FastQCFastQC Report
Fri 10 Feb 2017
ERR1632562.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632562.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72049
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4050.5621174478479923No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3170.43997834806867553No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2650.3678052436536246No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2510.3483740232341878No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2400.33310663576177324No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2220.30812363807964027No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2180.30257186081694404No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2080.2886924176602035No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2060.28591652902885534No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2020.28036475176615916No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1950.27064914155644076No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1910.26509736429374453No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1890.26232147566239644No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT1850.2567696983997002TruSeq Adapter, Index 11 (95% over 23bp)
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1740.2415023109272856No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1730.24011436661161156No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1700.2359505336645894No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1680.23317464503324128No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1620.22484697913919693No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1600.22207109050784882No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1580.2192952018765007No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1530.21235548029813045No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1470.20402781440408613No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1460.20263987008841208No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1380.1915363155630196No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC1360.1887604269316715No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1320.1832086496689753No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1310.18182070535330122No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1300.18043276103762718No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA1290.17904481672195313No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1280.17765687240627906No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1240.17210509514358283No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC1200.16655331788088662No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT1200.16655331788088662No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1190.16516537356521258No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1180.1637774292495385No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT1160.1610015406181904No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1160.1610015406181904No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1150.15961359630251634No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC1120.15544976335549418No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC1110.15406181903982014No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC1110.15406181903982014No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG1080.14989798609279797No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA1070.1485100417771239No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT1070.1485100417771239No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG1050.14573415314577579No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT1010.14018237588307958No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG1010.14018237588307958No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1000.1387944315674055No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC1000.1387944315674055No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT990.13740648725173146No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA960.1332426543047093No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG960.1332426543047093No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT950.13185470998903526No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC950.13185470998903526No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA930.1290788213576871No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT910.12630293272633902No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA910.12630293272633902No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA910.12630293272633902No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC900.12491498841066496No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA890.1235270440949909No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG880.12213909977931686No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG880.12213909977931686No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA870.1207511554636428No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG870.1207511554636428No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA860.11936321114796875No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA850.1179752668322947No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG840.11658732251662064No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG840.11658732251662064No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA840.11658732251662064No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC840.11658732251662064No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG840.11658732251662064No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA830.11519937820094657No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC820.11381143388527251No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC810.11242348956959847No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC800.11103554525392441No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG800.11103554525392441No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT780.1082596566225763No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC770.10687171230690225No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC750.10409582367555414No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC740.10270787935988009No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG740.10270787935988009No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT730.10131993504420604No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTAT200.001830502337.026
GCCGTCT352.3575834E-531.71428536
CAGTTTC250.005462615329.59999828
GGGCAGT250.005462615329.5999987
CGCCTTA250.005462615329.59999825
AGTAGTT250.005462615329.5999985
CAGTAGT250.005462615329.5999984
TAGTTAT250.005462615329.5999987
GCAGTTT250.005462615329.59999827
AGTCGGT250.005462615329.59999811
GTGATTC250.005462615329.59999816
AGGCAGT250.005462615329.59999825
TGGGCAG250.005462615329.5999986
AATATTC250.005462615329.5999985
CAGGCAG250.005462615329.59999824
ACGGCTG250.005462615329.59999831
GGCAGTT250.005462615329.59999826
ATATTCC250.005462615329.5999986
AGTTTCC250.005462615329.59999829
GTAGTTA250.005462615329.5999986
Produced by FastQC (version 0.11.5)