FastQCFastQC Report
Fri 10 Feb 2017
ERR1632544.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632544.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193151
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5610.29044633473292913No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4050.20968050903179378No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3170.16412029966192254No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT2770.14341111358470834No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2530.13098560193837983No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC2510.12995014263451912No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT2430.12580830541907626No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2310.119595549595912No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC2180.1128650641208174No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC2070.10717003794958348No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2040.10561684899379241No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2010.10406366003800137No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2000.10354593038607102No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTAAA200.00183814837.02
GGTATCA2250.034.5333331
ACCGACC250.00548516829.68
CAAGGAC250.00548516829.636
TCCCGAG250.00548516829.628
GTACACT358.8425964E-426.428574
GAAACCG358.8425964E-426.428575
AAGTACC502.6908398E-422.236
TAAGTAC502.6908398E-422.235
GTATCAA3600.021.5833322
GTATTAG450.003814467520.5555551
TATTAGA450.003814467520.5555552
CCTATAC450.003814467520.5555553
CATCTAA555.1218335E-420.18181831
CTAAGTA555.1218335E-420.18181834
TCAATGA851.234368E-619.58823632
CAATGAG851.234368E-619.58823633
TGAGCAA851.234368E-619.58823636
CGTCAAT951.6581544E-719.47368430
GTAACGT1052.2304448E-819.3809526
Produced by FastQC (version 0.11.5)