FastQCFastQC Report
Fri 10 Feb 2017
ERR1632563.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632563.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17598
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA580.3295829071485396No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA430.24434594840322763No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA410.23298102057051936No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT390.2216160927378111RNA PCR Primer, Index 17 (95% over 21bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT290.1647914535742698No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT280.15910898965791567No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC250.14206159790885328No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA240.13637913399249915No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT230.130696670076145No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA230.130696670076145No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA220.1250142061597909No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC210.11933174224343676No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC200.11364927832708263No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA200.11364927832708263No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT200.11364927832708263No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT190.10796681441072849No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTT190.10796681441072849No Hit
GTGTGGGGCTGCCTGCGGGCTGCGTCTAGTTGCAGTAGTTCTC190.10796681441072849No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT190.10796681441072849No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC190.10796681441072849No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA190.10796681441072849No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC180.10228435049437436No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC180.10228435049437436No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGGAC200.001792997237.03
GGTATCA251.1848032E-436.9999961
TACTGGT250.005351957429.5999982
GTATCAA400.001856860123.1252
GCTGTCT500.006769175618.49999836
GTCTCTT800.0059788613.87537
CTGTCTC1752.845627E-813.74285837
Produced by FastQC (version 0.11.5)