##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632560.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2714787
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.062201564984655	34.0	33.0	34.0	31.0	34.0
2	33.20441272188205	34.0	33.0	34.0	31.0	34.0
3	33.278230667820345	34.0	34.0	34.0	31.0	34.0
4	36.56045280900491	37.0	37.0	37.0	35.0	37.0
5	36.528475346316306	37.0	37.0	37.0	35.0	37.0
6	36.56954965527682	37.0	37.0	37.0	35.0	37.0
7	36.56541047235013	37.0	37.0	37.0	35.0	37.0
8	36.54269156291083	37.0	37.0	37.0	35.0	37.0
9	38.33337679899012	39.0	39.0	39.0	37.0	39.0
10	38.36907462721753	39.0	39.0	39.0	37.0	39.0
11	38.434253958045325	39.0	39.0	39.0	37.0	39.0
12	38.39938529247414	39.0	39.0	39.0	37.0	39.0
13	38.42877470681862	39.0	39.0	39.0	37.0	39.0
14	39.99285726651851	41.0	40.0	41.0	38.0	41.0
15	39.98233415733905	41.0	40.0	41.0	38.0	41.0
16	39.95410063478277	41.0	40.0	41.0	38.0	41.0
17	39.94868510862915	41.0	40.0	41.0	38.0	41.0
18	39.94770639464532	41.0	40.0	41.0	38.0	41.0
19	39.95364387703345	41.0	40.0	41.0	38.0	41.0
20	39.923957938504934	41.0	40.0	41.0	38.0	41.0
21	39.8939942617966	41.0	40.0	41.0	38.0	41.0
22	39.86885711475707	41.0	40.0	41.0	38.0	41.0
23	39.83127295069558	41.0	40.0	41.0	38.0	41.0
24	39.812560617094455	41.0	40.0	41.0	38.0	41.0
25	39.76856968889272	41.0	40.0	41.0	38.0	41.0
26	39.697146774314156	41.0	40.0	41.0	38.0	41.0
27	39.63259695880377	41.0	40.0	41.0	37.0	41.0
28	39.58555496250719	41.0	40.0	41.0	37.0	41.0
29	39.55409356240471	41.0	40.0	41.0	37.0	41.0
30	39.49691080736721	41.0	40.0	41.0	37.0	41.0
31	39.44415381390879	41.0	39.0	41.0	37.0	41.0
32	39.395612989159005	41.0	39.0	41.0	36.0	41.0
33	39.33315099858663	41.0	39.0	41.0	36.0	41.0
34	39.297329035390256	41.0	39.0	41.0	36.0	41.0
35	39.22174594176265	41.0	39.0	41.0	35.0	41.0
36	39.18066831762492	41.0	39.0	41.0	35.0	41.0
37	39.12670496801407	41.0	39.0	41.0	35.0	41.0
38	39.05306862011642	41.0	39.0	41.0	35.0	41.0
39	38.97560361089102	41.0	39.0	41.0	35.0	41.0
40	38.904098921941205	40.0	39.0	41.0	35.0	41.0
41	38.853464746958046	40.0	39.0	41.0	35.0	41.0
42	38.789367268960696	40.0	38.0	41.0	35.0	41.0
43	38.07890932143111	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	3.0
13	1.0
14	2.0
15	5.0
16	7.0
17	14.0
18	44.0
19	96.0
20	186.0
21	326.0
22	574.0
23	1037.0
24	1550.0
25	2305.0
26	3170.0
27	4300.0
28	6131.0
29	8110.0
30	11079.0
31	14800.0
32	19560.0
33	26433.0
34	39508.0
35	62643.0
36	100737.0
37	192304.0
38	503213.0
39	1716646.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.060799981729694	16.41410541600501	12.47803234655242	28.04706225571288
2	20.9846297333824	18.820334707658464	32.5962220977189	27.598813461240233
3	20.894604254403752	19.78000484015873	28.75816776785803	30.567223137579486
4	15.798661184100263	15.347428730136103	33.35226667874864	35.50164340701499
5	17.26632697150826	32.56988485652834	33.42310096519543	16.74068720676797
6	35.483004744018594	33.21708111907122	14.85468289040724	16.44523124650295
7	30.924893923538015	27.44753087442956	19.945726865496262	21.681848336536163
8	27.828481571482406	30.836673374375227	19.763723636513657	21.571121417628714
9	28.27643568353613	12.927054682374713	17.590993326548272	41.20551630754088
10	19.31433294766772	23.349566651085336	30.071640979568564	27.26445942167838
11	39.28963856096261	19.36159264060127	18.748027009117106	22.600741789319017
12	23.41005021756771	23.58273411505212	26.362399702076072	26.64481596530409
13	34.36844216507593	17.660612048017025	21.008609515221636	26.962336271685405
14	24.481847010465277	19.42483885476098	22.861683071268573	33.23163106350517
15	29.32539458896775	23.93056987527935	20.12776692978123	26.616268605971666
16	26.431023870380994	23.67062314649363	22.176251764871424	27.722101218253954
17	26.914818731635297	23.35984370044501	22.017381105773676	27.707956462146015
18	26.594793624693207	21.728223982212967	23.5863808099862	28.090601583107627
19	28.38049541271562	21.999515984126933	23.087262462948292	26.532726140209157
20	29.481023741457435	21.031079049663933	22.791180302543072	26.69671690633556
21	27.632775610020232	22.196363839962398	21.995390430262116	28.175470119755254
22	28.397034463477244	22.845991232461333	21.298319168317807	27.458655135743616
23	28.129831180125738	22.267676985339918	22.38466590564932	27.21782592888503
24	27.856439565977	21.978888214802854	22.203399382714004	27.96127283650614
25	28.061649035449186	21.994948406633743	22.836414053846582	27.106988504070483
26	27.76269372145955	22.63518279702975	22.443860236548947	27.158263244961763
27	27.30398370111541	21.95277198542648	23.380434634466717	27.36280967899139
28	26.54418191924449	22.675996312049527	22.562838263186023	28.216983505519956
29	27.34689682836996	23.134153802858197	22.55937574476377	26.959573624008073
30	27.10371016216005	22.243402521081766	23.5294334325308	27.123453884227384
31	27.46031272435001	22.450195908555624	22.69411928081282	27.39537208628154
32	25.5324635045033	22.286168307126857	23.082473873640915	29.09889431472893
33	26.014784953663035	21.86193612979582	24.542293741645295	27.580985174895854
34	26.487013529974913	21.361344370663335	24.40511907564019	27.746523023721565
35	26.01651621287416	22.62262195892348	24.712583344476013	26.648278483726344
36	26.011764458869145	21.538669516245655	25.146576876933622	27.30298914795157
37	26.118734176935426	21.040766734185777	25.1961940292185	27.644305059660297
38	25.25951391398294	20.35570378081227	26.522854279175494	27.861928026029297
39	24.63983362230628	19.65866935417033	27.647546566268367	28.053950457255027
40	24.260356337348014	20.43976194080788	28.655397274261297	26.644484447582812
41	22.859951812057446	19.689795184668263	29.514507031306692	27.935745971967595
42	21.458810580719593	20.498366906869673	30.339617804269725	27.703204708141005
43	20.917921000800433	20.60986736712678	30.15017384420951	28.322037787863287
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	24.5
2	24.0
3	70.0
4	116.0
5	116.0
6	135.5
7	155.0
8	160.0
9	165.0
10	260.0
11	355.0
12	355.0
13	719.5
14	1084.0
15	1797.0
16	2510.0
17	2545.0
18	2580.0
19	2580.0
20	3188.5
21	3797.0
22	3781.0
23	3765.0
24	4768.0
25	5771.0
26	5771.0
27	7282.5
28	8794.0
29	11793.5
30	14793.0
31	19240.5
32	23688.0
33	23688.0
34	30826.5
35	37965.0
36	44452.0
37	50939.0
38	63047.0
39	75155.0
40	75155.0
41	86682.0
42	98209.0
43	113335.5
44	128462.0
45	156748.0
46	185034.0
47	185034.0
48	206500.0
49	227966.0
50	251044.5
51	274123.0
52	284915.5
53	295708.0
54	295708.0
55	279961.5
56	264215.0
57	251353.0
58	238491.0
59	223181.0
60	207871.0
61	207871.0
62	190855.0
63	173839.0
64	147334.0
65	120829.0
66	105003.0
67	89177.0
68	89177.0
69	76279.5
70	63382.0
71	54692.5
72	46003.0
73	36854.0
74	27705.0
75	27705.0
76	22285.5
77	16866.0
78	14033.0
79	11200.0
80	8790.5
81	6381.0
82	6381.0
83	5017.5
84	3654.0
85	2795.5
86	1937.0
87	1549.0
88	1161.0
89	1161.0
90	860.0
91	559.0
92	386.0
93	213.0
94	153.0
95	93.0
96	93.0
97	57.0
98	21.0
99	14.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2714787.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.07720413463689
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.5071719463614	36.80762664585993
2	8.776701354875305	7.737049144951659
3	2.498819629614367	3.304229487304506
4	1.2382374429886216	2.1831217816704114
5	0.7342032390515234	1.61808130219928
6	0.47501941729491987	1.2562516694414667
7	0.35887120777031106	1.1072627638054982
8	0.26973280990793397	0.9511254499296972
9	0.20964052510218234	0.8316331397839225
>10	1.5470176083102698	13.520087319374822
>50	0.19479428145949088	6.031903023654301
>100	0.16547940724748542	14.279010994549557
>500	0.017101622494224707	5.19428996268038
>1k	0.007209507522075049	5.178327314794688
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	4360	0.16060191830887652	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	3815	0.14052667852026696	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	3542	0.13047064097477998	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	3439	0.1266766048312446	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	3243	0.11945688556781803	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	2984	0.10991654225543293	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	2934	0.10807477713721186	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	2718	0.10011835182649688	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.683530236442122E-5
2	0.0	0.0	0.0	0.0	3.683530236442122E-5
3	0.0	0.0	0.0	0.0	3.683530236442122E-5
4	0.0	0.0	0.0	3.683530236442122E-5	3.683530236442122E-5
5	7.367060472884245E-5	3.683530236442122E-5	0.0	3.683530236442122E-5	3.683530236442122E-5
6	7.367060472884245E-5	3.683530236442122E-5	0.0	3.683530236442122E-5	3.683530236442122E-5
7	7.367060472884245E-5	3.683530236442122E-5	0.0	3.683530236442122E-5	3.683530236442122E-5
8	7.367060472884245E-5	3.683530236442122E-5	0.0	3.683530236442122E-5	3.683530236442122E-5
9	7.367060472884245E-5	3.683530236442122E-5	0.0	3.683530236442122E-5	3.683530236442122E-5
10	1.473412094576849E-4	3.683530236442122E-5	0.0	3.683530236442122E-5	3.683530236442122E-5
11	1.473412094576849E-4	3.683530236442122E-5	0.0	7.367060472884245E-5	3.683530236442122E-5
12	1.841765118221061E-4	3.683530236442122E-5	0.0	1.473412094576849E-4	3.683530236442122E-5
13	1.841765118221061E-4	3.683530236442122E-5	0.0	1.473412094576849E-4	3.683530236442122E-5
14	1.841765118221061E-4	3.683530236442122E-5	0.0	2.946824189153698E-4	3.683530236442122E-5
15	2.2101181418652735E-4	3.683530236442122E-5	0.0	4.788589307374759E-4	3.683530236442122E-5
16	2.2101181418652735E-4	3.683530236442122E-5	0.0	0.0010313884662037942	3.683530236442122E-5
17	2.2101181418652735E-4	3.683530236442122E-5	0.0	0.0015839180016701125	3.683530236442122E-5
18	2.2101181418652735E-4	3.683530236442122E-5	0.0	0.0018417651182210612	7.367060472884245E-5
19	2.2101181418652735E-4	7.367060472884245E-5	0.0	0.0022469534442296946	7.367060472884245E-5
20	2.2101181418652735E-4	7.367060472884245E-5	0.0	0.0033151772127979103	7.367060472884245E-5
21	2.2101181418652735E-4	7.367060472884245E-5	0.0	0.004935930516832444	7.367060472884245E-5
22	2.2101181418652735E-4	7.367060472884245E-5	0.0	0.007919590008350562	1.1050590709326368E-4
23	2.2101181418652735E-4	7.367060472884245E-5	0.0	0.00961401391711394	1.473412094576849E-4
24	2.2101181418652735E-4	7.367060472884245E-5	0.0	0.012450332199174373	1.473412094576849E-4
25	2.2101181418652735E-4	7.367060472884245E-5	0.0	0.01414475610793775	1.473412094576849E-4
26	2.2101181418652735E-4	7.367060472884245E-5	0.0	0.017680945134922188	1.473412094576849E-4
27	2.2101181418652735E-4	7.367060472884245E-5	0.0	0.04670716339808611	1.473412094576849E-4
28	2.2101181418652735E-4	7.367060472884245E-5	0.0	0.1770304631634084	1.473412094576849E-4
29	2.2101181418652735E-4	7.367060472884245E-5	0.0	0.37601476653601185	1.473412094576849E-4
30	2.2101181418652735E-4	7.367060472884245E-5	0.0	0.6255739400549657	1.473412094576849E-4
31	2.2101181418652735E-4	7.367060472884245E-5	0.0	1.3379318524805077	2.2101181418652735E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3110	0.0	29.088423	1
CTCTATG	810	0.0	20.098764	1
CTTATAC	3670	0.0	19.961853	37
TCTATGG	840	0.0	19.821428	2
GCGGTAA	2085	0.0	19.254196	23
GTATCAA	4710	0.0	19.089172	2
TAATACG	2040	0.0	18.772058	27
ATACGGC	435	0.0	18.712645	29
TATAGAG	80	1.617992E-5	18.5	5
GTTATAC	50	0.0070372904	18.5	3
TTTTACG	160	1.8189894E-12	18.5	4
CGGTAAT	2240	0.0	17.921875	24
TAAGGTA	280	0.0	17.839287	5
ATTAGAC	115	6.414484E-8	17.695652	3
TATTAGA	575	0.0	17.695652	2
AACTCCG	2260	0.0	17.5177	5
GGTAATA	2325	0.0	17.425806	25
CGTGCCA	2385	0.0	17.375261	10
GTAATAC	2285	0.0	17.326038	26
AATACGG	2215	0.0	17.288939	28
>>END_MODULE