##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632565.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	136526
Sequences flagged as poor quality	0
Sequence length	43
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.03548774592385	34.0	33.0	34.0	31.0	34.0
2	33.16960871921832	34.0	33.0	34.0	31.0	34.0
3	33.254515623397744	34.0	34.0	34.0	31.0	34.0
4	36.53717240672106	37.0	37.0	37.0	35.0	37.0
5	36.50396993979169	37.0	37.0	37.0	35.0	37.0
6	36.53893763825206	37.0	37.0	37.0	35.0	37.0
7	36.53386168202394	37.0	37.0	37.0	35.0	37.0
8	36.507961853419864	37.0	37.0	37.0	35.0	37.0
9	38.31498029679329	39.0	39.0	39.0	37.0	39.0
10	38.33555513235574	39.0	39.0	39.0	37.0	39.0
11	38.40408420374141	39.0	39.0	39.0	37.0	39.0
12	38.353932584269664	39.0	39.0	39.0	37.0	39.0
13	38.39141262470152	39.0	39.0	39.0	37.0	39.0
14	39.93927164056663	41.0	40.0	41.0	38.0	41.0
15	39.941989071678655	41.0	40.0	41.0	38.0	41.0
16	39.92097475938649	41.0	40.0	41.0	38.0	41.0
17	39.88452016465728	41.0	40.0	41.0	38.0	41.0
18	39.89057761891508	41.0	40.0	41.0	38.0	41.0
19	39.87515198570236	41.0	40.0	41.0	38.0	41.0
20	39.85744107349516	41.0	40.0	41.0	38.0	41.0
21	39.8278203419129	41.0	40.0	41.0	38.0	41.0
22	39.79108741192154	41.0	40.0	41.0	38.0	41.0
23	39.765773552290405	41.0	40.0	41.0	38.0	41.0
24	39.75146858473844	41.0	40.0	41.0	38.0	41.0
25	39.69894379092627	41.0	40.0	41.0	38.0	41.0
26	39.61213248758478	41.0	40.0	41.0	37.0	41.0
27	39.514575978201954	41.0	40.0	41.0	37.0	41.0
28	39.45337884359023	41.0	39.0	41.0	37.0	41.0
29	39.39300939015279	41.0	39.0	41.0	37.0	41.0
30	39.371995077860625	41.0	39.0	41.0	36.0	41.0
31	39.263883802352666	41.0	39.0	41.0	36.0	41.0
32	39.21157142229319	41.0	39.0	41.0	36.0	41.0
33	39.1247601189517	41.0	39.0	41.0	35.0	41.0
34	39.066419583083075	41.0	39.0	41.0	35.0	41.0
35	38.98458169139944	40.0	39.0	41.0	35.0	41.0
36	38.92755958571993	40.0	39.0	41.0	35.0	41.0
37	38.844666949885	40.0	38.0	41.0	35.0	41.0
38	38.76857887874837	40.0	38.0	41.0	35.0	41.0
39	38.64255160189268	40.0	38.0	41.0	35.0	41.0
40	38.54595461670304	40.0	38.0	41.0	35.0	41.0
41	38.47067957751637	40.0	38.0	41.0	35.0	41.0
42	38.378828941007576	40.0	37.0	41.0	35.0	41.0
43	37.58738262309011	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	0.0
18	0.0
19	4.0
20	7.0
21	12.0
22	32.0
23	51.0
24	79.0
25	104.0
26	167.0
27	245.0
28	330.0
29	478.0
30	635.0
31	821.0
32	1183.0
33	1571.0
34	2275.0
35	3568.0
36	5489.0
37	11640.0
38	28653.0
39	79180.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.447753541449984	15.712025548247222	11.20738906874881	27.63283184155399
2	21.798045793475236	18.776643276738497	31.006548203272636	28.41876272651363
3	22.139372720214464	18.630883494718955	27.670187363579096	31.559556421487482
4	15.860715175131476	14.627982948302886	32.41507112198409	37.09623075458154
5	18.417737280810982	31.85986552012071	32.29055271523373	17.43184448383458
6	37.92317946764719	31.715570660533526	14.396525203990448	15.96472466782884
7	32.72416975521146	25.928394591506382	18.46534725986259	22.88208839341957
8	29.082372588371445	29.324817250926564	19.542065247645137	22.050744913056857
9	28.669264462446716	11.602185664269077	16.403468936319822	43.32508093696439
10	19.667316115611676	22.381084921553406	29.09702181269502	28.8545771501399
11	41.70414426556114	18.132077406501327	17.39595388424183	22.767824443695705
12	23.69438788216164	22.15328948332186	25.433250809369646	28.71907182514686
13	36.52124869988134	16.330222814701962	19.58821030426439	27.56031818115231
14	25.797284033810406	17.89768981732417	21.60614095483644	34.698885194028975
15	29.623661427127434	23.06666861989658	19.27251952009141	28.037150432884577
16	28.761554575685217	21.76874734482809	21.05679504270249	28.412903036784204
17	28.44879363637695	22.002402472789065	20.719130422044152	28.829673468789828
18	27.240965090898438	20.07676193545552	22.665279873430702	30.016993100215345
19	30.194981175746744	21.442069642412434	21.598816342674656	26.764132839166166
20	31.059285410837496	20.005713197486195	21.089755797430527	27.84524559424578
21	28.385069510569416	21.22965588972064	20.584357558267293	29.800917041442652
22	29.88807992616791	21.241375269179496	19.941989071678652	28.928555732973937
23	28.24150711219841	21.1864406779661	21.267743872961926	29.304308336873564
24	29.219342835796848	20.75209117677219	20.56458110543047	29.4639848820005
25	29.44640581281221	20.95571539486984	21.368091059578393	28.229787732739553
26	29.707894466987973	21.357836602551895	20.095073465859983	28.83919546460015
27	29.196636538095305	20.92934679108741	21.524105298624438	28.349911372192842
28	27.829131447489857	21.996542783059635	20.721327805692688	29.45299796375782
29	28.971770944728476	22.70776262396906	20.86855251014459	27.45191392115787
30	28.869226374463473	20.3682814994946	21.817089785095877	28.945402340946046
31	27.9954001435624	20.992338455678773	21.2618841832325	29.75037721752633
32	26.964094751182927	21.338060149715073	21.73139182280298	29.96645327629902
33	27.644551221012847	20.463501457597822	22.6447709593777	29.24717636201163
34	28.133835313420153	20.927149407438876	22.59276621302902	28.34624906611195
35	27.99393522113004	21.515315764030294	22.229465449804433	28.261283565035235
36	27.308351522786868	20.615853390562968	22.840338104097388	29.235456982552776
37	26.720917627411627	20.277456308688453	24.316979915913453	28.684646147986463
38	26.743623925113162	20.0225598054583	24.04084203741412	29.192974232014414
39	25.65445409665558	19.91195816181533	26.171571715277675	28.26201602625141
40	26.666715497414412	19.680500417502895	26.324656109459006	27.32812797562369
41	24.23494425970145	19.58235061453496	27.067371782664107	29.11533334309948
42	22.438216896415337	20.912500183115306	28.38873181665031	28.260551103819054
43	22.61913481681145	20.063577633564304	27.536879422234595	29.780408127389656
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	2.5
4	5.0
5	5.0
6	4.0
7	3.0
8	3.0
9	3.0
10	4.0
11	5.0
12	5.0
13	12.5
14	20.0
15	33.0
16	46.0
17	48.5
18	51.0
19	51.0
20	62.5
21	74.0
22	67.5
23	61.0
24	82.5
25	104.0
26	104.0
27	129.5
28	155.0
29	222.0
30	289.0
31	379.5
32	470.0
33	470.0
34	833.0
35	1196.0
36	1424.5
37	1653.0
38	2075.5
39	2498.0
40	2498.0
41	2943.5
42	3389.0
43	4247.0
44	5105.0
45	6276.5
46	7448.0
47	7448.0
48	8332.0
49	9216.0
50	10626.0
51	12036.0
52	13252.0
53	14468.0
54	14468.0
55	14086.0
56	13704.0
57	13103.0
58	12502.0
59	12725.5
60	12949.0
61	12949.0
62	12125.0
63	11301.0
64	9281.5
65	7262.0
66	6701.5
67	6141.0
68	6141.0
69	5431.0
70	4721.0
71	4664.0
72	4607.0
73	3703.0
74	2799.0
75	2799.0
76	1871.0
77	943.0
78	778.5
79	614.0
80	483.5
81	353.0
82	353.0
83	256.0
84	159.0
85	128.0
86	97.0
87	70.5
88	44.0
89	44.0
90	32.5
91	21.0
92	16.5
93	12.0
94	7.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	136526.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.83277910434643
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.92324441297872	47.114102808256305
2	6.224870092571566	6.826538534784583
3	2.216107185316787	3.645459472920909
4	1.2302801191541657	2.698387120401975
5	0.808164464808111	2.2156951789402752
6	0.5677188389147887	1.8677761012554386
7	0.40074270982220384	1.5381685539750671
8	0.3232657859232444	1.4180449145217762
9	0.25113209815524773	1.239324377774197
>10	1.8100212393636204	19.717123478311823
>50	0.17231936522354763	6.275727700218274
>100	0.07213368776799668	5.44365175863938
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	338	0.2475718910683679	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	247	0.180917920396115	No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG	222	0.16260638999164995	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	199	0.14575978201954207	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	196	0.14356239837100626	No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC	192	0.14063255350629184	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	168	0.12305348431800536	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	158	0.11572887215621933	No Hit
GAGCTGGAGAGTGGCTGGTCAAACTCTAGGCGCGGATCAGCAG	153	0.11206656607532632	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	151	0.11060164364296911	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	150	0.1098691824267905	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	149	0.1091367212106119	No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT	149	0.1091367212106119	RNA PCR Primer, Index 26 (100% over 22bp)
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	145	0.10620687634589748	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	142	0.10400949269736166	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	141	0.10327703148118308	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	141	0.10327703148118308	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	140	0.10254457026500448	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	7.324612161786033E-4	0.0
18	0.0	0.0	0.0	7.324612161786033E-4	0.0
19	0.0	0.0	0.0	7.324612161786033E-4	0.0
20	0.0	0.0	0.0	0.0014649224323572067	0.0
21	0.0	0.0	0.0	0.0014649224323572067	0.0
22	0.0	0.0	0.0	0.0029298448647144134	0.0
23	0.0	0.0	0.0	0.00439476729707162	0.0
24	0.0	0.0	0.0	0.00659215094560743	0.0
25	0.0	0.0	0.0	0.007324612161786034	0.0
26	0.0	0.0	0.0	0.011719379458857654	0.0
27	0.0	0.0	0.0	0.02417122013389391	0.0
28	0.0	0.0	0.0	0.11206656607532631	0.0
29	0.0	0.0	0.0	0.2644184990404758	0.0
30	0.0	0.0	0.0	0.4343495011939118	0.0
31	0.0	0.0	0.0	0.9287608221144691	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACAATA	30	3.5830296E-4	30.833334	34
CTCCAGA	25	0.0054796003	29.6	1
GACTAAG	25	0.0054796003	29.6	1
GGTATCA	130	0.0	28.461536	1
ACAATAT	35	8.829247E-4	26.42857	35
GGAATTA	40	0.0019225285	23.125	16
CGGAATT	40	0.0019225285	23.125	15
TCACAAT	40	0.0019225285	23.125	33
AACTCCG	145	0.0	21.689655	5
CGGTAAT	145	0.0	21.689655	24
TAACTCC	145	0.0	21.689655	4
GTATCAA	175	0.0	21.142859	2
GGATACC	70	5.04779E-6	21.142857	1
TAATACG	150	0.0	20.966665	27
GCGGTAA	150	0.0	20.966665	23
AATACGG	150	0.0	20.966665	28
TCTGCGG	45	0.0038088185	20.555555	19
AGACTGA	45	0.0038088185	20.555555	6
CTCACAA	45	0.0038088185	20.555555	32
GGTAACG	45	0.0038088185	20.555555	25
>>END_MODULE