FastQCFastQC Report
Fri 10 Feb 2017
ERR1632569.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632569.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173933
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4430.25469577365997254No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3700.21272558973857747No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2730.15695698918549097No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2620.1506327148959657No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT2470.1420087045011585No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2450.14085883644851752No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC2310.13280976008003081No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT2300.13223482605371034No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2180.12533561773786459No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2120.12188601357994171No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2050.11786147539569833No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1980.11383693721145499No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1900.10923746500089114No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1870.10751266292192971No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1860.10693772889560924No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1820.10463799279032732No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1790.10291319071136587No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1760.10118838863240444No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1750.10061345460608395No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1740.10003852057976347No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1740.10003852057976347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2350.033.8510631
CAAGCCA250.00548368329.633
GTATCAA3200.024.8593772
GTTAATA400.001924638623.1250021
GTCAAAA400.001924638623.1250022
CTTATAC2150.022.37209337
TAATACG1900.021.42105327
TAACGTC705.060958E-621.14285727
TTCTGCG705.060958E-621.14285718
GTAACGT705.060958E-621.14285726
GGTAATA2050.020.75609625
GCGGTAA2050.020.75609623
CGTGCCA2150.020.65116310
ATAGAGC450.003812959220.55555732
CGCGGTA2100.020.26190422
GTAATAC2100.020.26190426
GCCCATA555.1190093E-420.18181828
TGGTGGT656.859176E-519.9230774
GCTAACT2050.019.8536572
CGGTAAT2150.019.79069724
Produced by FastQC (version 0.11.5)