##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632549.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27995
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.008215752813	34.0	33.0	34.0	31.0	34.0
2	33.131059117699586	34.0	33.0	34.0	31.0	34.0
3	33.210216110019644	34.0	33.0	34.0	31.0	34.0
4	36.51662796928023	37.0	37.0	37.0	35.0	37.0
5	36.47015538489016	37.0	37.0	37.0	35.0	37.0
6	36.526236828005004	37.0	37.0	37.0	35.0	37.0
7	36.52280764422218	37.0	37.0	37.0	35.0	37.0
8	36.50230398285408	37.0	37.0	37.0	35.0	37.0
9	38.25290230398286	39.0	39.0	39.0	37.0	39.0
10	38.301018038935524	39.0	39.0	39.0	37.0	39.0
11	38.36270762636185	39.0	39.0	39.0	37.0	39.0
12	38.331952134309695	39.0	39.0	39.0	37.0	39.0
13	38.37310233970352	39.0	39.0	39.0	37.0	39.0
14	39.91523486336846	41.0	40.0	41.0	38.0	41.0
15	39.88840864440078	41.0	40.0	41.0	38.0	41.0
16	39.83493480978746	41.0	40.0	41.0	38.0	41.0
17	39.84086444007858	41.0	40.0	41.0	38.0	41.0
18	39.83386318985533	41.0	40.0	41.0	38.0	41.0
19	39.843150562600464	41.0	40.0	41.0	38.0	41.0
20	39.821289515985	41.0	40.0	41.0	38.0	41.0
21	39.7942846936953	41.0	40.0	41.0	38.0	41.0
22	39.751669941060904	41.0	40.0	41.0	38.0	41.0
23	39.731416324343634	41.0	40.0	41.0	38.0	41.0
24	39.6869083764958	41.0	40.0	41.0	37.0	41.0
25	39.64500803714949	41.0	40.0	41.0	37.0	41.0
26	39.573959635649224	41.0	40.0	41.0	37.0	41.0
27	39.4729773173781	41.0	39.0	41.0	37.0	41.0
28	39.41375245579568	41.0	39.0	41.0	37.0	41.0
29	39.38053223789962	41.0	39.0	41.0	36.0	41.0
30	39.354063225576	41.0	39.0	41.0	36.0	41.0
31	39.27958564029291	41.0	39.0	41.0	36.0	41.0
32	39.22954098946241	41.0	39.0	41.0	36.0	41.0
33	39.16024290051795	41.0	39.0	41.0	35.0	41.0
34	39.123629219503485	41.0	39.0	41.0	35.0	41.0
35	39.02932666547598	41.0	39.0	41.0	35.0	41.0
36	38.979710662618324	40.0	39.0	41.0	35.0	41.0
37	38.92184318628326	40.0	39.0	41.0	35.0	41.0
38	38.83697088765851	40.0	38.0	41.0	35.0	41.0
39	38.72677263797107	40.0	38.0	41.0	35.0	41.0
40	38.66915520628684	40.0	38.0	41.0	35.0	41.0
41	38.604215038399715	40.0	38.0	41.0	35.0	41.0
42	38.51023397035185	40.0	38.0	41.0	35.0	41.0
43	37.755456331487764	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	1.0
20	1.0
21	6.0
22	4.0
23	12.0
24	16.0
25	21.0
26	33.0
27	37.0
28	81.0
29	105.0
30	145.0
31	178.0
32	236.0
33	315.0
34	476.0
35	739.0
36	1216.0
37	2242.0
38	5633.0
39	16495.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.52562957671013	16.395784961600285	12.123593498839078	27.95499196285051
2	21.82532595106269	18.94624040007144	31.473477406679763	27.754956242186108
3	21.52884443650652	20.19289158778353	28.155027683514916	30.12323629219503
4	16.177888908733703	15.045543847115555	32.98803357742454	35.7885336667262
5	17.76745847472763	32.51294874084658	32.80943025540275	16.91016252902304
6	35.65993927487051	32.99160564386497	14.809787462046794	16.53866761921772
7	30.698338989105196	27.254866940525098	19.528487229862478	22.518306840507233
8	28.322914806215394	30.21253795320593	19.746383282729056	21.718163957849615
9	27.979996427933564	13.002321843186282	17.017324522236112	42.00035720664405
10	19.75352741560993	22.2253973923915	29.62671905697446	28.39435613502411
11	40.2893373816753	19.05697445972495	17.99607072691552	22.65761743168423
12	23.704232898731917	23.889980353634577	25.45811752098589	26.947669226647612
13	34.99910698338989	17.499553491694947	20.01786033220218	27.483479192712984
14	25.01160921593142	18.792641543132703	21.814609751741383	34.3811394891945
15	29.983925701018038	23.6828005000893	19.242721914627612	27.090551884265047
16	27.494195392034293	23.06483300589391	21.225218789069476	28.21575281300232
17	27.32273620289337	22.982675477763888	21.014466869083765	28.680121450258977
18	26.81193070191106	21.203786390426863	23.02911234148955	28.955170566172534
19	28.15859974995535	21.70030362564744	23.068405072334343	27.072691552062867
20	29.68387212002143	21.010894802643328	22.15038399714235	27.154849080192893
21	28.051437756742274	21.789605286658333	21.428826576174316	28.730130380425077
22	29.373102339703514	22.15752813002322	20.471512770137522	27.997856760135736
23	29.137345954634757	21.664582961243077	21.478835506340417	27.71923557778175
24	28.430076799428473	21.900339346311842	21.078764065011608	28.59081978924808
25	29.223075549205213	21.400250044650832	21.696731559207002	27.679942846936957
26	28.25861761028755	22.457581711019824	21.271655652795143	28.012145025897482
27	27.740667976424362	21.778889087337024	22.57188783711377	27.908555099124843
28	27.29415967136989	22.286122521878905	21.46811930701911	28.951598499732096
29	27.644222182532594	22.704054295409897	21.51812823718521	28.1335952848723
30	27.140560814431147	21.357385247365603	23.647079835684945	27.85497410251831
31	28.322914806215394	22.075370601893198	21.532416502946955	28.069298088944457
32	25.997499553491693	21.746740489373103	22.550455438471158	29.70530451866405
33	26.40114306126094	21.753884622253974	23.822111091266297	28.022861225218787
34	26.91909269512413	21.410966243972137	23.622075370601895	28.047865690301837
35	26.86193963207716	22.97910341132345	23.575638506876228	26.583318449723166
36	27.022682621896767	21.360957313806036	23.929273084479373	27.687086979817828
37	27.34774066797642	21.328808715842115	23.600642971959278	27.72280764422218
38	26.21539560635828	20.607251294874086	25.275942132523667	27.901410966243972
39	25.918914091802108	20.17145918914092	26.083229148062152	27.82639757099482
40	25.565279514198963	20.61082336131452	26.972673691730662	26.851223432755848
41	23.33631005536703	20.24647258439007	27.665654581175207	28.75156277906769
42	22.7147704947312	20.475084836577963	29.1016252902304	27.708519378460437
43	21.52884443650652	21.025183068405074	28.580103589926775	28.865868905161634
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.5
7	3.0
8	1.5
9	0.0
10	1.0
11	2.0
12	2.0
13	5.5
14	9.0
15	11.5
16	14.0
17	15.5
18	17.0
19	17.0
20	17.0
21	17.0
22	19.0
23	21.0
24	29.0
25	37.0
26	37.0
27	45.5
28	54.0
29	88.0
30	122.0
31	146.5
32	171.0
33	171.0
34	230.5
35	290.0
36	383.5
37	477.0
38	576.5
39	676.0
40	676.0
41	784.5
42	893.0
43	1062.5
44	1232.0
45	1455.5
46	1679.0
47	1679.0
48	1896.0
49	2113.0
50	2411.0
51	2709.0
52	2907.5
53	3106.0
54	3106.0
55	2946.5
56	2787.0
57	2749.5
58	2712.0
59	2551.5
60	2391.0
61	2391.0
62	2149.5
63	1908.0
64	1625.0
65	1342.0
66	1194.5
67	1047.0
68	1047.0
69	896.5
70	746.0
71	642.5
72	539.0
73	431.0
74	323.0
75	323.0
76	266.0
77	209.0
78	179.5
79	150.0
80	118.5
81	87.0
82	87.0
83	71.5
84	56.0
85	37.5
86	19.0
87	17.0
88	15.0
89	15.0
90	14.0
91	13.0
92	8.5
93	4.0
94	4.0
95	4.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	27995.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.84193606001071
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.62638411607485	66.32970173245224
2	5.837151584574265	8.737274513305948
3	2.0427644138984347	4.586533309519557
4	1.1072928598701794	3.3148776567244154
5	0.6777395952653684	2.5361671727094124
6	0.32455135547919056	1.4574031076978031
7	0.25773195876288657	1.3502411144847295
8	0.2434135166093929	1.4574031076978031
9	0.13841160748377243	0.9323093409537417
>10	0.7302405498281787	8.647972852295052
>50	0.014318442153493701	0.6501160921593142
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	69	0.24647258439006964	No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT	62	0.2214681193070191	TruSeq Adapter, Index 7 (95% over 21bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	51	0.1821753884622254	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	47	0.16788712270048223	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	43	0.15359885693873906	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	38	0.13573852473656012	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	36	0.12859439185568852	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	34	0.12145025897481693	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	34	0.12145025897481693	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	32	0.11430612609394536	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	32	0.11430612609394536	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	31	0.11073405965350955	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	31	0.11073405965350955	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	28	0.10001786033220217	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	28	0.10001786033220217	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.003572066440435792	0.0
18	0.0	0.0	0.0	0.003572066440435792	0.0
19	0.0	0.0	0.0	0.003572066440435792	0.0
20	0.0	0.0	0.0	0.003572066440435792	0.0
21	0.0	0.0	0.0	0.003572066440435792	0.0
22	0.0	0.0	0.0	0.003572066440435792	0.0
23	0.0	0.0	0.0	0.003572066440435792	0.0
24	0.0	0.0	0.0	0.003572066440435792	0.0
25	0.0	0.0	0.0	0.003572066440435792	0.0
26	0.0	0.0	0.0	0.010716199321307376	0.0
27	0.0	0.0	0.0	0.02143239864261475	0.0
28	0.0	0.0	0.0	0.10358992677263797	0.0
29	0.0	0.0	0.0	0.23218431862832647	0.0
30	0.0	0.0	0.0	0.3572066440435792	0.0
31	0.0	0.0	0.0	0.7608501518128237	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGGTAA	35	8.653868E-4	26.428572	23
AATACGG	45	1.2786625E-4	24.666666	28
AGCCGCG	45	1.2786625E-4	24.666666	19
CCAGCAG	55	1.8180652E-5	23.545454	14
TAATACG	40	0.0018846996	23.125002	27
TCTTATA	40	0.0018846996	23.125002	37
CGGTAAT	40	0.0018846996	23.125002	24
GTATCAA	50	2.6118534E-4	22.199999	1
CAGCAGC	60	3.5617566E-5	21.583334	15
AGCAGCC	60	3.5617566E-5	21.583334	16
TAGCCGG	45	0.0037345833	20.555555	5
CGCGGTA	45	0.0037345833	20.555555	22
GTAATAC	45	0.0037345833	20.555555	26
GGTAATA	45	0.0037345833	20.555555	25
CCGCGGT	45	0.0037345833	20.555555	21
CCGTGCC	55	4.9726915E-4	20.181818	9
GCCAGCA	55	4.9726915E-4	20.181818	13
AACTCCG	55	4.9726915E-4	20.181818	5
TAACTCC	55	4.9726915E-4	20.181818	4
CTAACTC	55	4.9726915E-4	20.181818	3
>>END_MODULE