##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632556.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	607765
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.05410643916645	34.0	33.0	34.0	31.0	34.0
2	33.19093399587011	34.0	33.0	34.0	31.0	34.0
3	33.2702162842546	34.0	34.0	34.0	31.0	34.0
4	36.54777257657154	37.0	37.0	37.0	35.0	37.0
5	36.51386967002048	37.0	37.0	37.0	35.0	37.0
6	36.557006408727055	37.0	37.0	37.0	35.0	37.0
7	36.55319901606707	37.0	37.0	37.0	35.0	37.0
8	36.52751145590812	37.0	37.0	37.0	35.0	37.0
9	38.32415654076822	39.0	39.0	39.0	37.0	39.0
10	38.35450708744334	39.0	39.0	39.0	37.0	39.0
11	38.42371804891693	39.0	39.0	39.0	37.0	39.0
12	38.38483295352645	39.0	39.0	39.0	37.0	39.0
13	38.419073161501565	39.0	39.0	39.0	37.0	39.0
14	39.974732009905146	41.0	40.0	41.0	38.0	41.0
15	39.965976981234526	41.0	40.0	41.0	38.0	41.0
16	39.93534672118335	41.0	40.0	41.0	38.0	41.0
17	39.926823690077576	41.0	40.0	41.0	38.0	41.0
18	39.923623439980915	41.0	40.0	41.0	38.0	41.0
19	39.925956578611796	41.0	40.0	41.0	38.0	41.0
20	39.90147343134271	41.0	40.0	41.0	38.0	41.0
21	39.869610787064076	41.0	40.0	41.0	38.0	41.0
22	39.83984105698749	41.0	40.0	41.0	38.0	41.0
23	39.80088027444818	41.0	40.0	41.0	38.0	41.0
24	39.77513512624123	41.0	40.0	41.0	38.0	41.0
25	39.730876243284825	41.0	40.0	41.0	38.0	41.0
26	39.64329140374981	41.0	40.0	41.0	37.0	41.0
27	39.570942716345954	41.0	40.0	41.0	37.0	41.0
28	39.52712314792724	41.0	40.0	41.0	37.0	41.0
29	39.47625151168626	41.0	40.0	41.0	37.0	41.0
30	39.436857996100464	41.0	39.0	41.0	37.0	41.0
31	39.37543293871809	41.0	39.0	41.0	36.0	41.0
32	39.31823648943259	41.0	39.0	41.0	36.0	41.0
33	39.251640025338745	41.0	39.0	41.0	36.0	41.0
34	39.20931610079554	41.0	39.0	41.0	35.0	41.0
35	39.1355951724762	41.0	39.0	41.0	35.0	41.0
36	39.083157141329295	41.0	39.0	41.0	35.0	41.0
37	39.02002254160737	41.0	39.0	41.0	35.0	41.0
38	38.951886008572394	40.0	39.0	41.0	35.0	41.0
39	38.86833233239821	40.0	39.0	41.0	35.0	41.0
40	38.7889480309001	40.0	38.0	41.0	35.0	41.0
41	38.735945636882676	40.0	38.0	41.0	35.0	41.0
42	38.66411359653814	40.0	38.0	41.0	35.0	41.0
43	37.92683356231438	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	1.0
14	0.0
15	0.0
16	1.0
17	3.0
18	5.0
19	15.0
20	27.0
21	57.0
22	111.0
23	201.0
24	332.0
25	510.0
26	714.0
27	1044.0
28	1411.0
29	1921.0
30	2585.0
31	3431.0
32	4611.0
33	6252.0
34	9281.0
35	15061.0
36	23815.0
37	45379.0
38	118464.0
39	372530.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.43932276455538	15.835725979613832	12.282707954554803	28.442243301275987
2	21.50650333599335	18.586953839066087	31.60201722705322	28.30452559788734
3	21.858284040706526	18.962592449384218	27.80367411746316	31.375449392446093
4	16.47100441782597	15.089220340098558	32.191389764135806	36.248385477939664
5	18.222010151950176	31.871858366309347	32.128207448602666	17.777924033137808
6	36.46754913494525	31.78860250261203	14.816088455241747	16.927759907200972
7	31.777578504849735	26.344063906279562	19.302855544494992	22.575502044375703
8	28.29975401676635	30.111309469943155	19.449293723725454	22.139642789565045
9	28.2601005322781	12.749664755291931	17.22985035334381	41.76038435908616
10	19.894367066218027	22.909841797405246	29.357235115546302	27.838556020830417
11	40.35424876391369	18.84346745863944	18.253765846996785	22.54851793045009
12	23.570788051302724	22.600018099100804	26.29355096130906	27.535642888287416
13	35.51849810370785	16.996865564815348	20.164866354594295	27.31976997688251
14	24.81715794756197	18.872755094485534	22.328037975204232	33.98204898274827
15	30.004689312480977	23.393087788865763	19.40980477651724	27.192418122136026
16	26.84178917838309	22.99457849662287	21.788355696691976	28.37527662830206
17	27.491711434518272	22.8086513701842	21.515223811835167	28.18441338346236
18	27.517872862043717	21.096476434148066	22.624863228385973	28.760787475422244
19	28.67736707444489	21.85713227974628	22.309116188000296	27.15638445780853
20	29.921268911503624	20.72330588303045	22.052438031146906	27.30298717431902
21	28.276225185721454	21.608680986894605	21.568204816006187	28.54688901137775
22	28.996075785871184	22.05984220874845	20.86595970482012	28.07812230056025
23	29.00759339547358	21.481000057588048	21.583342245769334	27.928064301169037
24	28.526650925933545	21.104209686309673	21.824553898299506	28.544585489457276
25	28.884354972727944	21.234358674816747	22.139642789565045	27.74164356289026
26	28.562190978420936	21.942362590804013	21.89678576423453	27.59866066654052
27	27.76286887201468	21.40893272893306	23.182315533141924	27.645882865910345
28	27.298544667758094	22.207761223499215	21.634677877140014	28.859016231602673
29	28.088981761042508	22.718814015285513	21.6504734560233	27.54173076764868
30	27.739010966409715	21.547802193281942	22.73378690776863	27.979399932539717
31	28.39485656462613	21.482645430388388	21.992381923934413	28.130116081051064
32	26.711804727156057	21.610326359694948	22.169917649091342	29.507951264057652
33	26.883252572951715	21.152748183919773	23.6471333492386	28.316865893889908
34	27.04005660082433	20.850328663216867	23.46038353639976	28.64923119955904
35	26.58066851496878	22.07711862315204	24.003192023232664	27.339020838646515
36	26.75984961292605	20.88504602930409	24.469655212129688	27.885449145640173
37	26.782062145730666	20.44721232713302	24.625307479042064	28.145418048094246
38	25.88697934234449	19.818679917402285	25.936340526354755	28.358000213898464
39	25.353549480473536	18.974932745386784	27.153587324047944	28.51793045009173
40	24.962773440392258	19.97301588607439	27.841024080030934	27.223186593502426
41	23.545778384737524	19.393351048513818	28.75173792502036	28.3091326417283
42	22.131909537403438	20.110569052182996	29.632999596883664	28.124521813529903
43	21.526905958717595	20.423683496088127	29.28763584609183	28.76177469910245
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	3.0
2	5.0
3	10.5
4	16.0
5	16.0
6	22.5
7	29.0
8	26.5
9	24.0
10	40.5
11	57.0
12	57.0
13	128.5
14	200.0
15	327.0
16	454.0
17	446.5
18	439.0
19	439.0
20	548.5
21	658.0
22	574.5
23	491.0
24	621.0
25	751.0
26	751.0
27	975.0
28	1199.0
29	1565.5
30	1932.0
31	2480.5
32	3029.0
33	3029.0
34	4263.5
35	5498.0
36	6410.0
37	7322.0
38	9546.5
39	11771.0
40	11771.0
41	14396.5
42	17022.0
43	20433.0
44	23844.0
45	31981.0
46	40118.0
47	40118.0
48	45505.5
49	50893.0
50	56723.5
51	62554.0
52	63882.5
53	65211.0
54	65211.0
55	63062.5
56	60914.0
57	58900.0
58	56886.0
59	53914.5
60	50943.0
61	50943.0
62	47452.0
63	43961.0
64	37634.5
65	31308.0
66	27565.5
67	23823.0
68	23823.0
69	20420.0
70	17017.0
71	14370.0
72	11723.0
73	9449.0
74	7175.0
75	7175.0
76	5801.5
77	4428.0
78	3620.5
79	2813.0
80	2177.0
81	1541.0
82	1541.0
83	1212.0
84	883.0
85	685.0
86	487.0
87	351.5
88	216.0
89	216.0
90	151.0
91	86.0
92	60.0
93	34.0
94	20.0
95	6.0
96	6.0
97	4.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	607765.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.83374121154411
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.42883608626583	52.31194352812138
2	6.951594440060793	8.318798054684127
3	1.9545612972343414	3.5084614452245857
4	0.9735887453113646	2.3301382813372844
5	0.5413115932534617	1.6194348892768127
6	0.36324438441140816	1.3040562296051306
7	0.2421821532302261	1.014346499770231
8	0.19329459099845778	0.9252430828314389
9	0.15203017384183448	0.8186880670198549
>10	1.0076482578311838	11.79265849804452
>50	0.10950818753600365	4.674733050994855
>100	0.07668330545962512	9.070869353031027
>500	0.005240945337168282	2.131225181144206
>1k	2.758392282720149E-4	0.179403838914563
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1087	0.17885202339720122	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	935	0.1538423568319992	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	812	0.13360427138778969	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	798	0.13130074946731055	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	793	0.13047806306713944	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	754	0.1240611091458047	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	727	0.11961860258488068	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	719	0.11830230434460688	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	710	0.11682146882429888	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	693	0.11402433506371705	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	668	0.10991090306286147	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	662	0.10892367938265612	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	654	0.10760738114238234	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	638	0.10497478466183477	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	623	0.10250672546132139	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.6453728003422376E-4	0.0
10	1.6453728003422376E-4	0.0	0.0	1.6453728003422376E-4	0.0
11	1.6453728003422376E-4	1.6453728003422376E-4	0.0	1.6453728003422376E-4	0.0
12	1.6453728003422376E-4	1.6453728003422376E-4	0.0	1.6453728003422376E-4	0.0
13	1.6453728003422376E-4	1.6453728003422376E-4	0.0	1.6453728003422376E-4	0.0
14	1.6453728003422376E-4	1.6453728003422376E-4	0.0	1.6453728003422376E-4	0.0
15	4.936118401026713E-4	1.6453728003422376E-4	0.0	1.6453728003422376E-4	0.0
16	4.936118401026713E-4	1.6453728003422376E-4	0.0	3.290745600684475E-4	0.0
17	4.936118401026713E-4	1.6453728003422376E-4	0.0	6.58149120136895E-4	0.0
18	4.936118401026713E-4	1.6453728003422376E-4	0.0	8.226864001711188E-4	0.0
19	6.58149120136895E-4	1.6453728003422376E-4	0.0	9.872236802053425E-4	0.0
20	6.58149120136895E-4	1.6453728003422376E-4	0.0	0.0016453728003422376	0.0
21	6.58149120136895E-4	1.6453728003422376E-4	0.0	0.00263259648054758	0.0
22	6.58149120136895E-4	1.6453728003422376E-4	0.0	0.00394889472082137	0.0
23	6.58149120136895E-4	1.6453728003422376E-4	0.0	0.004771581120992489	0.0
24	6.58149120136895E-4	1.6453728003422376E-4	0.0	0.006746028481403174	0.0
25	6.58149120136895E-4	1.6453728003422376E-4	0.0	0.009378624961950754	0.0
26	6.58149120136895E-4	1.6453728003422376E-4	0.0	0.01497289248311436	0.0
27	6.58149120136895E-4	1.6453728003422376E-4	0.0	0.04886757217016446	0.0
28	6.58149120136895E-4	1.6453728003422376E-4	0.0	0.16914432387518202	0.0
29	6.58149120136895E-4	1.6453728003422376E-4	0.0	0.34783180999234903	0.0
30	6.58149120136895E-4	1.6453728003422376E-4	0.0	0.5906888353228633	0.0
31	6.58149120136895E-4	1.6453728003422376E-4	0.0	1.2774674421857133	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	605	0.0	28.13223	1
TACCCGA	35	8.864566E-4	26.428572	11
CTAAGTC	85	1.9717845E-9	23.941177	34
TAGACCC	40	0.0019301462	23.125	4
AACTTAC	40	0.0019301462	23.125	7
TAAGTCT	85	5.1706593E-8	21.764706	35
ATACACA	210	0.0	20.261904	37
ACTACAC	55	5.139262E-4	20.181818	3
CGCGGTA	410	0.0	19.402441	22
TCTATGG	135	5.638867E-11	19.185186	2
GCGGTAA	405	0.0	19.185184	23
TTCTATC	50	0.0070311595	18.5	18
GAGTAGA	50	0.0070311595	18.5	1
TTGTACT	50	0.0070311595	18.5	4
TATTAGC	70	1.21799785E-4	18.5	2
TATTAGA	60	9.229988E-4	18.5	2
TTTTACG	50	0.0070311595	18.5	4
GTATCAA	915	0.0	18.398907	2
CTTATAC	825	0.0	18.163637	37
CCGCGGT	450	0.0	17.677778	21
>>END_MODULE