FastQCFastQC Report
Fri 10 Feb 2017
ERR1632560.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632560.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2714787
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA43600.16060191830887652No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA38150.14052667852026696No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA35420.13047064097477998No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA34390.1266766048312446No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC32430.11945688556781803No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA29840.10991654225543293No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG29340.10807477713721186No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA27180.10011835182649688No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31100.029.0884231
CTCTATG8100.020.0987641
CTTATAC36700.019.96185337
TCTATGG8400.019.8214282
GCGGTAA20850.019.25419623
GTATCAA47100.019.0891722
TAATACG20400.018.77205827
ATACGGC4350.018.71264529
TATAGAG801.617992E-518.55
GTTATAC500.007037290418.53
TTTTACG1601.8189894E-1218.54
CGGTAAT22400.017.92187524
TAAGGTA2800.017.8392875
ATTAGAC1156.414484E-817.6956523
TATTAGA5750.017.6956522
AACTCCG22600.017.51775
GGTAATA23250.017.42580625
CGTGCCA23850.017.37526110
GTAATAC22850.017.32603826
AATACGG22150.017.28893928
Produced by FastQC (version 0.11.5)