##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632552.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	45425
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.00290588882774	34.0	33.0	34.0	31.0	34.0
2	33.14637314254265	34.0	33.0	34.0	31.0	34.0
3	33.21430930104568	34.0	33.0	34.0	31.0	34.0
4	36.50307099614749	37.0	37.0	37.0	35.0	37.0
5	36.480990643918545	37.0	37.0	37.0	35.0	37.0
6	36.52504127682994	37.0	37.0	37.0	35.0	37.0
7	36.53362685745735	37.0	37.0	37.0	35.0	37.0
8	36.49723720418272	37.0	37.0	37.0	35.0	37.0
9	38.288277380297195	39.0	39.0	39.0	37.0	39.0
10	38.32867363786461	39.0	39.0	39.0	37.0	39.0
11	38.36733076499725	39.0	39.0	39.0	37.0	39.0
12	38.34868464501926	39.0	39.0	39.0	37.0	39.0
13	38.38901485965878	39.0	39.0	39.0	37.0	39.0
14	39.91916345624656	41.0	40.0	41.0	38.0	41.0
15	39.90084755090809	41.0	40.0	41.0	38.0	41.0
16	39.857347275729225	41.0	40.0	41.0	38.0	41.0
17	39.8758172812328	41.0	40.0	41.0	38.0	41.0
18	39.87187671986791	41.0	40.0	41.0	38.0	41.0
19	39.88693450742983	41.0	40.0	41.0	38.0	41.0
20	39.870886075949365	41.0	40.0	41.0	38.0	41.0
21	39.822344523940565	41.0	40.0	41.0	38.0	41.0
22	39.78916895982388	41.0	40.0	41.0	38.0	41.0
23	39.75616951018162	41.0	40.0	41.0	38.0	41.0
24	39.74835443037975	41.0	40.0	41.0	38.0	41.0
25	39.69946064942212	41.0	40.0	41.0	38.0	41.0
26	39.6020913593836	41.0	40.0	41.0	37.0	41.0
27	39.526582278481015	41.0	40.0	41.0	37.0	41.0
28	39.46901485965878	41.0	40.0	41.0	37.0	41.0
29	39.423643368189325	41.0	39.0	41.0	37.0	41.0
30	39.38837644468905	41.0	39.0	41.0	36.0	41.0
31	39.34571271326362	41.0	39.0	41.0	36.0	41.0
32	39.2799559713814	41.0	39.0	41.0	36.0	41.0
33	39.218227848101264	41.0	39.0	41.0	35.0	41.0
34	39.19031370390754	41.0	39.0	41.0	35.0	41.0
35	39.097985690698955	41.0	39.0	41.0	35.0	41.0
36	39.05725921849202	41.0	39.0	41.0	35.0	41.0
37	39.01765547605944	41.0	39.0	41.0	35.0	41.0
38	38.90298293891029	40.0	39.0	41.0	35.0	41.0
39	38.8062740781508	40.0	38.0	41.0	35.0	41.0
40	38.71680792515135	40.0	38.0	41.0	35.0	41.0
41	38.66846450192625	40.0	38.0	41.0	35.0	41.0
42	38.584700055035775	40.0	38.0	41.0	35.0	41.0
43	37.84305998899285	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	0.0
20	2.0
21	7.0
22	10.0
23	20.0
24	35.0
25	39.0
26	62.0
27	90.0
28	118.0
29	172.0
30	197.0
31	267.0
32	394.0
33	492.0
34	765.0
35	1130.0
36	1826.0
37	3400.0
38	8827.0
39	27571.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.960374243258116	16.114474408365435	12.328013208585581	27.597138139790868
2	22.141992294991745	18.932305998899285	31.399009356081454	27.526692350027517
3	22.24105668684645	19.19427627958173	28.383048981838193	30.18161805173363
4	16.233351678591085	15.771051183269124	32.973032471106215	35.02256466703357
5	18.445789763346173	31.896532746285082	31.80187121629059	17.85580627407815
6	34.48541552008806	32.992845349477165	15.009356081452943	17.51238304898184
7	30.221243808475506	26.969730324711062	19.531095211887727	23.2779306549257
8	27.808475509080903	29.979086406164008	19.65877820583379	22.553659878921298
9	28.072647220693455	12.803522289488168	17.067694001100715	42.05613648871767
10	20.171711612548158	22.326912493120528	28.8189323059989	28.68244358833242
11	40.03742432581178	18.87286736378646	18.373142542652722	22.716565767749035
12	22.837644468904788	23.070996147495872	26.263070996147498	27.828288387451845
13	35.539900935608145	17.34507429829389	19.77765547605944	27.337369290038527
14	24.618602091359385	19.33957072096863	22.63511282333517	33.40671436433682
15	30.632911392405067	23.343973582828838	19.20528343423225	26.817831590533846
16	25.970280682443587	23.25811777655476	22.40396257567419	28.367638965327462
17	27.482663731425426	22.985140341221793	21.19977985690699	28.332416070445788
18	27.14144193725922	21.03247110621904	22.91029168959824	28.9157952669235
19	28.40066042927903	22.309301045679693	22.696752889378097	26.593285635663182
20	30.300495321959275	20.52394056136489	21.61805173362686	27.557512383048984
21	28.03302146395157	21.761144744083655	21.450742982938912	28.75509080902587
22	28.488717666483215	22.68354430379747	21.003852504127682	27.823885525591635
23	28.645019262520638	21.505778756191525	21.831590533847	28.01761144744084
24	28.71766648321409	21.367088607594937	21.888827738029722	28.026417171161256
25	29.023665382498624	21.743533296642816	21.946064942212438	27.286736378646122
26	28.444689047881123	22.359933957072098	22.06714364336819	27.128233351678592
27	27.988992845349475	21.309851403412218	23.082003302146394	27.61915244909191
28	27.38800220143093	22.736378646119977	21.446340121078702	28.429279031370392
29	28.15410016510732	22.98293891029169	21.574023115024765	27.288937809576225
30	27.16565767749037	21.587231700605393	23.568519537699505	27.678591084204733
31	28.385250412768297	21.688497523390204	21.963676389653276	27.962575674188223
32	26.210236653824985	21.560814529444137	22.709961474958725	29.518987341772153
33	26.588882773802975	21.496973032471107	24.332416070445788	27.58172812328013
34	26.969730324711062	21.025866813428728	23.66758392955421	28.336818932306002
35	25.987892129884425	22.787011557512383	23.982388552559165	27.242707760044027
36	26.54925701706109	20.895982388552557	24.99284534947716	27.56191524490919
37	26.982938910291686	20.184920198128783	24.59218492019813	28.2399559713814
38	25.81838194826637	20.006604292790314	25.824986241056685	28.350027517886627
39	25.294441386901482	18.76719867914144	27.47826086956522	28.46009906439185
40	24.84314804623005	20.246560264171713	27.81067694001101	27.099614749587232
41	23.337369290038527	19.71601541001651	28.799119427627957	28.14749587231701
42	21.939460649422124	20.508530544854157	29.574023115024765	27.977985690698954
43	21.38690148596588	21.07429829389103	29.105118326912493	28.4336818932306
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	2.5
4	5.0
5	5.0
6	4.0
7	3.0
8	4.0
9	5.0
10	9.0
11	13.0
12	13.0
13	22.0
14	31.0
15	41.0
16	51.0
17	52.5
18	54.0
19	54.0
20	63.5
21	73.0
22	60.0
23	47.0
24	44.0
25	41.0
26	41.0
27	62.0
28	83.0
29	105.0
30	127.0
31	167.5
32	208.0
33	208.0
34	272.5
35	337.0
36	384.5
37	432.0
38	652.0
39	872.0
40	872.0
41	1060.5
42	1249.0
43	1504.0
44	1759.0
45	2418.0
46	3077.0
47	3077.0
48	3544.0
49	4011.0
50	4412.0
51	4813.0
52	5046.0
53	5279.0
54	5279.0
55	5018.5
56	4758.0
57	4573.0
58	4388.0
59	4155.5
60	3923.0
61	3923.0
62	3532.5
63	3142.0
64	2618.0
65	2094.0
66	1806.5
67	1519.0
68	1519.0
69	1308.5
70	1098.0
71	913.0
72	728.0
73	607.0
74	486.0
75	486.0
76	392.5
77	299.0
78	232.5
79	166.0
80	146.5
81	127.0
82	127.0
83	97.5
84	68.0
85	52.5
86	37.0
87	27.0
88	17.0
89	17.0
90	10.0
91	3.0
92	2.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	45425.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.54155200880572
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.17729243616509	60.438084755090806
2	5.7587923558696	7.8943313153549814
3	2.055564477276377	4.2267473858007705
4	1.0534767946041432	2.8882773802971933
5	0.6744820941063112	2.3115024766097965
6	0.44001927091697446	1.809576224545955
7	0.30833467159145656	1.4793615850302697
8	0.25052192066805845	1.3736929003852503
9	0.15737915529147262	0.9708310401761144
>10	1.0663240725871206	13.886626307099615
>50	0.051389111931909426	2.1860209135938358
>100	0.006423638991488678	0.53494771601541
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	137	0.30159603742432584	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	106	0.2333516785910842	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	86	0.18932305998899285	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	75	0.1651073197578426	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	66	0.14529444138690148	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	65	0.14309301045679693	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	64	0.14089157952669235	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	63	0.1386901485965878	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	63	0.1386901485965878	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	61	0.13428728673637863	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	58	0.12768299394606494	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	58	0.12768299394606494	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	58	0.12768299394606494	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	58	0.12768299394606494	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	57	0.1254815630159604	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	55	0.12107870115575124	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	54	0.11887727022564668	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	52	0.11447440836543754	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	49	0.10787011557512384	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	49	0.10787011557512384	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	49	0.10787011557512384	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	49	0.10787011557512384	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	47	0.10346725371491469	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	47	0.10346725371491469	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	46	0.10126582278481014	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.002201430930104568	0.0
21	0.0	0.0	0.0	0.002201430930104568	0.0
22	0.0	0.0	0.0	0.004402861860209136	0.0
23	0.0	0.0	0.0	0.006604292790313704	0.0
24	0.0	0.0	0.0	0.01100715465052284	0.0
25	0.0	0.0	0.0	0.01100715465052284	0.0
26	0.0	0.0	0.0	0.01100715465052284	0.0
27	0.0	0.0	0.0	0.05723720418271877	0.0
28	0.0	0.0	0.0	0.18712162905888827	0.0
29	0.0	0.0	0.0	0.39625756741882223	0.0
30	0.002201430930104568	0.0	0.0	0.631810676940011	0.0
31	0.002201430930104568	0.0	0.0	1.3847000550357733	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTTCGC	20	0.0018233794	37.0	23
CGGTTCG	20	0.0018233794	37.0	22
GGTATCA	60	3.274181E-11	33.916668	1
TTTCAGT	25	0.005441602	29.6	10
TCAGTTC	25	0.005441602	29.6	12
GAGTACT	25	0.005441602	29.6	13
AAGCTGC	25	0.005441602	29.6	6
GTTCGCC	25	0.005441602	29.6	24
GTATCAA	70	1.9463187E-10	29.07143	2
ACTTAGA	35	8.738317E-4	26.428572	1
CCCCCGG	35	8.738317E-4	26.428572	18
AACGCAG	80	9.1677066E-10	25.437502	7
ACGCAGA	80	9.1677066E-10	25.437502	8
TCAACGC	80	9.1677066E-10	25.437502	5
ATCAACG	80	9.1677066E-10	25.437502	4
CAACGCA	85	1.8517312E-9	23.941175	6
CGCAGAG	90	3.588866E-9	22.611113	9
TATCAAC	90	3.588866E-9	22.611113	3
AGAGTAC	100	4.947651E-10	22.2	12
GCAGAGT	95	6.701157E-9	21.421053	10
>>END_MODULE