##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632546.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1113897
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.0579461117141	34.0	33.0	34.0	31.0	34.0
2	33.19504227051514	34.0	33.0	34.0	31.0	34.0
3	33.26716204460556	34.0	34.0	34.0	31.0	34.0
4	36.54737017875082	37.0	37.0	37.0	35.0	37.0
5	36.51951392274151	37.0	37.0	37.0	35.0	37.0
6	36.56405125429012	37.0	37.0	37.0	35.0	37.0
7	36.56109496659027	37.0	37.0	37.0	35.0	37.0
8	36.538852335539104	37.0	37.0	37.0	35.0	37.0
9	38.32525897816405	39.0	39.0	39.0	37.0	39.0
10	38.362915960811456	39.0	39.0	39.0	37.0	39.0
11	38.42691290128261	39.0	39.0	39.0	37.0	39.0
12	38.391706773606536	39.0	39.0	39.0	37.0	39.0
13	38.42502044623515	39.0	39.0	39.0	37.0	39.0
14	39.983274037007014	41.0	40.0	41.0	38.0	41.0
15	39.97751318120078	41.0	40.0	41.0	38.0	41.0
16	39.939046428888844	41.0	40.0	41.0	38.0	41.0
17	39.9349239651422	41.0	40.0	41.0	38.0	41.0
18	39.93205206585528	41.0	40.0	41.0	38.0	41.0
19	39.93906707711754	41.0	40.0	41.0	38.0	41.0
20	39.91240033863095	41.0	40.0	41.0	38.0	41.0
21	39.879304819027254	41.0	40.0	41.0	38.0	41.0
22	39.84986672915	41.0	40.0	41.0	38.0	41.0
23	39.818585560424346	41.0	40.0	41.0	38.0	41.0
24	39.79279502503373	41.0	40.0	41.0	38.0	41.0
25	39.754888468143825	41.0	40.0	41.0	38.0	41.0
26	39.666943173381384	41.0	40.0	41.0	37.0	41.0
27	39.5886289306821	41.0	40.0	41.0	37.0	41.0
28	39.53959567177217	41.0	40.0	41.0	37.0	41.0
29	39.509355891972056	41.0	40.0	41.0	37.0	41.0
30	39.455666008616596	41.0	40.0	41.0	37.0	41.0
31	39.39684548930467	41.0	39.0	41.0	36.0	41.0
32	39.34121557020084	41.0	39.0	41.0	36.0	41.0
33	39.282028769266816	41.0	39.0	41.0	36.0	41.0
34	39.24139036194549	41.0	39.0	41.0	35.0	41.0
35	39.154758474077944	41.0	39.0	41.0	35.0	41.0
36	39.10797676984497	41.0	39.0	41.0	35.0	41.0
37	39.04461812896525	41.0	39.0	41.0	35.0	41.0
38	38.96649869781497	41.0	39.0	41.0	35.0	41.0
39	38.87195853835678	40.0	39.0	41.0	35.0	41.0
40	38.794992714766266	40.0	38.0	41.0	35.0	41.0
41	38.7330139142129	40.0	38.0	41.0	35.0	41.0
42	38.660102325439425	40.0	38.0	41.0	35.0	41.0
43	37.92797628506047	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	1.0
13	0.0
14	2.0
15	1.0
16	3.0
17	2.0
18	15.0
19	35.0
20	69.0
21	133.0
22	232.0
23	407.0
24	640.0
25	947.0
26	1326.0
27	1861.0
28	2643.0
29	3525.0
30	4861.0
31	6142.0
32	8439.0
33	11315.0
34	17162.0
35	26811.0
36	42534.0
37	81362.0
38	212895.0
39	690532.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.13468839578525	16.47683762502278	12.550711600803306	27.837762378388664
2	21.416522353503062	19.25815403039958	32.37004857720238	26.955275038894978
3	21.636740201293296	19.746080651981288	28.691521747522437	29.925657399202976
4	16.005070486768524	15.673621528740988	33.52563118493003	34.79567679956046
5	17.757117579093936	32.454257440319886	32.38890130775108	17.3997236728351
6	34.48164417356362	33.0956991535124	15.320357268221388	17.10229940470259
7	30.40703045254633	27.41680783770851	19.6554079955328	22.520753714212354
8	27.030147311645514	30.74853420019984	20.05580408242414	22.165514405730512
9	28.013900746657903	13.199245531678422	17.677487236252546	41.10936648541113
10	19.696165803480934	23.150075814909275	29.34418532413679	27.809573057472996
11	39.32607772531931	19.476037730598073	18.948699924678852	22.24918461940377
12	23.142085848152927	23.52802817495693	26.346511391986873	26.983374584903274
13	34.57231683001211	17.627482612844815	21.080315325384664	26.71988523175841
14	24.270646208760773	19.928144164137258	22.75309117449818	33.04811845260379
15	29.618896540703492	24.145051113343513	19.83217478815366	26.403877557799333
16	26.068119404217803	23.735767310622077	22.321992069284683	27.874121215875437
17	27.00285573980359	23.444537511098424	21.78002095346338	27.77258579563461
18	26.552275479689776	21.502077840231188	23.552536724670233	28.393109955408804
19	28.345619029407565	22.39578704314672	23.166504622958854	26.09208930448686
20	29.397691168932138	21.038121118918536	22.778587248192604	26.785600463956722
21	27.670332176134778	22.195319674978926	22.01505166097045	28.11929648791585
22	28.03481830007622	22.83721026270831	21.453689165156202	27.674282272059266
23	27.884355555316155	22.08803866066611	22.51025005004951	27.517355733968223
24	28.17334098215544	21.90696267249126	22.37648543806115	27.54321090729215
25	28.26266701499331	22.063530110952808	22.677410927581278	26.99639194647261
26	28.146408509943022	22.67507677998953	22.252326741161884	26.926187968905563
27	27.5145727118396	21.78118802725925	23.537813639860776	27.166425621040364
28	26.516724616369373	22.906516491201607	22.24936416921852	28.327394723210492
29	27.301447081731972	23.32603463336377	22.3244159917838	27.048102293120458
30	26.87250257429547	22.3496427407561	23.780834314124196	26.99702037082423
31	28.04855386090455	22.130591966761738	22.69213401239073	27.128720159942976
32	25.32487294606234	22.22359877080197	23.063263479477904	29.388264803657787
33	26.20610343685278	21.801387381418568	24.258167496635686	27.734341685092968
34	26.30772863200098	21.735761924127633	24.121619862518706	27.834889581352673
35	26.338162325601022	22.886676236671793	24.069640191148732	26.705521246578456
36	26.238422403507684	21.670046691929326	24.786762151258152	27.304768753304838
37	26.600664154764758	21.04350761336102	24.646892845568306	27.708935386305917
38	25.779762401730146	20.509526464296073	26.07278769940129	27.6379234345725
39	25.13374216826152	19.586909741205876	27.262754096653463	28.016593993879148
40	24.884437250481866	20.617974552404757	27.85428096134562	26.64330723576776
41	23.391749865562076	19.872214396842796	28.942981263079083	27.793054474516044
42	22.103210620012444	20.774901090495803	29.44697759308087	27.674910696410887
43	21.40224814323048	21.292543206418546	29.06938433266271	28.235824317688262
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8.0
1	9.0
2	10.0
3	28.5
4	47.0
5	47.0
6	57.5
7	68.0
8	63.0
9	58.0
10	103.5
11	149.0
12	149.0
13	311.0
14	473.0
15	811.0
16	1149.0
17	1164.5
18	1180.0
19	1180.0
20	1409.5
21	1639.0
22	1438.0
23	1237.0
24	1441.0
25	1645.0
26	1645.0
27	2080.5
28	2516.0
29	3724.5
30	4933.0
31	6035.0
32	7137.0
33	7137.0
34	9712.0
35	12287.0
36	14292.0
37	16297.0
38	22696.5
39	29096.0
40	29096.0
41	33687.0
42	38278.0
43	44317.5
44	50357.0
45	64425.0
46	78493.0
47	78493.0
48	89343.5
49	100194.0
50	111850.0
51	123506.0
52	127304.0
53	131102.0
54	131102.0
55	120873.5
56	110645.0
57	104794.0
58	98943.0
59	91082.5
60	83222.0
61	83222.0
62	75822.5
63	68423.0
64	56899.5
65	45376.0
66	39582.0
67	33788.0
68	33788.0
69	29264.0
70	24740.0
71	21336.0
72	17932.0
73	14558.0
74	11184.0
75	11184.0
76	9143.5
77	7103.0
78	5755.5
79	4408.0
80	3550.5
81	2693.0
82	2693.0
83	2219.5
84	1746.0
85	1387.0
86	1028.0
87	735.5
88	443.0
89	443.0
90	362.0
91	281.0
92	168.5
93	56.0
94	39.5
95	23.0
96	23.0
97	12.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1113897.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.21231683100284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.686736426857	35.32611032603114
2	7.989886427255022	6.745432346220366
3	2.5150091974800235	3.184930952307388
4	1.2782512713099325	2.158317906166682
5	0.7604802416485354	1.6050816452092789
6	0.5089208738388692	1.2889637501038305
7	0.3882845230606376	1.1473272515607325
8	0.2946199479893918	0.9949272471409426
9	0.24740605524707251	0.9399224510998215
>10	1.8391815861929113	15.855077373795268
>50	0.28057969359208057	8.278967414774163
>100	0.19118326645990613	15.549297689800154
>500	0.014969606975824966	4.375649098002434
>1k	0.00449088209274749	2.5499945477878523
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2614	0.23467160787756855	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2167	0.19454222428106008	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1612	0.14471715068807978	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1609	0.14444782596595557	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1600	0.1436398517995829	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1334	0.11975972643790225	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1323	0.11877220245678011	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1309	0.11751535375353377	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1280	0.11491188143966631	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1266	0.11365503273641998	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1252	0.11239818403317363	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1224	0.10988448662668092	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1197	0.10746056412756295	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1144	0.10270249403670179	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	1.7954981474947864E-4
11	0.0	8.977490737473932E-5	0.0	8.977490737473932E-5	1.7954981474947864E-4
12	0.0	8.977490737473932E-5	0.0	8.977490737473932E-5	1.7954981474947864E-4
13	8.977490737473932E-5	8.977490737473932E-5	0.0	8.977490737473932E-5	1.7954981474947864E-4
14	8.977490737473932E-5	8.977490737473932E-5	0.0	8.977490737473932E-5	1.7954981474947864E-4
15	1.7954981474947864E-4	8.977490737473932E-5	0.0	1.7954981474947864E-4	1.7954981474947864E-4
16	1.7954981474947864E-4	8.977490737473932E-5	0.0	2.6932472212421794E-4	1.7954981474947864E-4
17	1.7954981474947864E-4	8.977490737473932E-5	0.0	8.079741663726539E-4	1.7954981474947864E-4
18	1.7954981474947864E-4	8.977490737473932E-5	0.0	9.875239811221325E-4	1.7954981474947864E-4
19	1.7954981474947864E-4	8.977490737473932E-5	0.0	0.0013466236106210899	1.7954981474947864E-4
20	1.7954981474947864E-4	8.977490737473932E-5	0.0	0.0017954981474947863	1.7954981474947864E-4
21	1.7954981474947864E-4	8.977490737473932E-5	0.0	0.0035909962949895726	1.7954981474947864E-4
22	1.7954981474947864E-4	8.977490737473932E-5	0.0	0.005117169720360141	1.7954981474947864E-4
23	1.7954981474947864E-4	8.977490737473932E-5	0.0	0.006194468608857012	1.7954981474947864E-4
24	1.7954981474947864E-4	8.977490737473932E-5	0.0	0.008438841293225495	1.7954981474947864E-4
25	1.7954981474947864E-4	8.977490737473932E-5	0.0	0.010054789625970803	1.7954981474947864E-4
26	2.6932472212421794E-4	8.977490737473932E-5	0.0	0.013286686291461418	2.6932472212421794E-4
27	2.6932472212421794E-4	8.977490737473932E-5	0.0	0.04398970461362226	2.6932472212421794E-4
28	3.590996294989573E-4	8.977490737473932E-5	0.0	0.16078685910815813	2.6932472212421794E-4
29	3.590996294989573E-4	8.977490737473932E-5	0.0	0.32345899127118577	2.6932472212421794E-4
30	4.488745368736966E-4	8.977490737473932E-5	0.0	0.5369437210083159	2.6932472212421794E-4
31	4.488745368736966E-4	8.977490737473932E-5	0.0	1.1250591392202332	2.6932472212421794E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACCG	25	0.0054962724	29.599998	5
GGTATCA	1465	0.0	27.907848	1
CTAATTA	45	1.3231763E-4	24.666666	16
ATACGGC	160	0.0	23.125002	29
CTTACAC	50	2.7020302E-4	22.199999	3
CTCGTAT	60	3.7261532E-5	21.583334	29
AGTATTA	80	6.9597263E-7	20.8125	15
TATTAGA	190	0.0	20.447369	2
TATATCT	55	5.142956E-4	20.181818	5
TATCTAT	55	5.142956E-4	20.181818	7
ACACTAA	55	5.142956E-4	20.181818	6
CGTATGC	65	6.901886E-5	19.923077	31
AGACCGT	65	6.901886E-5	19.923077	6
ATACACA	260	0.0	19.923077	37
GTATCAA	2100	0.0	19.292858	2
AACTCCG	1305	0.0	18.996168	5
GCGGTAA	1325	0.0	18.569813	23
CGTGCCA	1375	0.0	18.567272	10
ATTAGAG	220	0.0	18.5	3
TAGACAG	80	1.6165968E-5	18.5	5
>>END_MODULE