FastQCFastQC Report
Fri 10 Feb 2017
ERR1632561.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632561.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193913
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9290.47908082490601456No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA7090.3656278846699293No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6480.3341704785135602No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5960.3073543290032128No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5760.2970404253453868No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5080.26197315290877865No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4600.23721978412999645No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4560.23515700339843126No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4490.2315471371181922No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4360.22484309974060532No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4350.22432740455771402No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4350.22432740455771402No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4350.22432740455771402No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4190.2160762816314533No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4110.21195072016832292No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC4100.21143502498543162No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3690.19029152248688844No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT3660.18874443693821455No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC3660.18874443693821455No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC3510.1810090091948451No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3510.1810090091948451No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3500.18049331401195381No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3420.17636775254882345No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT3410.17585205736593215No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3380.17430497181725826No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC3340.17224219108569308No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3270.168632324805454No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC3270.168632324805454No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC3260.16811662962256269No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA3170.16347537297654102No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT3130.16141259224497584No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3070.15831842114762806No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2960.1526457741358238No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2940.15161438377004122No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2930.15109868858714992No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC2900.149551603038476No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA2860.14748882230691082No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG2830.14594173675823693No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC2800.14439465120956305No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG2750.14181617529510657No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2710.13975339456354138No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT2660.1371749186490849No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC2650.1366592234661936No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA2600.13408074755173713No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT2520.12995518608860673No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC2480.12789240535704155No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2440.12582962462547637No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC2440.12582962462547637No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA2400.12376684389391118No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC2390.12325114871101989No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2370.1222197583452373No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT2360.12170406316234601No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG2310.11912558724788952No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC2290.11809419688210691No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC2270.11706280651632432No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG2260.11654711133343304No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2220.11448433060186784No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG2220.11448433060186784No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA2210.11396863541897655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2200.11345294023608525No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG2170.11190585468741138No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG2160.11139015950452007No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT2160.11139015950452007No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG2130.10984307395584618No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC2130.10984307395584618No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC2120.10932737877295487No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC2060.1062332076756071No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT2040.1052018173098245No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA2010.10365473176115061No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT2000.10313903657825933No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC1980.10210764621247674No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT1980.10210764621247674TruSeq Adapter, Index 11 (95% over 21bp)
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT1970.10159195102958543No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA1950.10056056066380284No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG1940.10004486548091154No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAATTC303.5885486E-430.83333416
TTAATGT250.005485221329.635
TAACACC250.005485221329.635
TACTAGC250.005485221329.63
AACACCT250.005485221329.636
TGGGCAG453.9857077E-628.7777776
GGTATCA4200.028.1904751
CAGGTGT358.8427204E-426.4285723
TAGAGGG358.8427204E-426.428575
TAGAATT358.8427204E-426.4285715
GTAGAAT358.8427204E-426.4285714
AATTCCA358.8427204E-426.4285718
TGTAGCG358.8427204E-426.4285727
GGGTCCA601.3278495E-624.66666819
GCAGTCG551.8921044E-523.5454549
AGTCGGT551.8921044E-523.54545411
TGAAATA400.00192543423.12521
GTAGAGG400.00192543423.1254
TCGTAGA400.00192543423.1252
GAATTCC400.00192543423.12517
Produced by FastQC (version 0.11.5)