FastQCFastQC Report
Fri 10 Feb 2017
ERR1632559.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632559.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2369230
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA32880.13877926583742398No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA25460.10746107385099801No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA24300.10256496836524946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23800.029.7710081
TATACCG400.001931788523.1250025
CTATATA450.00382698920.5555552
GTATCAA35300.019.9150142
TATACAC8250.019.5090937
CTTATAC29400.018.68877637
GAATCGT500.007037031518.56
ATACGGC2800.017.83928529
GCGGTAA15300.017.5326823
TATGAGT852.7250911E-517.4117644
TCTTATA54400.017.3437537
AACTCCG15750.016.9142865
ATAATTC1005.8830883E-616.6500023
GGTAATA16550.016.54380625
TAATACG15050.016.47176227
CGGTAAT16350.016.40672924
TAAGGTA2400.016.18755
AAGACCG1151.2434793E-616.0869565
GGACCGT3800.016.065796
AGCCGCG17150.015.85714219
Produced by FastQC (version 0.11.5)