25	112.0
26	135.0
27	233.0
28	330.0
29	418.0
30	566.0
31	702.0
32	969.0
33	1344.0
34	2095.0
35	3582.0
36	5319.0
37	9903.0
38	27202.0
39	75880.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.88184616816197	16.128381888817376	12.592374320520475	28.397397622500176
2	22.12873859133523	19.12235671802666	31.560418427562247	27.188486263075863
3	21.81933792910925	19.154925208787287	28.642436743461104	30.38330011864236
4	16.14776789522251	15.585573709473554	33.85804790669903	34.40861048860491
5	17.88708038989136	32.38160965888383	32.09004412255057	17.64126582867423
6	34.304701494273374	33.3571135011903	15.380857481835312	16.957327522701014
7	30.525981125784163	26.947324343395962	19.36429407796276	23.16240045285711
8	27.53665893811211	29.873060430059166	20.387875216153972	22.20240541567475
9	28.456331081971793	12.543521584379533	17.146534945215148	41.85361238843353
10	20.184709869028143	22.500174474057648	28.40515202506223	28.90996363185198
11	40.06622259787995	19.123132158282864	18.16391256135671	22.64673268248048
12	23.529183694042295	23.004986080847402	26.046262765685217	27.41956745942509
13	36.195224838902284	16.940267837064493	19.74425980350344	27.120247520529784
14	24.20304127668484	19.84118983552912	22.476911266371484	33.47885762141456
15	30.59732162935507	23.32601834691646	18.934700176024936	27.141959847703532
16	25.84775006009662	23.30740778076753	21.762730790406255	29.082111368729596
17	27.914298342884177	23.193418063105327	21.126869780317776	27.765413813692724
18	27.055885979264726	20.2645802154173	22.990252715979498	29.689281089338472
19	28.995262060034584	21.823215130390277	22.373777712296157	26.80774509727898
20	30.139036437937637	20.373917291542273	22.03103311905334	27.456013151466745
21	29.0255042300266	20.822121759629027	21.43549500228755	28.71687900805682
22	29.28837847688025	22.082987616219107	20.40493490179049	28.223699005110152
23	29.218588853821757	21.372684341534907	21.09197496878853	28.31675183585481
24	28.939430361587792	20.701928519917185	21.98683302444963	28.37180809404539
25	28.95028652517467	21.29514031591436	22.180693088501	27.573880070409974
26	29.061174481812046	21.917818841647346	21.79219752014206	27.228809156398547
27	28.349320326615434	21.247838460285827	23.34695523383401	27.055885979264726
28	26.872882078800238	22.604858908645383	21.436270442543755	29.085988570010624
29	28.00502485286021	22.890220922928993	21.778239595530362	27.32651462868043
30	27.35443047790383	21.0167572639366	23.529183694042295	28.09962856411728
31	28.573422560658813	21.578176009429352	21.988383904962042	27.860017524949793
32	26.254856194604486	21.472716134585408	22.54204824789274	29.730379422917363
33	27.269907489977435	21.603765537884133	23.529183694042295	27.597143278096137
34	26.78525732984902	21.63168138710753	23.096488031079645	28.486573251963804
35	27.018664846966868	22.980947432905033	23.615257562481098	26.385130157647
36	27.336595352011106	21.509161826627068	23.644724292216907	27.509518529144923
37	27.233461797935774	21.101280251862995	23.591994354794934	28.07326359540629
38	26.403740723795938	20.659279305825883	25.404973673803298	27.532006296574878
39	25.82603773292287	19.564357664063774	26.289751006133734	28.31985359687963
40	25.69421288936794	20.443706914600764	27.463767554028802	26.398312642002498
41	23.891314293690243	19.717894834792453	27.93368434928931	28.457106522227992
42	22.221791422079885	20.501089493559967	29.39771555300522	27.879403531354928
43	21.30211927822021	20.977985251126327	28.90065834877752	28.819237121875947
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	1.5
2	0.0
3	0.5
4	1.0
5	1.0
6	5.0
7	9.0
8	8.0
9	7.0
10	10.5
11	14.0
12	14.0
13	31.0
14	48.0
15	73.0
16	98.0
17	96.0
18	94.0
19	94.0
20	109.5
21	125.0
22	116.0
23	107.0
24	119.0
25	131.0
26	131.0
27	170.0
28	209.0
29	287.0
30	365.0
31	497.5
32	630.0
33	630.0
34	827.5
35	1025.0
36	1184.5
37	1344.0
38	1835.5
39	2327.0
40	2327.0
41	2849.5
42	3372.0
43	4092.5
44	4813.0
45	6752.5
46	8692.0
47	8692.0
48	9760.5
49	10829.0
50	12462.5
51	14096.0
52	14862.5
53	15629.0
54	15629.0
55	14637.0
56	13645.0
57	13124.0
58	12603.0
59	11580.5
60	10558.0
61	10558.0
62	9957.0
63	9356.0
64	7404.0
65	5452.0
66	4665.0
67	3878.0
68	3878.0
69	3387.0
70	2896.0
71	2524.5
72	2153.0
73	2005.0
74	1857.0
75	1857.0
76	1707.5
77	1558.0
78	1061.0
79	564.0
80	380.0
81	196.0
82	196.0
83	167.0
84	138.0
85	109.0
86	80.0
87	56.0
88	32.0
89	32.0
90	23.5
91	15.0
92	10.0
93	5.0
94	4.5
95	4.0
96	4.0
97	2.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	128959.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.99728595910328
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.79669954711831	41.396102637272314
2	7.424468019107885	7.42406501291108
3	2.877039518580557	4.315325025783388
4	1.518394441342515	3.0366240433005838
5	0.9678019728270985	2.419373599361037
6	0.6638128916185868	1.9913305779356227
7	0.5226751039146349	1.8292635643886817
8	0.3939450338110304	1.5756946006094958
9	0.3396612693095105	1.528392744980963
>10	2.211675662261927	20.937662357803642
>50	0.19852348160555866	6.685070448747277
>100	0.08530305850238849	6.861095386905916
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	453	0.35127443606107367	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	357	0.27683217146534944	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	285	0.2210004730185563	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	259	0.2008390263572143	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	249	0.1930846237951597	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	224	0.17369861739002318	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	218	0.1690459758527904	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	214	0.16594421482796856	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	201	0.1558634914972976	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	201	0.1558634914972976	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	201	0.1558634914972976	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	198	0.1535371707286812	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	197	0.15276173047247577	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	196	0.1519862902162703	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	196	0.1519862902162703	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	195	0.1512108499600648	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	184	0.14268100714180476	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	178	0.138028365604572	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	169	0.13104940329872286	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	166	0.12872308253010648	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT	166	0.12872308253010648	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	155	0.1201932397118464	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	154	0.11941779945564095	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	152	0.11786691894323002	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	150	0.11631603843081911	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	142	0.11011251638117542	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTT	140	0.1085616358687645	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	136	0.10545987484394265	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	136	0.10545987484394265	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	135	0.10468443458773719	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	132	0.1023581138191208	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	130	0.10080723330670989	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	7.754402562054607E-4
15	0.0	0.0	0.0	0.0	7.754402562054607E-4
16	0.0	0.0	0.0	7.754402562054607E-4	7.754402562054607E-4
17	0.0	0.0	0.0	7.754402562054607E-4	7.754402562054607E-4
18	0.0	0.0	0.0	7.754402562054607E-4	7.754402562054607E-4
19	0.0	0.0	0.0	0.0015508805124109213	7.754402562054607E-4
20	0.0	0.0	0.0	0.0031017610248218427	7.754402562054607E-4
21	0.0	0.0	0.0	0.006203522049643685	7.754402562054607E-4
22	0.0	0.0	0.0	0.008529842818260067	7.754402562054607E-4
23	0.0	0.0	0.0	0.010080723330670989	7.754402562054607E-4
24	0.0	0.0	0.0	0.01163160384308191	7.754402562054607E-4
25	0.0	0.0	0.0	0.013957924611698291	7.754402562054607E-4
26	0.0	0.0	0.0	0.018610566148931057	7.754402562054607E-4
27	0.0	0.0	0.0	0.04187377383509488	7.754402562054607E-4
28	7.754402562054607E-4	0.0	0.0	0.16051613303453036	7.754402562054607E-4
29	7.754402562054607E-4	0.0	0.0	0.3055234609449515	7.754402562054607E-4
30	7.754402562054607E-4	0.0	0.0	0.5210958521700696	7.754402562054607E-4
31	7.754402562054607E-4	0.0	0.0	1.0282337797284409	7.754402562054607E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCTGT	20	0.0018358831	37.0	37
ATGGCCA	55	1.828812E-8	30.272728	9
CGCCGTT	25	0.0054784883	29.6	25
TTCTGGA	25	0.0054784883	29.6	2
CCGTTTA	25	0.0054784883	29.6	27
CGTAATG	40	5.8999467E-5	27.750002	2
GGTATCA	195	0.0	27.512821	1
GACCGTG	35	8.8265864E-4	26.42857	7
GTAATGA	50	9.009636E-6	25.900002	3
GCGTAAT	45	1.3143335E-4	24.666666	1
AAGCTGT	60	1.322087E-6	24.666666	34
TCTGGAC	40	0.0019219541	23.125002	3
GCGGTAA	195	0.0	22.769232	23
CGTGCCA	195	0.0	22.769232	10
AATGATG	65	2.6523066E-6	22.76923	5
GGACCGT	65	2.6523066E-6	22.76923	6
AGATGTT	65	2.6523066E-6	22.76923	5
GTATCAA	240	0.0	22.354166	2
GGTAATA	200	0.0	22.2	25
CGCGGTA	205	0.0	21.658535	22
>>END_MODULE