FastQCFastQC Report
Fri 10 Feb 2017
ERR1632548.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632548.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481951
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA10630.22056184134901682No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG8370.17366910744038294No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8360.1734616174673359No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC8070.16744440824897136No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG7390.1533350900817718No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA6860.14233812151027803No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC6790.14088569169894866No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT6710.13922577191457222No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6520.13528346242667824No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA6390.13258609277706654No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6390.13258609277706654No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG6020.12490896377432562No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT5720.11868426458291403No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC5710.11847677460986698No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC5530.11474195509502004No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG5440.11287454533759657No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA5430.1126670553645495No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG5340.11079964560712603No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5330.11059215563407898No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5260.10913972582274961No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5120.10623486620009089No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA5120.10623486620009089No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG5090.10561239628094972No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5020.10415996646962035No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT4960.10291502663133803No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4880.10125510684696162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5350.026.2803731
ATACGGC451.3216554E-424.66666629
CTCTAGC451.3216554E-424.6666662
GGTACTT1250.022.1999992
GGGTACT1250.022.1999991
CCTCTAG603.7205085E-521.5833341
CTTATAC6700.020.70895637
TAATACT450.00382263420.5555554
AGTATTA450.00382263420.55555515
GATGCTA450.00382263420.55555514
CTAGCAT450.00382263420.5555554
ATGTACT951.6710692E-719.4736844
TAAGGTA951.6710692E-719.4736845
TACTTAG1457.2759576E-1219.137934
GTATCAA7550.018.6225172
CGAACTA609.2262076E-418.524
TAAAGAC609.2262076E-418.537
GTCTCAA500.007029118.51
TAGCATG609.2262076E-418.55
TAGCATC500.007029118.55
Produced by FastQC (version 0.11.5)