FastQCFastQC Report
Fri 10 Feb 2017
ERR1632552.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632552.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45425
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1370.30159603742432584No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1060.2333516785910842No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA860.18932305998899285No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT750.1651073197578426No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA660.14529444138690148No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA650.14309301045679693No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA640.14089157952669235No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA630.1386901485965878No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA630.1386901485965878No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT610.13428728673637863No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA580.12768299394606494No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT580.12768299394606494No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC580.12768299394606494No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG580.12768299394606494No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA570.1254815630159604No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC550.12107870115575124No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT540.11887727022564668No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT520.11447440836543754No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC490.10787011557512384No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG490.10787011557512384No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC490.10787011557512384No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC490.10787011557512384No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC470.10346725371491469No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA470.10346725371491469No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA460.10126582278481014No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTCGC200.001823379437.023
CGGTTCG200.001823379437.022
GGTATCA603.274181E-1133.9166681
TTTCAGT250.00544160229.610
TCAGTTC250.00544160229.612
GAGTACT250.00544160229.613
AAGCTGC250.00544160229.66
GTTCGCC250.00544160229.624
GTATCAA701.9463187E-1029.071432
ACTTAGA358.738317E-426.4285721
CCCCCGG358.738317E-426.42857218
AACGCAG809.1677066E-1025.4375027
ACGCAGA809.1677066E-1025.4375028
TCAACGC809.1677066E-1025.4375025
ATCAACG809.1677066E-1025.4375024
CAACGCA851.8517312E-923.9411756
CGCAGAG903.588866E-922.6111139
TATCAAC903.588866E-922.6111133
AGAGTAC1004.947651E-1022.212
GCAGAGT956.701157E-921.42105310
Produced by FastQC (version 0.11.5)