FastQCFastQC Report
Fri 10 Feb 2017
ERR1632557.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632557.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483728
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8640.1786127741209936No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA7290.15070452816458837No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6470.13375285284292No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6450.13333939734726954No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5680.11742136076472728No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5540.11452717229517415No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5300.10956570634736877No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5290.10935897859954355No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5250.10853206760824265No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5240.10832533986041741No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5190.1072917011212913No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5060.10460424039956338No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4920.10171005193001025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5150.029.8155351
CCTAGAT250.005493236229.5999983
CCGACTA358.861938E-426.42857214
TCACGTA509.076884E-625.89999825
ATACGGC601.3347326E-624.66666629
CTTATAC5300.024.08490637
GCGGTAA4100.022.10975523
ATCACGT603.720547E-521.58333224
GTATCAA7150.021.2167842
TAATACG4100.021.20731527
CACGACA450.003822653320.5555554
AGTATTA450.003822653320.55555515
GACTCTA450.003822653320.55555525
TGCTATA555.1371765E-420.1818182
AATACGG4400.020.18181828
GTAATAC4700.020.07446926
CACGTAG656.891573E-519.92307726
CGCGGTA4700.019.68085122
CGTGCCA5000.019.60999910
ATACGGA3800.019.47368429
Produced by FastQC (version 0.11.5)