##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632558.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1322550
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.0571653245624	34.0	33.0	34.0	31.0	34.0
2	33.18937733923103	34.0	33.0	34.0	31.0	34.0
3	33.26387282144342	34.0	34.0	34.0	31.0	34.0
4	36.54684510982572	37.0	37.0	37.0	35.0	37.0
5	36.51665645911308	37.0	37.0	37.0	35.0	37.0
6	36.55867982306907	37.0	37.0	37.0	35.0	37.0
7	36.55479339155419	37.0	37.0	37.0	35.0	37.0
8	36.52820762920116	37.0	37.0	37.0	35.0	37.0
9	38.31990548561491	39.0	39.0	39.0	37.0	39.0
10	38.35489773543533	39.0	39.0	39.0	37.0	39.0
11	38.425924161657406	39.0	39.0	39.0	37.0	39.0
12	38.38692223356395	39.0	39.0	39.0	37.0	39.0
13	38.419278666212996	39.0	39.0	39.0	37.0	39.0
14	39.98272957544138	41.0	40.0	41.0	38.0	41.0
15	39.97453555631167	41.0	40.0	41.0	38.0	41.0
16	39.9383962799138	41.0	40.0	41.0	38.0	41.0
17	39.93662621450985	41.0	40.0	41.0	38.0	41.0
18	39.92784242561718	41.0	40.0	41.0	38.0	41.0
19	39.93404861819969	41.0	40.0	41.0	38.0	41.0
20	39.90147366829231	41.0	40.0	41.0	38.0	41.0
21	39.874022910286946	41.0	40.0	41.0	38.0	41.0
22	39.84124607765302	41.0	40.0	41.0	38.0	41.0
23	39.80501757967563	41.0	40.0	41.0	38.0	41.0
24	39.781958338059056	41.0	40.0	41.0	38.0	41.0
25	39.736567993648634	41.0	40.0	41.0	38.0	41.0
26	39.650354996030394	41.0	40.0	41.0	37.0	41.0
27	39.57474802464935	41.0	40.0	41.0	37.0	41.0
28	39.52950134210427	41.0	40.0	41.0	37.0	41.0
29	39.49316774413066	41.0	40.0	41.0	37.0	41.0
30	39.44045215681827	41.0	39.0	41.0	37.0	41.0
31	39.380671430191676	41.0	39.0	41.0	36.0	41.0
32	39.32759820044611	41.0	39.0	41.0	36.0	41.0
33	39.26166194094741	41.0	39.0	41.0	36.0	41.0
34	39.21684473176818	41.0	39.0	41.0	35.0	41.0
35	39.13993043741257	41.0	39.0	41.0	35.0	41.0
36	39.08847983063022	41.0	39.0	41.0	35.0	41.0
37	39.0334679218177	41.0	39.0	41.0	35.0	41.0
38	38.953550338361495	41.0	39.0	41.0	35.0	41.0
39	38.864763525008506	40.0	39.0	41.0	35.0	41.0
40	38.78963290612831	40.0	38.0	41.0	35.0	41.0
41	38.72931760613965	40.0	38.0	41.0	35.0	41.0
42	38.65303844845185	40.0	38.0	41.0	35.0	41.0
43	37.9142444520056	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	0.0
14	0.0
15	0.0
16	2.0
17	6.0
18	16.0
19	49.0
20	67.0
21	180.0
22	309.0
23	482.0
24	817.0
25	1208.0
26	1691.0
27	2232.0
28	3168.0
29	4365.0
30	5729.0
31	7468.0
32	10162.0
33	13547.0
34	19844.0
35	31650.0
36	50590.0
37	97814.0
38	255845.0
39	815306.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.61801066122264	15.897243960530794	12.28641639257495	28.198328985671615
2	21.225208876791047	18.86862500472572	32.21050243847113	27.6956636800121
3	21.65657253033912	19.36887074212695	28.078031076329818	30.896525651204115
4	16.288987183849382	15.166685569543684	32.43476617141129	36.10956107519564
5	17.937015613776417	32.26040603379835	32.410721711844545	17.3918566405807
6	35.72636195228914	32.37798192884957	14.869607954330649	17.026048164530643
7	31.27231484631961	27.01946996332842	19.345733620657064	22.362481569694907
8	27.939283959018564	30.719670333824805	19.472534119693016	21.868511587463612
9	28.01625647423538	13.185739669577709	17.222713697024687	41.57529015916223
10	19.66753619900949	23.155419454841027	29.55434577142641	27.62269857472307
11	39.49000037805754	19.301727722959434	18.630070696760047	22.578201202222978
12	23.435635703754112	22.95625874258062	26.33609315337794	27.27201240028732
13	34.582208612150765	17.428225775963103	20.940229102869456	27.049336509016676
14	24.767381195417943	19.07239801897849	22.495028543344297	33.66519224225927
15	29.473819515330234	23.62360591281993	19.768099504744622	27.134475067105214
16	26.651771199576572	23.37575138936146	21.848928206873087	28.12354920418888
17	27.31798419719481	23.12131866470077	21.674719292276283	27.885977845828137
18	26.696608823862995	21.543835771804467	23.30807908963744	28.451476314695096
19	28.626970624929115	22.00846848890401	22.840875581263468	26.523685304903406
20	29.525008506294657	20.934709462780233	22.512267967184606	27.028014063740503
21	27.849381875921512	21.918793240331176	21.681826774035006	28.5499981097123
22	28.515216815999395	22.417148690030622	21.142036217912366	27.925598276057617
23	28.364901137953197	22.07961891799932	22.05345733620657	27.502022607840914
24	28.20520963290613	21.6106763449397	22.035310574269403	28.148803447884767
25	28.683679255982756	21.813920078635967	22.34834221768553	27.15405844769574
26	28.35318135420211	22.263052436580846	22.124985822842234	27.258780386374802
27	27.9160712260406	21.57113152621829	23.045631545121168	27.46716570261994
28	26.91671392385921	22.563078900608673	22.055272012400287	28.464935163131827
29	27.576726777815587	22.937733923103096	21.983970360288836	27.501568938792488
30	27.345128728592492	22.298514233866396	23.214245208120676	27.14211182942044
31	27.98268496465162	21.936864390760274	22.339193225208874	27.741257419379227
32	25.914634607387242	21.97837510869154	23.007901402593475	29.099088881327738
33	26.599826093531437	21.641677063249027	23.784053532947716	27.974443310271823
34	26.799742920872553	21.322898945219464	23.782994971834714	28.094363162073265
35	26.576159691505048	22.31953423311028	24.02722014290575	27.077085932478923
36	26.491323579448796	21.06158557332426	24.82204831575366	27.625042531473294
37	26.849646516199766	20.459869192091038	24.613133718952025	28.077350572757176
38	25.75963101584061	20.09058258666969	26.084231219991683	28.065555177498013
39	25.153756001663453	19.474802464935163	27.067332047937697	28.304109485463684
40	24.865298098370573	20.1819212884201	28.00098294960493	26.9517976636044
41	23.35601678575479	19.717288571320555	28.84480737968319	28.081887263241466
42	22.270689198896072	20.433480775774076	29.36894635363502	27.92688367169483
43	21.626630373142792	20.798155079203053	29.158821972704246	28.416392574949906
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8.0
1	8.0
2	8.0
3	25.0
4	42.0
5	42.0
6	65.5
7	89.0
8	86.5
9	84.0
10	131.0
11	178.0
12	178.0
13	377.5
14	577.0
15	1022.0
16	1467.0
17	1413.0
18	1359.0
19	1359.0
20	1705.0
21	2051.0
22	1850.0
23	1649.0
24	1944.5
25	2240.0
26	2240.0
27	2733.5
28	3227.0
29	4579.5
30	5932.0
31	7560.5
32	9189.0
33	9189.0
34	11979.0
35	14769.0
36	16991.5
37	19214.0
38	25272.0
39	31330.0
40	31330.0
41	37184.0
42	43038.0
43	49443.0
44	55848.0
45	70425.0
46	85002.0
47	85002.0
48	96815.5
49	108629.0
50	121706.5
51	134784.0
52	141980.0
53	149176.0
54	149176.0
55	140773.5
56	132371.0
57	125922.5
58	119474.0
59	111507.5
60	103541.0
61	103541.0
62	97075.0
63	90609.0
64	76590.5
65	62572.0
66	54599.5
67	46627.0
68	46627.0
69	40203.5
70	33780.0
71	28988.5
72	24197.0
73	19975.5
74	15754.0
75	15754.0
76	12742.5
77	9731.0
78	7995.5
79	6260.0
80	4962.0
81	3664.0
82	3664.0
83	2874.0
84	2084.0
85	1586.0
86	1088.0
87	813.5
88	539.0
89	539.0
90	396.5
91	254.0
92	170.5
93	87.0
94	53.5
95	20.0
96	20.0
97	12.5
98	5.0
99	4.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1322550.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.500638947993494
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.71730496230181	41.08843420623658
2	8.011501955473321	7.771259275471108
3	2.4173086535152106	3.517230426900046
4	1.1628463478566933	2.255951634775613
5	0.6768573195037245	1.641400623627841
6	0.44341152413170315	1.2903445342374742
7	0.34573340235401234	1.1737803643883322
8	0.26256170186715694	1.0187528243063748
9	0.1989570454680837	0.8684589445566347
>10	1.406987247952899	13.593329234657626
>50	0.20144417723753505	6.863546907862602
>100	0.14101539427581888	13.270358772694276
>500	0.011256214449758217	3.7987120934927043
>1k	0.0028140536124395543	1.8484401567928532
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2658	0.20097538845412272	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2166	0.1637745264829307	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1647	0.12453215379380743	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1568	0.11855884465615665	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1461	0.1104684132925031	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1347	0.10184870137234887	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	7.561150807152848E-5	0.0	0.0	7.561150807152848E-5	0.0
12	7.561150807152848E-5	0.0	0.0	7.561150807152848E-5	0.0
13	7.561150807152848E-5	0.0	0.0	7.561150807152848E-5	7.561150807152848E-5
14	7.561150807152848E-5	0.0	0.0	3.0244603228611394E-4	7.561150807152848E-5
15	7.561150807152848E-5	0.0	0.0	4.536690484291709E-4	7.561150807152848E-5
16	7.561150807152848E-5	0.0	0.0	7.561150807152848E-4	7.561150807152848E-5
17	1.5122301614305697E-4	0.0	0.0	0.0011341726210729274	7.561150807152848E-5
18	1.5122301614305697E-4	0.0	0.0	0.0012097841291444558	7.561150807152848E-5
19	1.5122301614305697E-4	0.0	0.0	0.0016634531775736267	7.561150807152848E-5
20	1.5122301614305697E-4	0.0	0.0	0.002192733734074326	7.561150807152848E-5
21	1.5122301614305697E-4	0.0	0.0	0.003931798419719482	7.561150807152848E-5
22	1.5122301614305697E-4	0.0	0.0	0.005670863105364636	7.561150807152848E-5
23	1.5122301614305697E-4	0.0	0.0	0.006956258742580621	7.561150807152848E-5
24	1.5122301614305697E-4	0.0	0.0	0.009375827000869532	7.561150807152848E-5
25	1.5122301614305697E-4	0.0	0.0	0.011871006767229972	7.561150807152848E-5
26	1.5122301614305697E-4	0.0	0.0	0.01595402820309251	7.561150807152848E-5
27	1.5122301614305697E-4	0.0	0.0	0.045669350875203206	7.561150807152848E-5
28	1.5122301614305697E-4	0.0	0.0	0.164530641563646	7.561150807152848E-5
29	1.5122301614305697E-4	0.0	0.0	0.33881516766851916	7.561150807152848E-5
30	1.5122301614305697E-4	0.0	0.0	0.5722278930853276	7.561150807152848E-5
31	1.5122301614305697E-4	0.0	0.0	1.2431288042039998	7.561150807152848E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATAGA	25	0.0054966384	29.6	4
GGTATCA	1500	0.0	29.353333	1
TTTTACG	55	1.9029396E-5	23.545454	4
ATACACA	315	0.0	21.730158	37
CTAGATA	60	3.7268328E-5	21.583334	3
TTACGAG	60	3.7268328E-5	21.583334	6
GTATCAA	2095	0.0	20.9284	2
TATTAGA	205	0.0	18.951221	2
ATACGGC	150	1.2732926E-11	18.5	29
GCGGTAA	1265	0.0	18.280632	23
GGTAATA	1285	0.0	17.996109	25
CTTATAC	1710	0.0	17.959064	37
TAATACG	1190	0.0	17.722689	27
AACTCCG	1290	0.0	17.639534	5
CGTGCCA	1375	0.0	17.490908	10
CGGTAAT	1325	0.0	17.313208	24
TATACAC	580	0.0	17.224138	37
TCTTATA	3120	0.0	17.195513	37
TAGATAA	65	0.0015801042	17.076923	4
TAACTCC	1345	0.0	17.055761	4
>>END_MODULE