FastQCFastQC Report
Fri 10 Feb 2017
ERR1632551.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632551.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041598
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA44930.43135643501619625No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA38280.3675122264059647No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA33310.31979708102358106No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA30710.29483543555191155No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA28610.2746741065171016No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA27320.26228929011000407No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA26960.2588330622754652No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG26460.2540327458386057No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT26370.25316868887997096No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC26070.2502884990178552No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT22470.21572622067246675No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA22080.2119819738517163No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC21960.21082989790687No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT21630.20766168905854274No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT21210.20362942325158076No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA21170.203245397936632No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA20580.19758102454113777No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC19270.18500419547656582No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT18530.17789972715001373No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA18060.17338742969936577No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18050.1732914233706286No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA17860.17146730312462197No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG17260.16570692340039056No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC17120.16436283479806987No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC16850.16177066392216577No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA16790.1611946259497426No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC16730.16061858797731945No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA16710.16042657531984508No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC16590.1592744993749988No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC16230.15581827154045993No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA16140.15495421458182523No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG16120.15476220192435086No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG15890.15255405636339547No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15660.1503459108024401No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA15630.15005789181622853No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG15560.1493858475150682No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA15090.14487355006442026No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT15060.14458553107820868No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC15030.1442975120919971No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA15030.1442975120919971No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT14760.14170534121609296No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC14680.14093729058619545No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC14650.14064927159998386No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT14390.13815310705281694No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC14000.1344088602320665No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC13930.13373681593090617No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT13610.13066461341131608No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT13530.12989656278141853No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA13070.12548027165950779No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC12890.12375215774223837No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG12800.12288810078360365No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC12670.12164001851002017No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA12460.11962388560653918No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC12380.11885583497664165No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC12340.1184718096616929No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12300.11808778434674413No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC12140.11655168308694909No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG12110.11626366410073753No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA11850.11376749955357057No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC11830.11357548689609619No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC11820.11347948056735901No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11780.11309545525241024No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC11410.10954322108913418No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT11160.10714306287070442No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT11100.10656702489828129No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC11080.10637501224080692No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA11080.10637501224080692No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG11070.10627900591206971No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC11030.10589498059712096No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA11010.10570296793964659No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT10810.10378284136490278No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG10710.10282277807753086No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG10680.1025347590913193No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTCCG200.001841856737.05
GACACTA250.00549610929.625
TGCCTTT250.00549610929.66
ATACACA2700.029.46296337
GGTATCA24750.028.0303021
CTACTGT803.274181E-1127.7534
ATACGGC2200.025.22727229
TTGTAAC451.3230956E-424.66666633
TTTGCCC551.902437E-523.5454543
TCTAGCC400.001931066323.12523
ATAACTG400.001931066323.12535
GATCTAG400.001931066323.12521
TAGGATC400.001931066323.12531
ATTGACT802.72521E-823.12531
TGTAACT400.001931066323.12534
TGATCTA400.001931066323.12520
ATTACCT1054.0017767E-1122.9047633
GCTTTTT903.8271537E-922.611111
TATTGAC855.1795723E-821.76470630
CTTTTTA957.1431714E-921.4210532
Produced by FastQC (version 0.11.5)