##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632557.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	483728
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.92835643171369	34.0	33.0	34.0	31.0	34.0
2	33.072929414877784	34.0	33.0	34.0	31.0	34.0
3	33.14636324546026	34.0	33.0	34.0	31.0	34.0
4	36.484284556610326	37.0	37.0	37.0	35.0	37.0
5	36.4283460953263	37.0	37.0	37.0	35.0	37.0
6	36.46953246452552	37.0	37.0	37.0	35.0	37.0
7	36.468916415837	37.0	37.0	37.0	35.0	37.0
8	36.44302376542189	37.0	37.0	37.0	35.0	37.0
9	38.20224795752985	39.0	39.0	39.0	37.0	39.0
10	38.21844300929448	39.0	39.0	39.0	37.0	39.0
11	38.29860376079119	39.0	39.0	39.0	37.0	39.0
12	38.26000769027222	39.0	39.0	39.0	37.0	39.0
13	38.29205049118513	39.0	39.0	39.0	37.0	39.0
14	39.80704652201237	41.0	40.0	41.0	38.0	41.0
15	39.79259625243939	41.0	40.0	41.0	38.0	41.0
16	39.75916010650614	41.0	40.0	41.0	38.0	41.0
17	39.75298101412364	41.0	40.0	41.0	38.0	41.0
18	39.75252414580095	41.0	40.0	41.0	38.0	41.0
19	39.75585039526345	41.0	40.0	41.0	38.0	41.0
20	39.73023062547547	41.0	40.0	41.0	38.0	41.0
21	39.69711904210631	41.0	40.0	41.0	38.0	41.0
22	39.66570055899183	41.0	40.0	41.0	37.0	41.0
23	39.630259153904674	41.0	40.0	41.0	37.0	41.0
24	39.61289402308736	41.0	40.0	41.0	37.0	41.0
25	39.56408973638077	41.0	40.0	41.0	37.0	41.0
26	39.475192670260974	41.0	39.0	41.0	37.0	41.0
27	39.405761088876396	41.0	39.0	41.0	37.0	41.0
28	39.35687411107068	41.0	39.0	41.0	36.0	41.0
29	39.31641749082129	41.0	39.0	41.0	36.0	41.0
30	39.2665774980981	41.0	39.0	41.0	36.0	41.0
31	39.20727764363444	40.0	39.0	41.0	36.0	41.0
32	39.15521326034466	40.0	39.0	41.0	36.0	41.0
33	39.093296232593524	40.0	39.0	41.0	35.0	41.0
34	39.0539352694076	40.0	39.0	41.0	35.0	41.0
35	38.97267265901498	40.0	39.0	41.0	35.0	41.0
36	38.92118504614163	40.0	39.0	41.0	35.0	41.0
37	38.867783134323425	40.0	39.0	41.0	35.0	41.0
38	38.796807296662585	40.0	38.0	41.0	35.0	41.0
39	38.71107523236199	40.0	38.0	41.0	35.0	41.0
40	38.63700261303873	40.0	38.0	41.0	35.0	41.0
41	38.58406170409817	40.0	38.0	41.0	35.0	41.0
42	38.5170343664208	40.0	38.0	41.0	35.0	41.0
43	37.76811348526444	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	1.0
16	0.0
17	4.0
18	11.0
19	30.0
20	35.0
21	62.0
22	137.0
23	196.0
24	317.0
25	432.0
26	625.0
27	915.0
28	1244.0
29	1735.0
30	2372.0
31	3183.0
32	4244.0
33	5541.0
34	8564.0
35	13211.0
36	20869.0
37	40635.0
38	112551.0
39	266811.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.17715741077631	16.37366453874905	12.618661727251679	27.83051632322297
2	21.364279098997784	19.03693811398141	32.150919525022324	27.447863261998478
3	21.44242218767572	19.61391525816161	28.49142493302021	30.45223762114246
4	16.16321569146297	15.50664836437006	32.83208745410644	35.498048490060526
5	17.50859987430953	32.62081169582906	32.78722753282837	17.083360897033042
6	35.488745741408394	32.58980253365527	14.99892501571131	16.922526709225018
7	30.844813614262563	27.39059967585089	19.555411305527073	22.209175404359474
8	27.5340687328416	30.88636589157543	19.923179968908148	21.656385406674826
9	27.991350511030994	13.210109813779644	17.49247510997916	41.3060645652102
10	19.34702973571925	23.56034796414514	29.965187047266234	27.127435252869383
11	39.09614494095855	19.533704892005424	18.68508748718288	22.68506267985314
12	23.15474812291205	23.542982833327823	26.429315648463596	26.872953395296527
13	34.72013197499421	17.516042073231237	21.09491284358152	26.66891310819303
14	24.6754374359144	19.606886514735553	22.677000297687957	33.04067575166209
15	29.343970165051438	23.954577779247842	19.955222769821056	26.746229285879668
16	26.16387722025601	23.725109979161843	22.12503721099461	27.985975589587536
17	26.754498395792677	23.653168722918664	21.949111897595344	27.64322098369332
18	26.613510071775874	21.658659411900903	23.48530579168458	28.242524724638642
19	28.27849535276023	22.38075943505441	22.962698045182417	26.378047167002943
20	29.56620249396355	21.178637581450733	22.61188105712301	26.643278867462705
21	27.606423444580425	22.178372969933516	21.968130850395262	28.247072735090796
22	28.135439751265174	22.801450401878743	21.31735190024146	27.745757946614624
23	28.231774881751726	22.24142493302021	22.24535276022889	27.281447424999172
24	28.03993153176992	21.786830615552542	22.340654251976318	27.83258360070122
25	28.404806006681444	22.06260543115139	22.61580888433169	26.91677967783548
26	28.071354149439355	22.728888962392087	22.202353388681242	26.997403499487316
27	27.229972215790692	22.03097608573413	23.442719875632587	27.29633182284259
28	26.51448748056759	22.84693050640029	22.342308073958918	28.296273939073195
29	27.22645784407766	23.248395792676877	22.323495848906823	27.20165051433864
30	27.06975821122614	22.616429067575165	23.394759038137135	26.919053683061556
31	27.777594019779713	22.238944200046305	22.455388152019317	27.528073628154665
32	25.6941917771971	22.35429828333278	22.85478616081765	29.096723778652468
33	26.28212549201204	21.910867264247678	24.05628783117785	27.75071941256243
34	26.398099758541992	21.554675354744816	23.98579366916945	28.061431217543742
35	26.270962193629476	22.718966030496475	24.421575761585025	26.58849601428902
36	26.260212350742567	21.488522475440742	24.962582277643637	27.288682896173057
37	26.527511328680582	21.053774021764298	24.781695498296564	27.637019151258556
38	25.587933714814938	20.489820725697086	26.120050937717064	27.802194621770916
39	25.143262329242884	19.75200939370886	27.127228525121556	27.977499751926704
40	24.498478483776008	20.576646379783682	28.09698839016968	26.82788674627063
41	23.229583567624783	19.868810571230114	28.971446763470382	27.93015909767473
42	21.96523666192571	20.685178447391923	29.62036516389376	27.729219726788607
43	21.301433863658918	20.94834287037343	29.429968908146726	28.32025435782092
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	6.5
2	5.0
3	9.5
4	14.0
5	14.0
6	22.5
7	31.0
8	31.0
9	31.0
10	46.5
11	62.0
12	62.0
13	123.5
14	185.0
15	330.5
16	476.0
17	479.5
18	483.0
19	483.0
20	613.5
21	744.0
22	730.0
23	716.0
24	886.5
25	1057.0
26	1057.0
27	1298.0
28	1539.0
29	2130.5
30	2722.0
31	3379.5
32	4037.0
33	4037.0
34	5163.0
35	6289.0
36	7125.5
37	7962.0
38	9992.5
39	12023.0
40	12023.0
41	14097.5
42	16172.0
43	18855.0
44	21538.0
45	27312.0
46	33086.0
47	33086.0
48	36998.0
49	40910.0
50	45258.0
51	49606.0
52	51745.5
53	53885.0
54	53885.0
55	50970.0
56	48055.0
57	46036.0
58	44017.0
59	40759.0
60	37501.0
61	37501.0
62	34363.5
63	31226.0
64	26470.5
65	21715.0
66	18908.5
67	16102.0
68	16102.0
69	13761.5
70	11421.0
71	9798.5
72	8176.0
73	6544.5
74	4913.0
75	4913.0
76	3889.5
77	2866.0
78	2380.5
79	1895.0
80	1483.5
81	1072.0
82	1072.0
83	834.5
84	597.0
85	454.5
86	312.0
87	238.5
88	165.0
89	165.0
90	118.0
91	71.0
92	50.0
93	29.0
94	20.5
95	12.0
96	12.0
97	7.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	483728.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.77631735147332
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.94498973672499	49.364163294128886
2	6.747382078934838	7.661830124104963
3	1.9793464400377807	3.3714000488428195
4	0.9697175324783271	2.2022796146110855
5	0.5902758760661498	1.6756845232225326
6	0.4207828539860679	1.433430051238299
7	0.31752271548193695	1.2619439322352815
8	0.2411072891558634	1.0951347171893393
9	0.19578651994522095	1.0004433830595383
>10	1.3693330811522675	15.235252245465036
>50	0.13315308690432326	5.180955236667204
>100	0.0862010341946453	9.03048598955386
>500	0.004401754937598909	1.486996839681155
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	864	0.1786127741209936	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	729	0.15070452816458837	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	647	0.13375285284292	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	645	0.13333939734726954	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	568	0.11742136076472728	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	554	0.11452717229517415	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	530	0.10956570634736877	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	529	0.10935897859954355	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	525	0.10853206760824265	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	524	0.10832533986041741	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	519	0.1072917011212913	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	506	0.10460424039956338	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	492	0.10171005193001025	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	2.0672774782522408E-4	0.0	0.0	0.0
11	0.0	2.0672774782522408E-4	0.0	0.0	0.0
12	0.0	2.0672774782522408E-4	0.0	0.0	0.0
13	0.0	2.0672774782522408E-4	0.0	0.0	0.0
14	0.0	2.0672774782522408E-4	0.0	0.0	0.0
15	0.0	2.0672774782522408E-4	0.0	0.0	0.0
16	2.0672774782522408E-4	2.0672774782522408E-4	0.0	0.0	0.0
17	2.0672774782522408E-4	2.0672774782522408E-4	0.0	2.0672774782522408E-4	0.0
18	2.0672774782522408E-4	2.0672774782522408E-4	0.0	2.0672774782522408E-4	0.0
19	2.0672774782522408E-4	2.0672774782522408E-4	0.0	6.201832434756723E-4	0.0
20	2.0672774782522408E-4	2.0672774782522408E-4	0.0	0.0010336387391261205	0.0
21	2.0672774782522408E-4	2.0672774782522408E-4	0.0	0.0014470942347765687	0.0
22	2.0672774782522408E-4	2.0672774782522408E-4	0.0	0.0037210994608540336	0.0
23	2.0672774782522408E-4	2.0672774782522408E-4	0.0	0.0047547381999801545	0.0
24	2.0672774782522408E-4	2.0672774782522408E-4	0.0	0.006615287930407171	0.0
25	2.0672774782522408E-4	2.0672774782522408E-4	0.0	0.008889293156484636	0.0
26	2.0672774782522408E-4	2.0672774782522408E-4	0.0	0.011990209373862998	0.0
27	2.0672774782522408E-4	2.0672774782522408E-4	0.0	0.03659081136506467	0.0
28	2.0672774782522408E-4	2.0672774782522408E-4	0.0	0.135613402573347	0.0
29	2.0672774782522408E-4	2.0672774782522408E-4	0.0	0.2910726689379155	0.0
30	2.0672774782522408E-4	2.0672774782522408E-4	0.0	0.5017282439718189	0.0
31	2.0672774782522408E-4	2.0672774782522408E-4	0.0	1.1618099427777595	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	515	0.0	29.815535	1
CCTAGAT	25	0.0054932362	29.599998	3
CCGACTA	35	8.861938E-4	26.428572	14
TCACGTA	50	9.076884E-6	25.899998	25
ATACGGC	60	1.3347326E-6	24.666666	29
CTTATAC	530	0.0	24.084906	37
GCGGTAA	410	0.0	22.109755	23
ATCACGT	60	3.720547E-5	21.583332	24
GTATCAA	715	0.0	21.216784	2
TAATACG	410	0.0	21.207315	27
CACGACA	45	0.0038226533	20.555555	4
AGTATTA	45	0.0038226533	20.555555	15
GACTCTA	45	0.0038226533	20.555555	25
TGCTATA	55	5.1371765E-4	20.181818	2
AATACGG	440	0.0	20.181818	28
GTAATAC	470	0.0	20.074469	26
CACGTAG	65	6.891573E-5	19.923077	26
CGCGGTA	470	0.0	19.680851	22
CGTGCCA	500	0.0	19.609999	10
ATACGGA	380	0.0	19.473684	29
>>END_MODULE