FastQCFastQC Report
Fri 10 Feb 2017
ERR1632566.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632566.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47873
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3020.6308357529296262No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2260.47208238464270047No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1680.3509284983184676No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1630.3404841977732751No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1470.3070624360286591No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1430.29870699559250513No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1420.2966181354834667No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1370.2861738349382742No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1350.28199611472019714No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1270.2652852338478892No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1260.26319637373885074No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT1260.26319637373885074Illumina PCR Primer Index 10 (95% over 22bp)
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1230.2569297934117352No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1210.2527520731936582No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1150.24021891253942726No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1080.22559689177615774No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1080.22559689177615774No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1030.21515259123096528No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1020.21306373112192678No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1020.21306373112192678No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1010.21097487101288825No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA990.20679715079481129No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA980.2047082906857728No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC940.1963528502496188No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA900.1879974098134648No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA880.1838196895953878No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA870.18173082948634928No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC860.1796419693773108No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC850.17755310926827228No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT830.1733753890501953No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC820.1712865289411568No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT780.16293108850500282No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG770.16084222839596432No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC760.15875336828692582No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC740.15457564806884883No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG710.14830906774173336No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG710.14830906774173336No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC700.14622020763269483No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG690.14413134752365636No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA680.14204248741461784No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA680.14204248741461784No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC670.13995362730557936No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA650.13577590708750237No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC640.13368704697846384No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT610.12742046665134837No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA610.12742046665134837No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC610.12742046665134837No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC600.12533160654230985No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA590.12324274643327136No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT590.12324274643327136No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG590.12324274643327136No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG580.12115388632423287No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA580.12115388632423287No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT570.11906502621519437No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA570.11906502621519437No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA570.11906502621519437No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG570.11906502621519437No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG570.11906502621519437No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA560.11697616610615587No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT560.11697616610615587No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA560.11697616610615587No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC550.11488730599711738No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT540.11279844588807887No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG540.11279844588807887No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC540.11279844588807887No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC540.11279844588807887No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC530.11070958577904039No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA510.10653186556096339No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC500.10444300545192488No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC500.10444300545192488No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA500.10444300545192488No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT490.1023541453428864No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT490.1023541453428864No Hit
CAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAAT490.1023541453428864No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA490.1023541453428864No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTAC250.00544451229.5999988
AGGAATA250.00544451229.5999988
ATACCGG250.00544451229.59999812
GGAATAC250.00544451229.5999989
GGTATCA900.028.7777791
TTATACA358.7452686E-426.42857237
TGGTTCA551.8537501E-523.5454545
GCCTTGG551.8537501E-523.54545436
GTACTGG551.8537501E-523.5454541
CTGGTTC551.8537501E-523.5454544
TACTGGT551.8537501E-523.5454542
ACTGGTT551.8537501E-523.5454543
CGGTGGC400.001904415823.12516
GCTTCTT753.5883204E-722.213
CCTTGGA502.650038E-422.19999937
CCAGCAG1350.021.92592414
GCGGTAA1350.021.92592423
GGTAATA1350.021.92592425
TCGGTCA603.6311696E-521.58333415
TCAATGA704.935506E-621.14285932
Produced by FastQC (version 0.11.5)