FastQCFastQC Report
Fri 10 Feb 2017
ERR1632567.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632567.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences654467
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA21820.33340107293415866No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA17330.26479562758702885No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA12770.19512060959528899No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA12170.18595284407006007No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT11740.17938261211031267No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11680.17846583555778978No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA11030.16853408957212512No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT10260.15676879048141465No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA10260.15676879048141465No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA10190.15569921783680463No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT10110.15447684910010742No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC10000.15279609208714878No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA9720.1485178015087086No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG9590.1465314523115757No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC9170.14011401644391544No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT8740.13354378448416804No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT8570.1309462509186865No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC8380.12804312516903066No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA8150.12452881505102624No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT8100.12376483459059051No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC7960.12162568930137042No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC7940.12132009711719612No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC7750.1184169713675403No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA7640.11673621435458166No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG7640.11673621435458166No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA7430.11352749642075154No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA7410.11322190423657724No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC7220.11031877848692141No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC7190.10986039021065998No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC6770.10344295434299973No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC6640.10145660514586678No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT6620.1011510129616925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC1550.031.03225929
ATAAGAG250.00549463529.613
GGTATCA8800.028.1704541
GTGTATA358.865298E-426.428579
GCGGTAA9250.024.423
TTAATAA400.00193030323.12500237
GATATGT400.00193030323.1250021
GCCGTCT802.7212081E-823.12500236
CGTGCCA10200.022.85294210
CGGTAAT10050.022.27363224
CTCGTAT753.7356767E-722.20000329
TTTAGGA502.7003794E-422.22
GTTTAGG502.7003794E-422.21
TACGGCT2250.022.230
GTGCCAG10500.022.211
AACTCCG10350.022.1642515
TAATACG9550.021.8900527
CGCGGTA10350.021.80676522
GGTAATA10300.021.73301125
AGCCGCG10700.021.6121519
Produced by FastQC (version 0.11.5)