FastQCFastQC Report
Fri 10 Feb 2017
ERR1632565.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632565.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136526
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3380.2475718910683679No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2470.180917920396115No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG2220.16260638999164995No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1990.14575978201954207No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG1960.14356239837100626No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC1920.14063255350629184No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC1680.12305348431800536No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1580.11572887215621933No Hit
GAGCTGGAGAGTGGCTGGTCAAACTCTAGGCGCGGATCAGCAG1530.11206656607532632No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1510.11060164364296911No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1500.1098691824267905No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG1490.1091367212106119No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT1490.1091367212106119RNA PCR Primer, Index 26 (100% over 22bp)
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1450.10620687634589748No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT1420.10400949269736166No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA1410.10327703148118308No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1410.10327703148118308No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA1400.10254457026500448No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACAATA303.5830296E-430.83333434
CTCCAGA250.005479600329.61
GACTAAG250.005479600329.61
GGTATCA1300.028.4615361
ACAATAT358.829247E-426.4285735
GGAATTA400.001922528523.12516
CGGAATT400.001922528523.12515
TCACAAT400.001922528523.12533
AACTCCG1450.021.6896555
CGGTAAT1450.021.68965524
TAACTCC1450.021.6896554
GTATCAA1750.021.1428592
GGATACC705.04779E-621.1428571
TAATACG1500.020.96666527
GCGGTAA1500.020.96666523
AATACGG1500.020.96666528
TCTGCGG450.003808818520.55555519
AGACTGA450.003808818520.5555556
CTCACAA450.003808818520.55555532
GGTAACG450.003808818520.55555525
Produced by FastQC (version 0.11.5)