FastQCFastQC Report
Fri 10 Feb 2017
ERR1632558.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632558.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1322550
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA26580.20097538845412272No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA21660.1637745264829307No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA16470.12453215379380743No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA15680.11855884465615665No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA14610.1104684132925031No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT13470.10184870137234887No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATAGA250.005496638429.64
GGTATCA15000.029.3533331
TTTTACG551.9029396E-523.5454544
ATACACA3150.021.73015837
CTAGATA603.7268328E-521.5833343
TTACGAG603.7268328E-521.5833346
GTATCAA20950.020.92842
TATTAGA2050.018.9512212
ATACGGC1501.2732926E-1118.529
GCGGTAA12650.018.28063223
GGTAATA12850.017.99610925
CTTATAC17100.017.95906437
TAATACG11900.017.72268927
AACTCCG12900.017.6395345
CGTGCCA13750.017.49090810
CGGTAAT13250.017.31320824
TATACAC5800.017.22413837
TCTTATA31200.017.19551337
TAGATAA650.001580104217.0769234
TAACTCC13450.017.0557614
Produced by FastQC (version 0.11.5)