##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632559.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2369230
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.07006960067195	34.0	33.0	34.0	31.0	34.0
2	33.20241597481038	34.0	33.0	34.0	31.0	34.0
3	33.276044537676796	34.0	34.0	34.0	31.0	34.0
4	36.556370213107215	37.0	37.0	37.0	35.0	37.0
5	36.52720799584675	37.0	37.0	37.0	35.0	37.0
6	36.57029583451163	37.0	37.0	37.0	35.0	37.0
7	36.56547401476429	37.0	37.0	37.0	35.0	37.0
8	36.53995095452953	37.0	37.0	37.0	35.0	37.0
9	38.33490754380115	39.0	39.0	39.0	37.0	39.0
10	38.36502661202163	39.0	39.0	39.0	37.0	39.0
11	38.43881429831633	39.0	39.0	39.0	37.0	39.0
12	38.40013548705698	39.0	39.0	39.0	37.0	39.0
13	38.430287899444124	39.0	39.0	39.0	37.0	39.0
14	39.999478311518935	41.0	40.0	41.0	38.0	41.0
15	39.993433731634326	41.0	40.0	41.0	38.0	41.0
16	39.965346125112376	41.0	40.0	41.0	38.0	41.0
17	39.96129966275963	41.0	40.0	41.0	38.0	41.0
18	39.95202998442532	41.0	40.0	41.0	38.0	41.0
19	39.96186946813944	41.0	40.0	41.0	38.0	41.0
20	39.92974679537234	41.0	40.0	41.0	38.0	41.0
21	39.90142282513728	41.0	40.0	41.0	38.0	41.0
22	39.87587950515568	41.0	40.0	41.0	38.0	41.0
23	39.83701244708196	41.0	40.0	41.0	38.0	41.0
24	39.820172376679345	41.0	40.0	41.0	38.0	41.0
25	39.77503830358386	41.0	40.0	41.0	38.0	41.0
26	39.7008796106752	41.0	40.0	41.0	38.0	41.0
27	39.633849394106946	41.0	40.0	41.0	37.0	41.0
28	39.58366135833161	41.0	40.0	41.0	37.0	41.0
29	39.546769625574555	41.0	40.0	41.0	37.0	41.0
30	39.49725353806933	41.0	40.0	41.0	37.0	41.0
31	39.44320813091173	41.0	40.0	41.0	37.0	41.0
32	39.39102324383872	41.0	39.0	41.0	36.0	41.0
33	39.3290275743596	41.0	39.0	41.0	36.0	41.0
34	39.2941039915922	41.0	39.0	41.0	36.0	41.0
35	39.21960383753371	41.0	39.0	41.0	35.0	41.0
36	39.17564736222317	41.0	39.0	41.0	35.0	41.0
37	39.12463922877897	41.0	39.0	41.0	35.0	41.0
38	39.04906404190391	41.0	39.0	41.0	35.0	41.0
39	38.9743152838686	41.0	39.0	41.0	35.0	41.0
40	38.90510503412501	40.0	39.0	41.0	35.0	41.0
41	38.85541040760078	40.0	39.0	41.0	35.0	41.0
42	38.78872460672877	40.0	38.0	41.0	35.0	41.0
43	38.06853112614647	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	1.0
15	2.0
16	3.0
17	12.0
18	35.0
19	75.0
20	144.0
21	284.0
22	560.0
23	977.0
24	1363.0
25	2114.0
26	2836.0
27	3913.0
28	5344.0
29	7279.0
30	9744.0
31	13117.0
32	17276.0
33	23024.0
34	34878.0
35	53871.0
36	86683.0
37	163874.0
38	437500.0
39	1504319.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.11628672606712	16.445131962705183	12.517780038240273	27.92080127298743
2	20.832000270129956	19.04796072985738	32.71729633678452	27.402742663228135
3	21.019951629854425	19.81512981010708	28.666908658087227	30.498009901951267
4	16.087420807604158	15.058647746314202	32.842906767177524	36.01102467890412
5	17.27949586996619	32.90588081359766	33.11586464800801	16.69875866842814
6	36.14233316309518	32.703452176445516	14.64745930112315	16.506755359336157
7	31.048315275427036	27.54380115058479	19.67348041346767	21.734403160520507
8	27.954398686493082	31.242555598232336	19.62004533118355	21.183000384091034
9	28.065658462876126	13.135322446533262	17.46753164530248	41.33148744528813
10	19.059061382812136	23.788952528880692	30.40557480700481	26.74641128130236
11	39.14634712543738	19.447753067452293	18.834684686585938	22.57121512052439
12	23.50350113750037	23.501517370622523	26.39135921797377	26.603622273903337
13	34.193345517319976	17.733356406933897	21.22440624169034	26.848891834055788
14	24.622472280023466	19.27596729739198	22.637354752387907	33.46420567019665
15	28.99342824461956	24.2475403401105	20.08986041878585	26.669170996484088
16	26.676135284459505	23.620079097428277	21.894750615178772	27.80903500293344
17	26.95120355558557	23.375906940229527	22.07911431140075	27.593775192784154
18	26.419849486964118	21.933033095140615	23.6709817113577	27.97613570653757
19	28.32367478041389	22.116890297691654	23.090962042520143	26.46847287937431
20	29.18505168345834	21.348708230100076	22.978815902212954	26.48742418422863
21	27.563892066198726	22.40162415637148	21.93527010885393	28.099213668575867
22	28.131080562039145	22.839530142704593	21.446883586650515	27.582505708605748
23	27.8295902044124	22.536224849423654	22.39955597388181	27.23462897228213
24	27.976811031432153	22.085529897899317	22.186913047699043	27.750746022969487
25	28.093768861613267	22.270864373657265	22.646935924329846	26.988430840399623
26	27.823681111584776	22.59827032411374	22.285383858890864	27.29266470541062
27	27.449382288760482	21.93239997805194	23.12147828619425	27.496739446993328
28	26.597628765463888	22.712653478134246	22.482578728109974	28.207139028291895
29	27.362687455418005	23.061374370576093	22.4416371563757	27.134301017630204
30	26.99531071276322	22.694968407457274	23.239322480299506	27.07039839948
31	27.543379072525674	22.434208582535252	22.665971644795988	27.356440700143086
32	25.56362193624089	22.46476703401527	23.053481510870622	28.91812951887322
33	26.224976047070147	22.050075340933553	24.148436411830005	27.5765122001663
34	26.39144363358559	21.546916086661067	24.169244860144435	27.8923954196089
35	26.234852673653464	22.595526816729485	24.330520886532756	26.839099623084294
36	26.045846118781206	21.67923755819401	24.899524318027378	27.375392004997405
37	26.400265065021124	21.07617242732871	24.770790509996917	27.752771997653248
38	25.463505020618516	20.66890930808744	26.25907995424674	27.60850571704731
39	24.868417165070507	19.850204496819643	27.262401708571982	28.018976629537867
40	24.43895273991972	20.533506666722946	28.21798643441118	26.809554158946153
41	23.126923093156847	19.92807789872659	29.191425062150994	27.753573945965567
42	22.024244163715636	20.39628908970425	29.81681812234355	27.76264862423657
43	21.347737450564107	20.62400020259747	29.778915512634907	28.24934683420352
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19.0
1	19.0
2	19.0
3	56.5
4	94.0
5	94.0
6	105.5
7	117.0
8	127.5
9	138.0
10	209.5
11	281.0
12	281.0
13	557.5
14	834.0
15	1463.5
16	2093.0
17	2132.0
18	2171.0
19	2171.0
20	2737.0
21	3303.0
22	3461.5
23	3620.0
24	4725.0
25	5830.0
26	5830.0
27	7286.0
28	8742.0
29	12688.0
30	16634.0
31	19815.0
32	22996.0
33	22996.0
34	28231.0
35	33466.0
36	39053.0
37	44640.0
38	55737.0
39	66834.0
40	66834.0
41	77682.0
42	88530.0
43	98566.0
44	108602.0
45	131034.0
46	153466.0
47	153466.0
48	171513.0
49	189560.0
50	210847.0
51	232134.0
52	245282.0
53	258430.0
54	258430.0
55	244820.0
56	231210.0
57	221274.0
58	211338.0
59	196334.0
60	181330.0
61	181330.0
62	167217.5
63	153105.0
64	131304.0
65	109503.0
66	94763.0
67	80023.0
68	80023.0
69	68259.5
70	56496.0
71	48825.5
72	41155.0
73	33361.0
74	25567.0
75	25567.0
76	20291.5
77	15016.0
78	12224.0
79	9432.0
80	7482.0
81	5532.0
82	5532.0
83	4403.5
84	3275.0
85	2558.5
86	1842.0
87	1437.0
88	1032.0
89	1032.0
90	783.5
91	535.0
92	365.5
93	196.0
94	129.5
95	63.0
96	63.0
97	42.5
98	22.0
99	13.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2369230.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.98770755404097
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.33570816224452	40.95065007206777
2	7.844599487543522	7.528886921736364
3	2.1937966357268084	3.1582581416489104
4	1.086861278850974	2.0862392480524607
5	0.6671203820885704	1.6006788899503193
6	0.4420874687834771	1.2728858499172628
7	0.30931198068134597	1.0390221010328322
8	0.24397761348985353	0.9366341092707146
9	0.18584526593670078	0.8026459444866034
>10	1.334688865671579	12.672794490169823
>50	0.18395537017856678	6.180961041250048
>100	0.1547931466791861	14.618917709912088
>500	0.012918635642362295	4.1226998502490675
>1k	0.004335706482710632	3.0287256302556895
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	3288	0.13877926583742398	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2546	0.10746107385099801	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	2430	0.10256496836524946	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	4.2207805911625294E-5	0.0	0.0	0.0	0.0
4	4.2207805911625294E-5	0.0	0.0	0.0	0.0
5	4.2207805911625294E-5	0.0	0.0	0.0	0.0
6	4.2207805911625294E-5	0.0	0.0	0.0	0.0
7	4.2207805911625294E-5	0.0	0.0	0.0	0.0
8	8.441561182325059E-5	0.0	0.0	0.0	0.0
9	8.441561182325059E-5	0.0	0.0	0.0	0.0
10	8.441561182325059E-5	0.0	0.0	0.0	0.0
11	8.441561182325059E-5	0.0	0.0	0.0	0.0
12	1.2662341773487588E-4	0.0	0.0	0.0	0.0
13	1.2662341773487588E-4	0.0	0.0	0.0	0.0
14	1.2662341773487588E-4	0.0	0.0	4.2207805911625294E-5	0.0
15	2.1103902955812648E-4	0.0	0.0	4.2207805911625294E-5	4.2207805911625294E-5
16	2.1103902955812648E-4	0.0	0.0	7.597405064092553E-4	4.2207805911625294E-5
17	2.5324683546975175E-4	0.0	0.0	0.0013084419832603842	4.2207805911625294E-5
18	2.5324683546975175E-4	0.0	4.2207805911625294E-5	0.0015194810128185106	4.2207805911625294E-5
19	3.3766244729300235E-4	0.0	4.2207805911625294E-5	0.001983766877846389	4.2207805911625294E-5
20	3.3766244729300235E-4	0.0	4.2207805911625294E-5	0.0026168839665207683	4.2207805911625294E-5
21	3.3766244729300235E-4	0.0	4.2207805911625294E-5	0.004009741561604403	4.2207805911625294E-5
22	3.7987025320462765E-4	0.0	4.2207805911625294E-5	0.006711041139948422	4.2207805911625294E-5
23	3.7987025320462765E-4	0.0	4.2207805911625294E-5	0.008146106540943682	4.2207805911625294E-5
24	3.7987025320462765E-4	0.0	4.2207805911625294E-5	0.010636367089729575	4.2207805911625294E-5
25	3.7987025320462765E-4	0.0	4.2207805911625294E-5	0.01139610759613883	4.2207805911625294E-5
26	3.7987025320462765E-4	0.0	4.2207805911625294E-5	0.013801952533101472	4.2207805911625294E-5
27	3.7987025320462765E-4	0.0	4.2207805911625294E-5	0.03486364768300249	4.2207805911625294E-5
28	3.7987025320462765E-4	0.0	4.2207805911625294E-5	0.13675329115366597	4.2207805911625294E-5
29	3.7987025320462765E-4	0.0	4.2207805911625294E-5	0.29975983758436286	4.2207805911625294E-5
30	3.7987025320462765E-4	0.0	4.2207805911625294E-5	0.5191982205189027	4.2207805911625294E-5
31	3.7987025320462765E-4	0.0	4.2207805911625294E-5	1.143789332399134	4.2207805911625294E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2380	0.0	29.771008	1
TATACCG	40	0.0019317885	23.125002	5
CTATATA	45	0.003826989	20.555555	2
GTATCAA	3530	0.0	19.915014	2
TATACAC	825	0.0	19.50909	37
CTTATAC	2940	0.0	18.688776	37
GAATCGT	50	0.0070370315	18.5	6
ATACGGC	280	0.0	17.839285	29
GCGGTAA	1530	0.0	17.53268	23
TATGAGT	85	2.7250911E-5	17.411764	4
TCTTATA	5440	0.0	17.34375	37
AACTCCG	1575	0.0	16.914286	5
ATAATTC	100	5.8830883E-6	16.650002	3
GGTAATA	1655	0.0	16.543806	25
TAATACG	1505	0.0	16.471762	27
CGGTAAT	1635	0.0	16.406729	24
TAAGGTA	240	0.0	16.1875	5
AAGACCG	115	1.2434793E-6	16.086956	5
GGACCGT	380	0.0	16.06579	6
AGCCGCG	1715	0.0	15.857142	19
>>END_MODULE