##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632563.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17598
Sequences flagged as poor quality	0
Sequence length	43
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.01426298443005	34.0	33.0	34.0	31.0	34.0
2	33.16825775656324	34.0	33.0	34.0	31.0	34.0
3	33.218661211501306	34.0	34.0	34.0	31.0	34.0
4	36.509660188657804	37.0	37.0	37.0	35.0	37.0
5	36.48857824752813	37.0	37.0	37.0	35.0	37.0
6	36.50948971474031	37.0	37.0	37.0	35.0	37.0
7	36.528014547107624	37.0	37.0	37.0	35.0	37.0
8	36.50744402773042	37.0	37.0	37.0	35.0	37.0
9	38.290317081486535	39.0	39.0	39.0	37.0	39.0
10	38.31469485168769	39.0	39.0	39.0	37.0	39.0
11	38.40379588589612	39.0	39.0	39.0	37.0	39.0
12	38.33685646096147	39.0	39.0	39.0	37.0	39.0
13	38.38174792590067	39.0	39.0	39.0	37.0	39.0
14	39.959199909080574	41.0	40.0	41.0	38.0	41.0
15	39.90964882372997	41.0	40.0	41.0	38.0	41.0
16	39.89896579156722	41.0	40.0	41.0	38.0	41.0
17	39.924309580634166	41.0	40.0	41.0	38.0	41.0
18	39.890896692806	41.0	40.0	41.0	38.0	41.0
19	39.88407773610638	41.0	40.0	41.0	38.0	41.0
20	39.85998408910103	41.0	40.0	41.0	38.0	41.0
21	39.81827480395499	41.0	40.0	41.0	38.0	41.0
22	39.808387316740536	41.0	40.0	41.0	38.0	41.0
23	39.73371974087964	41.0	40.0	41.0	38.0	41.0
24	39.75048300943289	41.0	40.0	41.0	37.0	41.0
25	39.655529037390615	41.0	40.0	41.0	37.0	41.0
26	39.57222411637686	41.0	40.0	41.0	37.0	41.0
27	39.48795317649733	41.0	39.0	41.0	37.0	41.0
28	39.444198204341404	41.0	39.0	41.0	36.0	41.0
29	39.397829298783954	41.0	39.0	41.0	36.0	41.0
30	39.33844755085805	41.0	39.0	41.0	36.0	41.0
31	39.29617001932038	41.0	39.0	41.0	36.0	41.0
32	39.23496988294124	41.0	39.0	41.0	35.0	41.0
33	39.14473235594954	41.0	39.0	41.0	35.0	41.0
34	39.087509944311854	41.0	39.0	41.0	35.0	41.0
35	38.968973747016705	41.0	39.0	41.0	35.0	41.0
36	38.93306057506535	40.0	39.0	41.0	35.0	41.0
37	38.87112171837709	40.0	38.0	41.0	35.0	41.0
38	38.76309807932719	40.0	38.0	41.0	35.0	41.0
39	38.66757586089329	40.0	38.0	41.0	35.0	41.0
40	38.57495169905671	40.0	38.0	41.0	35.0	41.0
41	38.54358449823844	40.0	38.0	41.0	35.0	41.0
42	38.461984316399594	40.0	38.0	41.0	35.0	41.0
43	37.68092965109672	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	1.0
22	5.0
23	7.0
24	13.0
25	14.0
26	18.0
27	33.0
28	42.0
29	58.0
30	97.0
31	113.0
32	154.0
33	184.0
34	321.0
35	502.0
36	717.0
37	1376.0
38	3436.0
39	10503.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.82895783611774	14.751676326855327	11.359245368791909	29.060120468235027
2	22.297988407773612	18.30889873849301	29.94658483918627	29.44652801454711
3	22.002500284123197	18.746448460052278	27.46903057165587	31.782020684168653
4	16.638254347084896	14.598249801113763	32.24230026139334	36.521195590408
5	18.42254801682009	31.730878508921467	32.37299693146948	17.473576542788955
6	37.066712126378	31.588816911012614	14.336856460961473	17.007614501647915
7	32.22525286964428	25.684736901920672	18.40550062507103	23.684509603364017
8	29.060120468235027	29.656779179452208	18.791908171383113	22.49119218092965
9	28.105466530287533	12.28548698715763	16.24048187294011	43.36856460961473
10	20.650073872030912	21.1842254801682	28.452096829185137	29.713603818615752
11	41.19786339356745	17.75769973860666	17.752017274690306	23.292419593135584
12	24.74713035572224	21.655869985225593	25.01988862370724	28.577111035344927
13	36.23707239459029	15.973406068871462	19.394249346516652	28.395272190021593
14	25.2471871803614	17.877031480850096	20.74667575860893	36.12910558017957
15	30.24775542675304	22.320718263439026	18.8430503466303	28.588475963177633
16	27.90658029321514	22.229798840777363	20.348903284464143	29.51471758154336
17	28.582793499261278	22.275258552108195	19.888623707239457	29.253324241391066
18	27.349698829412432	19.752244573246962	22.235481304693717	30.662575292646892
19	29.901125127855437	21.41720650073872	20.490964882372996	28.190703489032842
20	29.8443004886919	20.167064439140812	20.678486191612684	29.31014888055461
21	29.418115694965337	20.45118763495852	20.115922263893623	30.01477440618252
22	30.827366746221163	21.394476645073304	18.877145130128422	28.90101147857711
23	30.406864416410954	21.20695533583362	19.581770655756337	28.804409591999093
24	30.321627457665645	19.996590521650187	19.882941243323103	29.798840777361065
25	29.63973178770315	20.939879531764973	21.070576201841117	28.34981247869076
26	30.725082395726787	21.138765768837366	20.059097624730082	28.07705421070576
27	29.457892942379814	20.411410387544038	22.20138652119559	27.929310148880553
28	27.3667462211615	21.354699397658823	21.07625866575747	30.202295715422206
29	28.56006364359586	21.95135810887601	20.78645300602341	28.702125241504717
30	28.480509148766902	20.485282418456645	22.5593817479259	28.47482668485055
31	28.72485509717013	21.110353449255598	20.627344016365495	29.537447437208776
32	26.991703602682122	20.4284577792931	22.263893624275486	30.315944993749287
33	27.963404932378676	20.365950676213206	23.03670871689965	28.633935674508464
34	28.07137174678941	20.973974315263096	22.11614956245028	28.838504375497216
35	27.389476076826913	21.8036140470508	22.91737697465621	27.889532901466076
36	28.287305375610867	20.38299806796227	22.97420161381975	28.355494942607113
37	27.702011592226388	20.667121263779975	23.06512103648142	28.56574610751222
38	27.667916808728265	20.0818274803955	24.38913512899193	27.861120581884308
39	26.213206046141607	19.42266166609842	25.13353790203432	29.23059438572565
40	26.23025343789067	19.456756449596547	26.95192635526764	27.36106375724514
41	24.678940788725992	19.081713831117174	27.48039549948858	28.758949880668254
42	22.502557108762357	19.445391521763835	29.10558017956586	28.946471189907946
43	22.360495510853507	19.826116604159562	28.662347994090236	29.15103989089669
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	1.0
2	0.0
3	0.5
4	1.0
5	1.0
6	0.5
7	0.0
8	0.5
9	1.0
10	1.0
11	1.0
12	1.0
13	1.5
14	2.0
15	4.0
16	6.0
17	6.5
18	7.0
19	7.0
20	7.0
21	7.0
22	5.5
23	4.0
24	5.0
25	6.0
26	6.0
27	10.5
28	15.0
29	27.5
30	40.0
31	50.0
32	60.0
33	60.0
34	93.5
35	127.0
36	136.5
37	146.0
38	224.5
39	303.0
40	303.0
41	365.0
42	427.0
43	490.5
44	554.0
45	734.5
46	915.0
47	915.0
48	1024.5
49	1134.0
50	1380.5
51	1627.0
52	1761.5
53	1896.0
54	1896.0
55	1863.0
56	1830.0
57	1758.0
58	1686.0
59	1623.0
60	1560.0
61	1560.0
62	1551.0
63	1542.0
64	1284.0
65	1026.0
66	895.0
67	764.0
68	764.0
69	645.5
70	527.0
71	473.0
72	419.0
73	400.0
74	381.0
75	381.0
76	364.0
77	347.0
78	235.0
79	123.0
80	90.0
81	57.0
82	57.0
83	42.0
84	27.0
85	19.5
86	12.0
87	10.5
88	9.0
89	9.0
90	8.0
91	7.0
92	3.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	17598.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.05955222184339
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.55466891736361	64.842595749517
2	6.475868948054937	9.591999090805773
3	2.117701219980051	4.7050801227412204
4	1.0051407964398067	2.977611092169565
5	0.7979743727461062	2.954881236504148
6	0.552443796516535	2.4548244118649847
7	0.40666001688022707	2.1081941129673827
8	0.23785774572239698	1.4092510512558245
9	0.22251208470804879	1.4831230821684283
>10	0.6214992710811018	7.142857142857142
>50	0.007672830507174097	0.3295829071485396
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	58	0.3295829071485396	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	43	0.24434594840322763	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	41	0.23298102057051936	No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT	39	0.2216160927378111	RNA PCR Primer, Index 17 (95% over 21bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	29	0.1647914535742698	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT	28	0.15910898965791567	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	25	0.14206159790885328	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	24	0.13637913399249915	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	23	0.130696670076145	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	23	0.130696670076145	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	22	0.1250142061597909	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	21	0.11933174224343676	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	20	0.11364927832708263	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	20	0.11364927832708263	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	20	0.11364927832708263	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	19	0.10796681441072849	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTT	19	0.10796681441072849	No Hit
GTGTGGGGCTGCCTGCGGGCTGCGTCTAGTTGCAGTAGTTCTC	19	0.10796681441072849	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	19	0.10796681441072849	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	19	0.10796681441072849	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	19	0.10796681441072849	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	18	0.10228435049437436	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	18	0.10228435049437436	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.005682463916354131	0.0
23	0.0	0.0	0.0	0.005682463916354131	0.0
24	0.0	0.0	0.0	0.011364927832708262	0.0
25	0.0	0.0	0.0	0.011364927832708262	0.0
26	0.0	0.0	0.0	0.017047391749062394	0.0
27	0.0	0.0	0.0	0.022729855665416524	0.0
28	0.0	0.0	0.0	0.1477440618252074	0.0
29	0.0	0.0	0.0	0.30117058756676895	0.0
30	0.0	0.0	0.0	0.5341516081372883	0.0
31	0.0	0.0	0.0	0.9830662575292647	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAGGAC	20	0.0017929972	37.0	3
GGTATCA	25	1.1848032E-4	36.999996	1
TACTGGT	25	0.0053519574	29.599998	2
GTATCAA	40	0.0018568601	23.125	2
GCTGTCT	50	0.0067691756	18.499998	36
GTCTCTT	80	0.00597886	13.875	37
CTGTCTC	175	2.845627E-8	13.742858	37
>>END_MODULE