FastQCFastQC Report
Fri 10 Feb 2017
ERR1632545.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632545.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81757
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5320.6507088078085057No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3870.47335396357498444No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3050.3730567413187862No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2720.33269322504495025No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2550.3118998984796409No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2370.2898834350575486No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC2160.26419756106510756No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2110.25808187678119304No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2100.25685873992441016No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2030.24829678192692983No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT2000.24462737135658108No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1990.2434042344997982No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1940.23728855021588366No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1930.23606541335910075No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1900.23239600278875203No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1890.23117286593196912No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1890.23117286593196912No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1870.22872659221840333No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1870.22872659221840333No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1810.22138777107770588No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1810.22138777107770588No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1720.21037953936665973No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT1700.20793326565309392No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1550.18958621280135032No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1510.18469366537421872No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT1510.18469366537421872Illumina PCR Primer Index 11 (95% over 22bp)
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1480.18102425480387No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1470.1798011179470871No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1450.1773548442335213No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1440.1761317073767384No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC1420.17368543366317257No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC1400.17123915994960676No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1360.16634661252247512No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1330.16267720195212643No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA1310.1602309282385606No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC1300.15900779138177773No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG1260.1541152439546461No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC1260.1541152439546461No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG1230.15044583338429737No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1220.14922269652751446No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC1210.14799955967073156No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG1190.14555328595716574No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC1190.14555328595716574No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1170.14310701224359992No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA1160.14188387538681704No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT1150.1406607385300341No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1150.1406607385300341No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1140.13943760167325123No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG1140.13943760167325123No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA1100.1345450542461196No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT1100.1345450542461196No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC1090.1333219173893367No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1080.13209878053255378No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG1080.13209878053255378No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA1070.1308756436757709No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT1070.1308756436757709No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA1010.12353682253507345No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT1000.12231368567829054No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG990.12109054882150765No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT990.12109054882150765No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA990.12109054882150765No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC980.11986741196472472No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA940.11497486453759312No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC940.11497486453759312No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC920.1125285908240273No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA900.1100823171104615No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT900.1100823171104615No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG860.10518976968332987No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA860.10518976968332987No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG840.10274349596976406No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG840.10274349596976406No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC830.10152035911298114No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA830.10152035911298114No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA820.10029722225619825No Hit
GGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAA820.10029722225619825No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC820.10029722225619825No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATGC250.005466898429.632
AGACTGT250.005466898429.637
ACGGAGC250.005466898429.631
GGTATCA1400.029.0714281
ACTCCTA405.873927E-527.757
CAGTGGG405.873927E-527.7524
GACTCCT405.873927E-527.756
CGGAGCT358.7988225E-426.4285732
AGTGGGG508.956982E-625.90000225
GTCCAGA508.956982E-625.9000021
GAGGCAG451.3085836E-424.66666617
CCTACGG451.3085836E-424.66666610
CAGCAGT451.3085836E-424.66666621
TCCTACG451.3085836E-424.6666669
CTCCTAC451.3085836E-424.6666668
CCAGACT451.3085836E-424.6666663
GCAGCAG451.3085836E-424.66666620
AGACTCC451.3085836E-424.6666665
TTCTTCT1103.6379788E-1223.54545415
AAGGCGG400.001915966723.12524
Produced by FastQC (version 0.11.5)