FastQCFastQC Report
Fri 10 Feb 2017
ERR1632549.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632549.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27995
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA690.24647258439006964No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT620.2214681193070191TruSeq Adapter, Index 7 (95% over 21bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA510.1821753884622254No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA470.16788712270048223No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT430.15359885693873906No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA380.13573852473656012No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA360.12859439185568852No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT340.12145025897481693No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA340.12145025897481693No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC320.11430612609394536No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA320.11430612609394536No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT310.11073405965350955No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA310.11073405965350955No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT280.10001786033220217No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC280.10001786033220217No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTAA358.653868E-426.42857223
AATACGG451.2786625E-424.66666628
AGCCGCG451.2786625E-424.66666619
CCAGCAG551.8180652E-523.54545414
TAATACG400.001884699623.12500227
TCTTATA400.001884699623.12500237
CGGTAAT400.001884699623.12500224
GTATCAA502.6118534E-422.1999991
CAGCAGC603.5617566E-521.58333415
AGCAGCC603.5617566E-521.58333416
TAGCCGG450.003734583320.5555555
CGCGGTA450.003734583320.55555522
GTAATAC450.003734583320.55555526
GGTAATA450.003734583320.55555525
CCGCGGT450.003734583320.55555521
CCGTGCC554.9726915E-420.1818189
GCCAGCA554.9726915E-420.18181813
AACTCCG554.9726915E-420.1818185
TAACTCC554.9726915E-420.1818184
CTAACTC554.9726915E-420.1818183