##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632569.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	173933
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.053601099273855	34.0	33.0	34.0	31.0	34.0
2	33.193310067669735	34.0	33.0	34.0	31.0	34.0
3	33.26670039612954	34.0	34.0	34.0	31.0	34.0
4	36.544336037439706	37.0	37.0	37.0	35.0	37.0
5	36.51196725175786	37.0	37.0	37.0	35.0	37.0
6	36.55869788941719	37.0	37.0	37.0	35.0	37.0
7	36.55862314799377	37.0	37.0	37.0	35.0	37.0
8	36.535321071907	37.0	37.0	37.0	35.0	37.0
9	38.333530727349036	39.0	39.0	39.0	37.0	39.0
10	38.35845411739003	39.0	39.0	39.0	37.0	39.0
11	38.43584598667303	39.0	39.0	39.0	37.0	39.0
12	38.3882874440158	39.0	39.0	39.0	37.0	39.0
13	38.42267424813003	39.0	39.0	39.0	37.0	39.0
14	39.9757032880477	41.0	40.0	41.0	38.0	41.0
15	39.965049760539976	41.0	40.0	41.0	38.0	41.0
16	39.935480903566315	41.0	40.0	41.0	38.0	41.0
17	39.93281895902445	41.0	40.0	41.0	38.0	41.0
18	39.93211753951234	41.0	40.0	41.0	38.0	41.0
19	39.94461660524455	41.0	40.0	41.0	38.0	41.0
20	39.927799784974674	41.0	40.0	41.0	38.0	41.0
21	39.89081427906148	41.0	40.0	41.0	38.0	41.0
22	39.85820402108858	41.0	40.0	41.0	38.0	41.0
23	39.82361599006514	41.0	40.0	41.0	38.0	41.0
24	39.809673839926866	41.0	40.0	41.0	38.0	41.0
25	39.76523718903256	41.0	40.0	41.0	38.0	41.0
26	39.689886335542994	41.0	40.0	41.0	38.0	41.0
27	39.601530474378066	41.0	40.0	41.0	37.0	41.0
28	39.560560675662465	41.0	40.0	41.0	37.0	41.0
29	39.517480869070276	41.0	40.0	41.0	37.0	41.0
30	39.47694227087442	41.0	40.0	41.0	37.0	41.0
31	39.413803016103905	41.0	39.0	41.0	37.0	41.0
32	39.36000068992083	41.0	39.0	41.0	36.0	41.0
33	39.282597321957304	41.0	39.0	41.0	36.0	41.0
34	39.2481127790586	41.0	39.0	41.0	36.0	41.0
35	39.182857767071226	41.0	39.0	41.0	35.0	41.0
36	39.13750697107507	41.0	39.0	41.0	35.0	41.0
37	39.082192568402775	41.0	39.0	41.0	35.0	41.0
38	39.0107972610143	41.0	39.0	41.0	35.0	41.0
39	38.93829233095502	40.0	39.0	41.0	35.0	41.0
40	38.858445493379634	40.0	38.0	41.0	35.0	41.0
41	38.81255425939873	40.0	38.0	41.0	35.0	41.0
42	38.754761891073	40.0	38.0	41.0	35.0	41.0
43	38.02509012090862	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	1.0
19	6.0
20	7.0
21	12.0
22	32.0
23	57.0
24	85.0
25	151.0
26	193.0
27	263.0
28	382.0
29	529.0
30	763.0
31	894.0
32	1209.0
33	1763.0
34	2653.0
35	3984.0
36	6727.0
37	12602.0
38	34231.0
39	107388.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.19594326551028	16.30742872255409	12.554834332760315	27.941793679175316
2	21.683636802676894	19.197047138840816	31.56617778109962	27.553138277382672
3	21.36857295625327	19.59202681492299	28.46498364312695	30.574416585696788
4	16.129774108421056	15.42375512409951	33.30190360656115	35.144567160918285
5	17.323337147062375	32.83218250705732	33.06733052382239	16.77714982205792
6	35.682130475527934	32.99488883650601	14.757981521620394	16.56499916634566
7	30.49105115188032	27.307066514117505	19.326982228789248	22.874900105212927
8	27.56118735375116	30.734823179040205	19.92203894603094	21.781950521177695
9	27.737117165805223	12.743987627419754	17.208925275824598	42.30996993095042
10	19.507511513053878	23.17674046903118	29.48319180374052	27.832556214174424
11	39.99471060695785	18.93660202491764	18.350744252097073	22.717943116027435
12	23.093949969241027	23.710279245456583	26.089356246370727	27.106414538931656
13	35.53782203492149	17.373931341378576	20.08704501158492	27.00120161211501
14	24.551982659989765	19.454617582632395	22.228674259628708	33.76472549774913
15	29.679244306715802	23.99602145653786	19.418971673000524	26.90576256374581
16	26.52343143624269	23.52745022508667	21.665238913834635	28.283879424836
17	27.3967562222235	23.092800101188388	21.36684815417431	28.143595522413804
18	26.545278929242873	21.02188773838202	23.308400361058567	29.124432971316544
19	28.843290232445828	22.05906872186417	22.467271880551706	26.6303691651383
20	30.242679652509874	20.618858985931364	22.006749725469003	27.13171163608976
21	27.762989196989647	22.11253758631197	21.181144463672794	28.94332875302559
22	28.787521631892744	22.499468186025652	20.751668746011394	27.961341436070207
23	28.49143060833769	21.874514899415292	21.555426514807426	28.07862797743959
24	28.545474406811817	21.180569529646473	21.887738382020665	28.386217681521046
25	28.951377829394076	21.72790672270357	22.11483732241725	27.2058781254851
26	28.574796042154162	22.1470336278912	21.627293268097485	27.650877061857155
27	27.57671057246181	21.603146038992023	23.210661576584087	27.609481811962077
28	26.82527180006094	22.576509345552598	21.756653424019593	28.841565430366867
29	27.524391576066648	23.284253131953108	21.732506194914134	27.458849097066114
30	27.223701080301037	22.15910724244393	23.040481104793226	27.57671057246181
31	28.15221953280861	22.092414895390753	22.18670407570731	27.568661496093323
32	25.958271288369662	22.13553494736479	22.513841536683664	29.392352227581885
33	26.36302484289928	21.70375949359811	23.756273967562223	28.17694169594039
34	26.671764415033376	21.379496702753357	23.54699798198157	28.401740900231697
35	26.179045954476727	22.729441796553846	23.88908372764225	27.202428521327178
36	26.47513697803177	21.36684815417431	24.754359437254575	27.40365543053935
37	27.011550424588776	20.716022836379526	24.377202715988343	27.895224023043358
38	25.63745810168283	20.333691708876405	26.21354199605595	27.81530819338481
39	24.933738853466565	19.397699114026665	27.165632743642664	28.502929288864102
40	24.498513795541964	20.02265240063703	28.59319393099642	26.885639872824598
41	22.9893119764507	19.60812496765996	29.394651963687167	28.00791109220217
42	21.58417321612345	20.142813612138006	30.28464983643127	27.988363335307277
43	20.65163022543163	20.70049961766887	30.07019944461373	28.577670712285762
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	2.0
2	3.0
3	6.0
4	9.0
5	9.0
6	6.5
7	4.0
8	7.5
9	11.0
10	11.5
11	12.0
12	12.0
13	22.0
14	32.0
15	57.0
16	82.0
17	109.5
18	137.0
19	137.0
20	138.0
21	139.0
22	146.0
23	153.0
24	204.5
25	256.0
26	256.0
27	312.5
28	369.0
29	588.0
30	807.0
31	972.0
32	1137.0
33	1137.0
34	1440.5
35	1744.0
36	2029.0
37	2314.0
38	3033.0
39	3752.0
40	3752.0
41	4497.5
42	5243.0
43	6080.5
44	6918.0
45	9144.5
46	11371.0
47	11371.0
48	13074.5
49	14778.0
50	16678.5
51	18579.0
52	19531.0
53	20483.0
54	20483.0
55	19160.0
56	17837.0
57	17089.5
58	16342.0
59	15416.5
60	14491.0
61	14491.0
62	13268.5
63	12046.0
64	10100.5
65	8155.0
66	7003.0
67	5851.0
68	5851.0
69	4978.5
70	4106.0
71	3435.5
72	2765.0
73	2229.5
74	1694.0
75	1694.0
76	1345.0
77	996.0
78	795.0
79	594.0
80	460.5
81	327.0
82	327.0
83	257.0
84	187.0
85	147.5
86	108.0
87	81.0
88	54.0
89	54.0
90	43.5
91	33.0
92	19.5
93	6.0
94	5.5
95	5.0
96	5.0
97	2.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	173933.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.87503586795938
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.20477624975895	52.2138910581169
2	6.005615462232196	7.191728824206485
3	2.0351163747450864	3.655579978000005
4	1.0586865739846214	2.5355558636102495
5	0.7027739531723587	2.103930782663129
6	0.5118344193937611	1.8387662531794569
7	0.35788417485385426	1.4999829464164702
8	0.25191846699810516	1.206690179785031
9	0.2039339969498987	1.0989499843864352
>10	1.4985150069178528	16.644064386797623
>50	0.1179618217586413	4.9051716348316345
>100	0.050983499234667	5.105688108006571
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	443	0.25469577365997254	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	370	0.21272558973857747	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	273	0.15695698918549097	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	262	0.1506327148959657	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	247	0.1420087045011585	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	245	0.14085883644851752	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	231	0.13280976008003081	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	230	0.13223482605371034	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	218	0.12533561773786459	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	212	0.12188601357994171	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	205	0.11786147539569833	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	198	0.11383693721145499	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	190	0.10923746500089114	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	187	0.10751266292192971	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	186	0.10693772889560924	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	182	0.10463799279032732	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	179	0.10291319071136587	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	176	0.10118838863240444	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	175	0.10061345460608395	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	174	0.10003852057976347	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	174	0.10003852057976347	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	5.749340263204797E-4	0.0
18	0.0	0.0	0.0	0.0011498680526409594	0.0
19	0.0	0.0	0.0	0.0017248020789614393	0.0
20	0.0	0.0	0.0	0.0017248020789614393	0.0
21	5.749340263204797E-4	0.0	0.0	0.0034496041579228785	0.0
22	5.749340263204797E-4	0.0	0.0	0.005749340263204797	0.0
23	5.749340263204797E-4	0.0	0.0	0.007474142342166237	0.0
24	5.749340263204797E-4	0.0	0.0	0.009198944421127675	0.0
25	5.749340263204797E-4	0.0	0.0	0.012073614552730073	0.0
26	5.749340263204797E-4	0.0	0.0	0.015523218710652953	0.0
27	5.749340263204797E-4	0.0	0.0	0.0396704478161131	0.0
28	5.749340263204797E-4	0.0	0.0	0.14660817671172233	0.0
29	5.749340263204797E-4	0.0	0.0	0.3150638464236229	0.0
30	5.749340263204797E-4	0.0	0.0	0.5571110715045449	0.0
31	5.749340263204797E-4	0.0	0.0	1.2205849378783784	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	235	0.0	33.851063	1
CAAGCCA	25	0.005483683	29.6	33
GTATCAA	320	0.0	24.859377	2
GTTAATA	40	0.0019246386	23.125002	1
GTCAAAA	40	0.0019246386	23.125002	2
CTTATAC	215	0.0	22.372093	37
TAATACG	190	0.0	21.421053	27
TAACGTC	70	5.060958E-6	21.142857	27
TTCTGCG	70	5.060958E-6	21.142857	18
GTAACGT	70	5.060958E-6	21.142857	26
GGTAATA	205	0.0	20.756096	25
GCGGTAA	205	0.0	20.756096	23
CGTGCCA	215	0.0	20.651163	10
ATAGAGC	45	0.0038129592	20.555557	32
CGCGGTA	210	0.0	20.261904	22
GTAATAC	210	0.0	20.261904	26
GCCCATA	55	5.1190093E-4	20.181818	28
TGGTGGT	65	6.859176E-5	19.923077	4
GCTAACT	205	0.0	19.853657	2
CGGTAAT	215	0.0	19.790697	24
>>END_MODULE