FastQCFastQC Report
Fri 10 Feb 2017
ERR1632555.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632555.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629800
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA32670.5187361067005398No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA27210.4320419180692283No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA23050.3659892029215624No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA19080.3029533185138139No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT16510.26214671324229916No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA16480.26167037154652273No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA15730.24976182915211176No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA14220.2257859637980311No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT13570.2154652270562083No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC13220.20990790727214986No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC13060.2073674182280089No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC13030.20689107653223243No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG12710.20181009844395045No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT12410.1970466814861861No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC12370.19641155922515086No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC12360.19625277865989205No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA12160.19307716735471578No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT11820.18767862813591618No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT11780.18704350587488092No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC11540.1832327723086694No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA11340.18005716100349317No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA11310.17958081930771674No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC11200.17783423308986981No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA11010.17481740234995236No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC10960.1740234995236583No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG10830.17195935217529373No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC10820.17180057161003492No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG10580.16798983804382342No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC10500.16671959352175292No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA10480.1664020323912353No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC10240.16259129882502382No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG10060.1597332486503652No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA9400.1492537313432836No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9380.14893617021276595No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC9350.14845982851698952No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA9100.1444903143855192No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC9030.14337885042870752No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC8780.1394093362972372No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC8700.1381390917751667No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT8630.13702762781835504No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA8440.1340107970784376No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA8350.1325817719911083No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8200.1302000635122261No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT8130.1290885995554144No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT8120.1289298189901556No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG8050.12781835503334393No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG8000.12702445220704986No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT7910.12559542711972055No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA7880.1251190854239441No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC7860.12480152429342649No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG7840.12448396316290886No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG7800.1238488409018736No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC7750.12305493807557955No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC7750.12305493807557955No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT7680.12194347411876787No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC7570.12019688790092092No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA7570.12019688790092092No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC7550.1198793267704033No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG7400.11749761829152111No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC7370.11702127659574468No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC7350.11670371546522705No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA7260.11527469037789775No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG7200.11432200698634487No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC7030.11162273737694506No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT7020.11146395681168625No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT6870.10908224833280405No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC6860.10892346776754526No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG6850.10876468720228644No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG6810.1081295649412512No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA6740.1070181009844395No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT6720.10670053985392187No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC6690.10622419815814543No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT6680.10606541759288664No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG6530.10368370911400444No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG6520.10352492854874563No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA6480.10288980628771038No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG6410.1017783423308987No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC6360.10098443950460465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCT502.7174792E-729.59999833
GGTATCA11350.029.5022031
ATACGGC1400.029.0714329
TACGGCT1550.026.25806430
AGAGACA451.3222845E-424.66666616
CTTTTTA551.900889E-523.5454542
ATTACCT652.6796479E-622.7692333
TTTTTAC652.6796479E-622.769233
CACAAAC502.7002237E-422.19999937
GCGGTAA12000.021.737523
TATACAC3000.021.58333437
TTTACGA603.722845E-521.5833325
TTCCCCT603.722845E-521.58333232
CGCGGTA12250.021.29387722
TCAATGC806.949831E-720.812532
TTAATGT450.003823916720.55555535
AAGAGAC450.003823916720.55555515
TGTATAA450.003823916720.55555510
TAAGAGA450.003823916720.55555514
AATGTTT450.003823916720.55555537
Produced by FastQC (version 0.11.5)