FastQCFastQC Report
Fri 10 Feb 2017
ERR1632554.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632554.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26994
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT850.3148847892124176TruSeq Adapter, Index 3 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA800.29636215455286363No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA800.29636215455286363No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA660.24449877750611246No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC590.2185670889827369No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA590.2185670889827369No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA560.20745350818700453No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG550.20374898125509372No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC540.20004445432318294No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT540.20004445432318294No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT540.20004445432318294No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA510.18893087352745055No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA470.17411276579980736No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA470.17411276579980736No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT380.14077202341261022No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA370.1370674964806994No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT360.13336296954878862No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG350.1296584426168778No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA340.12595391568496703No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT340.12595391568496703No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA330.12224938875305623No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA320.11854486182114544No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC320.11854486182114544No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC310.11484033488923465No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC300.11113580795732383No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT290.10743128102541305No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA290.10743128102541305No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT290.10743128102541305No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG290.10743128102541305No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA290.10743128102541305No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC270.10002222716159147No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC270.10002222716159147RNA PCR Primer, Index 3 (95% over 22bp)
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC270.10002222716159147No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG270.10002222716159147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA551.7178536E-830.272731
GGTAATA250.00540280729.59999825
GTATCAA701.782355E-723.7857152
AACGCAG901.9982708E-618.57
ACGCAGA901.9982708E-618.58
CGCAGAG901.9982708E-618.59
CAACGCA901.9982708E-618.56
TCAACGC953.3498363E-617.5263165
TATCAAC953.3498363E-617.5263163
ATCAACG953.3498363E-617.5263164
CAGAGTA1005.461956E-616.6511
AGAGTAC1005.461956E-616.6512
GCAGAGT1058.685669E-615.85714410
GTCTCTT1501.1755528E-613.56666737
GAGTACG1153.379689E-412.86956513
AGTACGG1204.840501E-412.33333414
CTGTCTC2603.2177923E-911.38461537
TGTCTCT1901.6510106E-510.71052636
GTACGGG1500.00312684859.86666715
CCTGTCT1707.8681845E-49.79411837
Produced by FastQC (version 0.11.5)