##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632566.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	47873
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.97221816054979	34.0	33.0	34.0	31.0	34.0
2	33.10945626971362	34.0	33.0	34.0	31.0	34.0
3	33.178325987508615	34.0	33.0	34.0	31.0	34.0
4	36.483717335450045	37.0	37.0	37.0	35.0	37.0
5	36.46696467737556	37.0	37.0	37.0	35.0	37.0
6	36.51072629665991	37.0	37.0	37.0	35.0	37.0
7	36.51730620600338	37.0	37.0	37.0	35.0	37.0
8	36.49117456603931	37.0	37.0	37.0	35.0	37.0
9	38.28646627535354	39.0	39.0	39.0	37.0	39.0
10	38.32237378062791	39.0	39.0	39.0	37.0	39.0
11	38.39222526267416	39.0	39.0	39.0	37.0	39.0
12	38.31786184279239	39.0	39.0	39.0	37.0	39.0
13	38.374114845528794	39.0	39.0	39.0	37.0	39.0
14	39.856578864913416	41.0	40.0	41.0	38.0	41.0
15	39.89474233910555	41.0	40.0	41.0	38.0	41.0
16	39.81532387775991	41.0	40.0	41.0	38.0	41.0
17	39.84354437783302	41.0	40.0	41.0	38.0	41.0
18	39.8729137509661	41.0	40.0	41.0	38.0	41.0
19	39.86146679756857	41.0	40.0	41.0	38.0	41.0
20	39.854490004804376	41.0	40.0	41.0	38.0	41.0
21	39.81363190107159	41.0	40.0	41.0	38.0	41.0
22	39.76569256156915	41.0	40.0	41.0	38.0	41.0
23	39.72356025316984	41.0	40.0	41.0	38.0	41.0
24	39.69469220646293	41.0	40.0	41.0	38.0	41.0
25	39.65868025818311	41.0	40.0	41.0	37.0	41.0
26	39.55154262319053	41.0	40.0	41.0	37.0	41.0
27	39.406638397426526	41.0	39.0	41.0	37.0	41.0
28	39.361707016481105	41.0	39.0	41.0	37.0	41.0
29	39.30984061997368	41.0	39.0	41.0	36.0	41.0
30	39.29296263029265	41.0	39.0	41.0	36.0	41.0
31	39.22219204979842	41.0	39.0	41.0	36.0	41.0
32	39.184509013431374	41.0	39.0	41.0	35.0	41.0
33	39.089674764481025	41.0	39.0	41.0	35.0	41.0
34	39.06057694316212	41.0	39.0	41.0	35.0	41.0
35	38.97541411651662	40.0	39.0	41.0	35.0	41.0
36	38.90449731581476	40.0	39.0	41.0	35.0	41.0
37	38.823888204206966	40.0	38.0	41.0	35.0	41.0
38	38.733649447496504	40.0	38.0	41.0	35.0	41.0
39	38.57040502997514	40.0	38.0	41.0	35.0	41.0
40	38.51047563344683	40.0	38.0	41.0	35.0	41.0
41	38.43412779646147	40.0	38.0	41.0	35.0	41.0
42	38.34898585841706	40.0	37.0	41.0	35.0	41.0
43	37.578572473001486	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	0.0
21	4.0
22	9.0
23	23.0
24	32.0
25	46.0
26	69.0
27	86.0
28	112.0
29	183.0
30	231.0
31	290.0
32	417.0
33	570.0
34	871.0
35	1296.0
36	2102.0
37	3859.0
38	10356.0
39	27315.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.36264700353018	15.618407035280848	12.533160654230985	28.485785306957993
2	22.83124099179078	19.80030497357592	30.36575940509264	27.002694629540656
3	22.08134021264596	18.611743571533015	28.771959141896268	30.534957073924758
4	15.54320807135546	16.0988448603597	35.698619263467926	32.65932780481691
5	18.112506005472813	32.17471225951998	32.05982495352286	17.652956781484345
6	31.199214588599	35.13462703402753	16.286842270173167	17.379316107200303
7	28.358364840306642	26.985983748668353	19.29897854740668	25.356672863618325
8	26.743675976019887	28.99128945334531	21.33143943350114	22.933595137133665
9	27.620997221816058	12.117477492532325	17.04509848975414	43.216426795897476
10	20.84055730787709	21.224907567940175	26.67265473231258	31.261880391870157
11	41.348985858417066	18.582499530006473	17.554780356359533	22.513734255216928
12	21.864098761305957	23.269901614688866	26.440791260209306	28.42520836379588
13	38.58333507405009	16.345330353226245	18.72663087753013	26.344703695193534
14	24.176466902011573	20.069767927641884	22.48657907379943	33.267186096547114
15	31.608631169970547	22.597288659578467	18.53236688738955	27.261713283061432
16	24.61303866480062	23.7169176780231	22.557600317506736	29.112443339669543
17	27.877927015227787	23.071459904330208	20.084389948405153	28.966223132036852
18	27.09669333444739	19.405510412967644	22.31738140496731	31.180414847617655
19	29.450838677333778	22.04374073068327	22.02702984981096	26.478390742171996
20	32.35017650867921	18.71618657698494	21.0473544586719	27.886282455663945
21	28.803292043531847	20.253587617237272	20.86980134940363	30.073318989827253
22	29.43830551667955	22.058362751446538	19.91101455935496	28.592317172518953
23	30.05660810895494	20.625404716646127	20.711047981116703	28.606939193282226
24	28.76569256156915	19.40759927307668	22.189960938315963	29.636747227038207
25	29.889499300231865	21.172686065214215	21.63223528920268	27.305579345351244
26	29.95216510350302	21.728322854218455	21.110020261943056	27.20949178033547
27	27.89254903599106	20.842646167986132	24.54410628120235	26.720698514820462
28	26.937939966160467	23.11323710651098	20.56482777348401	29.383995153844545
29	28.061746704823175	24.017713533724645	20.42696300628747	27.493576755164707
30	28.032502663296636	20.792513525369205	23.73362855889541	27.441355252438743
31	29.14586510141416	21.790988657489606	21.32726171328306	27.73588452781317
32	26.392747477701416	20.932467152674786	22.129383995153844	30.54540137446995
33	26.472124161844878	21.229085288158252	23.675140475842333	28.623650074154533
34	26.76665343721931	21.266684770120943	22.795730369937125	29.170931422722617
35	25.9143985127316	23.391055501013096	23.781672341403297	26.912873644852002
36	27.802728051302406	21.21864098761306	23.99682493263426	26.981806028450272
37	28.400142042487413	21.178952645541326	23.430743843084826	26.99016146888643
38	26.93376224594239	19.90057025880977	25.11436509096986	28.051302404277983
39	26.03137467883776	19.100536837048026	26.081507321454684	28.786581162659537
40	25.979153176111797	20.38309694399766	27.092515614229317	26.54523426566123
41	23.16128088901886	19.714661709105343	27.842416393374137	29.281641008501662
42	21.44006015917114	20.378919223779583	30.695799302320726	27.485221314728552
43	20.51051741064901	21.99569694817538	28.65289411568107	28.840891525494538
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.5
2	2.0
3	1.5
4	1.0
5	1.0
6	2.5
7	4.0
8	4.5
9	5.0
10	6.5
11	8.0
12	8.0
13	14.0
14	20.0
15	28.5
16	37.0
17	38.5
18	40.0
19	40.0
20	47.0
21	54.0
22	38.5
23	23.0
24	23.5
25	24.0
26	24.0
27	21.5
28	19.0
29	21.5
30	24.0
31	28.0
32	32.0
33	32.0
34	72.5
35	113.0
36	117.5
37	122.0
38	231.5
39	341.0
40	341.0
41	574.5
42	808.0
43	1068.0
44	1328.0
45	2346.0
46	3364.0
47	3364.0
48	3938.0
49	4512.0
50	5227.5
51	5943.0
52	6203.5
53	6464.0
54	6464.0
55	5930.0
56	5396.0
57	5251.5
58	5107.0
59	4765.5
60	4424.0
61	4424.0
62	4032.0
63	3640.0
64	2849.0
65	2058.0
66	1778.5
67	1499.0
68	1499.0
69	1290.0
70	1081.0
71	851.5
72	622.0
73	478.5
74	335.0
75	335.0
76	288.5
77	242.0
78	171.0
79	100.0
80	75.5
81	51.0
82	51.0
83	32.5
84	14.0
85	10.0
86	6.0
87	6.0
88	6.0
89	6.0
90	4.0
91	2.0
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	47873.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.573224991122345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.7140294223179	37.59112652225681
2	7.965554359526372	7.4196311073047445
3	3.0229637603157515	4.223675140475843
4	1.7536777897380698	3.26697721053621
5	1.2199497667743093	2.8408497482923565
6	0.897021887334051	2.506632130846197
7	0.5965195550771438	1.9447287615148412
8	0.493362038033728	1.8381968959538781
9	0.3857194115536419	1.6167777243957973
>10	2.6462145676354503	24.01144695339753
>50	0.210800143523502	6.630041986088192
>100	0.09418729817007535	6.109915818937606
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	302	0.6308357529296262	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	226	0.47208238464270047	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	168	0.3509284983184676	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	163	0.3404841977732751	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	147	0.3070624360286591	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	143	0.29870699559250513	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	142	0.2966181354834667	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	137	0.2861738349382742	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	135	0.28199611472019714	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	127	0.2652852338478892	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	126	0.26319637373885074	No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT	126	0.26319637373885074	Illumina PCR Primer Index 10 (95% over 22bp)
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	123	0.2569297934117352	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	121	0.2527520731936582	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	115	0.24021891253942726	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	108	0.22559689177615774	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	108	0.22559689177615774	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	103	0.21515259123096528	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	102	0.21306373112192678	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	102	0.21306373112192678	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	101	0.21097487101288825	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	99	0.20679715079481129	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	98	0.2047082906857728	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	94	0.1963528502496188	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	90	0.1879974098134648	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	88	0.1838196895953878	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	87	0.18173082948634928	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	86	0.1796419693773108	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	85	0.17755310926827228	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	83	0.1733753890501953	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	82	0.1712865289411568	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	78	0.16293108850500282	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	77	0.16084222839596432	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	76	0.15875336828692582	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	74	0.15457564806884883	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	71	0.14830906774173336	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	71	0.14830906774173336	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	70	0.14622020763269483	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	69	0.14413134752365636	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	68	0.14204248741461784	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	68	0.14204248741461784	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	67	0.13995362730557936	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	65	0.13577590708750237	No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC	64	0.13368704697846384	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	61	0.12742046665134837	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	61	0.12742046665134837	No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC	61	0.12742046665134837	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	60	0.12533160654230985	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	59	0.12324274643327136	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	59	0.12324274643327136	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	59	0.12324274643327136	No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG	58	0.12115388632423287	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	58	0.12115388632423287	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT	57	0.11906502621519437	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	57	0.11906502621519437	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	57	0.11906502621519437	No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG	57	0.11906502621519437	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	57	0.11906502621519437	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	56	0.11697616610615587	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	56	0.11697616610615587	No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA	56	0.11697616610615587	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	55	0.11488730599711738	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	54	0.11279844588807887	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	54	0.11279844588807887	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	54	0.11279844588807887	No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC	54	0.11279844588807887	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	53	0.11070958577904039	No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA	51	0.10653186556096339	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC	50	0.10444300545192488	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	50	0.10444300545192488	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	50	0.10444300545192488	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	49	0.1023541453428864	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	49	0.1023541453428864	No Hit
CAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAAT	49	0.1023541453428864	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	49	0.1023541453428864	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0020888601090384975	0.0
22	0.0	0.0	0.0	0.004177720218076995	0.0
23	0.0	0.0	0.0	0.004177720218076995	0.0
24	0.0	0.0	0.0	0.004177720218076995	0.0
25	0.0	0.0	0.0	0.004177720218076995	0.0
26	0.0	0.0	0.0	0.010444300545192488	0.0
27	0.0	0.0	0.0	0.02715518141750047	0.0
28	0.0	0.0	0.0	0.12533160654230985	0.0
29	0.0	0.0	0.0	0.29244041526538966	0.0
30	0.0	0.0	0.0	0.5431036283500094	0.0
31	0.0	0.0	0.0	1.0736740960457878	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCTAC	25	0.005444512	29.599998	8
AGGAATA	25	0.005444512	29.599998	8
ATACCGG	25	0.005444512	29.599998	12
GGAATAC	25	0.005444512	29.599998	9
GGTATCA	90	0.0	28.777779	1
TTATACA	35	8.7452686E-4	26.428572	37
TGGTTCA	55	1.8537501E-5	23.545454	5
GCCTTGG	55	1.8537501E-5	23.545454	36
GTACTGG	55	1.8537501E-5	23.545454	1
CTGGTTC	55	1.8537501E-5	23.545454	4
TACTGGT	55	1.8537501E-5	23.545454	2
ACTGGTT	55	1.8537501E-5	23.545454	3
CGGTGGC	40	0.0019044158	23.125	16
GCTTCTT	75	3.5883204E-7	22.2	13
CCTTGGA	50	2.650038E-4	22.199999	37
CCAGCAG	135	0.0	21.925924	14
GCGGTAA	135	0.0	21.925924	23
GGTAATA	135	0.0	21.925924	25
TCGGTCA	60	3.6311696E-5	21.583334	15
TCAATGA	70	4.935506E-6	21.142859	32
>>END_MODULE