FastQCFastQC Report
Fri 10 Feb 2017
ERR1632546.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632546.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1113897
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA26140.23467160787756855No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA21670.19454222428106008No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA16120.14471715068807978No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA16090.14444782596595557No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA16000.1436398517995829No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA13340.11975972643790225No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA13230.11877220245678011No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT13090.11751535375353377No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG12800.11491188143966631No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA12660.11365503273641998No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA12520.11239818403317363No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT12240.10988448662668092No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT11970.10746056412756295No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC11440.10270249403670179No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG250.005496272429.5999985
GGTATCA14650.027.9078481
CTAATTA451.3231763E-424.66666616
ATACGGC1600.023.12500229
CTTACAC502.7020302E-422.1999993
CTCGTAT603.7261532E-521.58333429
AGTATTA806.9597263E-720.812515
TATTAGA1900.020.4473692
TATATCT555.142956E-420.1818185
TATCTAT555.142956E-420.1818187
ACACTAA555.142956E-420.1818186
CGTATGC656.901886E-519.92307731
AGACCGT656.901886E-519.9230776
ATACACA2600.019.92307737
GTATCAA21000.019.2928582
AACTCCG13050.018.9961685
GCGGTAA13250.018.56981323
CGTGCCA13750.018.56727210
ATTAGAG2200.018.53
TAGACAG801.6165968E-518.55
Produced by FastQC (version 0.11.5)