FastQCFastQC Report
Fri 10 Feb 2017
ERR1632556.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632556.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences607765
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA10870.17885202339720122No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA9350.1538423568319992No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8120.13360427138778969No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7980.13130074946731055No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA7930.13047806306713944No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC7540.1240611091458047No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA7270.11961860258488068No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG7190.11830230434460688No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7100.11682146882429888No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA6930.11402433506371705No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT6680.10991090306286147No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6620.10892367938265612No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC6540.10760738114238234No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6380.10497478466183477No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT6230.10250672546132139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6050.028.132231
TACCCGA358.864566E-426.42857211
CTAAGTC851.9717845E-923.94117734
TAGACCC400.001930146223.1254
AACTTAC400.001930146223.1257
TAAGTCT855.1706593E-821.76470635
ATACACA2100.020.26190437
ACTACAC555.139262E-420.1818183
CGCGGTA4100.019.40244122
TCTATGG1355.638867E-1119.1851862
GCGGTAA4050.019.18518423
TTCTATC500.007031159518.518
GAGTAGA500.007031159518.51
TTGTACT500.007031159518.54
TATTAGC701.21799785E-418.52
TATTAGA609.229988E-418.52
TTTTACG500.007031159518.54
GTATCAA9150.018.3989072
CTTATAC8250.018.16363737
CCGCGGT4500.017.67777821
Produced by FastQC (version 0.11.5)