##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632544.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	193151
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.049075593706476	34.0	33.0	34.0	31.0	34.0
2	33.18615487364808	34.0	33.0	34.0	31.0	34.0
3	33.259455037768376	34.0	34.0	34.0	31.0	34.0
4	36.53644557884764	37.0	37.0	37.0	35.0	37.0
5	36.51134604532206	37.0	37.0	37.0	35.0	37.0
6	36.55902376896832	37.0	37.0	37.0	35.0	37.0
7	36.55929298838733	37.0	37.0	37.0	35.0	37.0
8	36.53449373805986	37.0	37.0	37.0	35.0	37.0
9	38.330176908222064	39.0	39.0	39.0	37.0	39.0
10	38.355597434131845	39.0	39.0	39.0	37.0	39.0
11	38.432402627995714	39.0	39.0	39.0	37.0	39.0
12	38.38494752809978	39.0	39.0	39.0	37.0	39.0
13	38.42175810635202	39.0	39.0	39.0	37.0	39.0
14	39.973808056908844	41.0	40.0	41.0	38.0	41.0
15	39.96577289271089	41.0	40.0	41.0	38.0	41.0
16	39.93199621021895	41.0	40.0	41.0	38.0	41.0
17	39.933083442488	41.0	40.0	41.0	38.0	41.0
18	39.92723827471771	41.0	40.0	41.0	38.0	41.0
19	39.94567462762295	41.0	40.0	41.0	38.0	41.0
20	39.91110581876356	41.0	40.0	41.0	38.0	41.0
21	39.883671324507766	41.0	40.0	41.0	38.0	41.0
22	39.85391740141133	41.0	40.0	41.0	38.0	41.0
23	39.803878830552264	41.0	40.0	41.0	38.0	41.0
24	39.79643905545402	41.0	40.0	41.0	38.0	41.0
25	39.76051379490658	41.0	40.0	41.0	38.0	41.0
26	39.67572520980994	41.0	40.0	41.0	37.0	41.0
27	39.565241702088	41.0	40.0	41.0	37.0	41.0
28	39.534504092652895	41.0	40.0	41.0	37.0	41.0
29	39.492826855672504	41.0	40.0	41.0	37.0	41.0
30	39.45168805753012	41.0	39.0	41.0	37.0	41.0
31	39.3893534074377	41.0	39.0	41.0	36.0	41.0
32	39.32702911193833	41.0	39.0	41.0	36.0	41.0
33	39.265414106062096	41.0	39.0	41.0	36.0	41.0
34	39.225942397398924	41.0	39.0	41.0	35.0	41.0
35	39.14585997483834	41.0	39.0	41.0	35.0	41.0
36	39.1051353604175	41.0	39.0	41.0	35.0	41.0
37	39.04861999161278	41.0	39.0	41.0	35.0	41.0
38	38.96807678966197	40.0	39.0	41.0	35.0	41.0
39	38.87963303322271	40.0	39.0	41.0	35.0	41.0
40	38.79377274774658	40.0	38.0	41.0	35.0	41.0
41	38.74264694461846	40.0	38.0	41.0	35.0	41.0
42	38.67255152704361	40.0	38.0	41.0	35.0	41.0
43	37.93636067118472	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	2.0
19	4.0
20	12.0
21	16.0
22	34.0
23	80.0
24	116.0
25	152.0
26	208.0
27	307.0
28	446.0
29	594.0
30	793.0
31	1101.0
32	1372.0
33	2002.0
34	2841.0
35	4638.0
36	7516.0
37	14672.0
38	38225.0
39	118017.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.571609776806746	16.368022945778176	12.402731541643584	27.657635735771496
2	22.075474628658405	19.253848025637975	31.495565645531215	27.175111700172405
3	21.61365977913653	19.502875988216474	28.557967600478385	30.325496632168615
4	16.175427515260083	15.410223089707017	33.65398056442886	34.76036883060403
5	17.692375395416022	32.61645034196044	32.55587597268458	17.13529828993896
6	35.22425459873364	33.03218725246051	14.894564356384382	16.848993792421474
7	30.773850510740303	27.06276436570352	19.413826488084453	22.74955863547173
8	27.861103488980127	30.309964742610703	19.93155614001481	21.897375628394364
9	27.59084861067248	12.886808766198465	17.343943339666893	42.178399283462156
10	19.759152165921996	22.992891571878996	29.479008651262482	27.76894761093652
11	39.89987108531667	19.225890624433735	18.60720369037696	22.26703459987264
12	23.091260205745765	23.140962252331075	26.620105513303066	27.147672028620097
13	35.729558739017655	17.261106595358036	20.098265087936383	26.911069577687925
14	24.489130265957723	19.172564470284907	22.50053067289323	33.83777459086414
15	29.661767218393898	23.732209514835542	19.72032244202722	26.885700824743335
16	26.45494975433728	23.35012503171094	21.928957137162115	28.26596807678966
17	27.20876412754788	23.14355090059073	21.66646820363343	27.981216768227966
18	26.837034237461882	21.02862527245523	23.1891111099606	28.94522938012229
19	28.885690470150298	21.993673343653413	22.556963204953636	26.563672981242654
20	29.702667860896398	21.032767109670672	22.184715585215713	27.079849444217217
21	28.049039352630846	21.75551770376545	21.733255328732444	28.462187614871265
22	28.43526567297089	22.580778768942434	21.079880507996336	27.904075050090345
23	28.53829387370503	21.894786980134715	21.859063634151518	27.707855512008738
24	28.24888299827596	21.461447261469004	21.779333267754243	28.510336472500793
25	29.09847735709367	21.69753198274925	22.117928460116694	27.086062200040384
26	28.42232243167263	22.16452412879043	22.019042096598	27.394111342938942
27	27.567550776335615	21.94655994532775	23.26004007227506	27.225849206061582
28	27.32887740679572	22.99910432770216	21.735843976992093	27.936174288510024
29	27.336643351574676	23.31440168572775	22.026808041376956	27.322146921320623
30	27.359423456259613	22.146403590972866	23.241919534457498	27.25225341831003
31	28.15154982371305	22.186268774171502	22.205424771292925	27.456756630822515
32	26.01798592810806	22.145368131669006	22.81375711231109	29.022888827911842
33	26.694658583181035	21.842496285289748	23.804691666105793	27.658153465423425
34	26.721062795429486	21.500276985363783	23.601741642549094	28.176918576657638
35	26.095645375897615	22.668792809770594	24.111705349700493	27.123856464631295
36	26.633048754601322	21.475943691723057	24.55747058001253	27.33353697366309
37	26.711225932042804	20.626349332905345	24.57093155096272	28.091493184089135
38	25.364611107371953	20.187314588068404	26.434758297911998	28.01331600664765
39	25.133185952959085	19.48268453179119	27.20928185719981	28.174847658049917
40	24.58905208878028	19.87978317482177	28.46840037069443	27.06276436570352
41	23.28903293278316	19.558273060973022	29.0187469906964	28.133947015547424
42	21.873042334753638	20.15883945721223	30.205383352920773	27.762734855113358
43	20.971675010742892	20.441519847166205	29.69697283472516	28.889832307365737
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	2.5
4	4.0
5	4.0
6	5.0
7	6.0
8	7.5
9	9.0
10	11.5
11	14.0
12	14.0
13	38.5
14	63.0
15	99.5
16	136.0
17	126.5
18	117.0
19	117.0
20	168.5
21	220.0
22	217.5
23	215.0
24	298.0
25	381.0
26	381.0
27	484.5
28	588.0
29	756.0
30	924.0
31	1172.5
32	1421.0
33	1421.0
34	1900.5
35	2380.0
36	2724.5
37	3069.0
38	3788.5
39	4508.0
40	4508.0
41	5424.5
42	6341.0
43	7428.0
44	8515.0
45	10582.5
46	12650.0
47	12650.0
48	14337.0
49	16024.0
50	17393.5
51	18763.0
52	19644.5
53	20526.0
54	20526.0
55	19857.0
56	19188.0
57	18500.0
58	17812.0
59	16980.0
60	16148.0
61	16148.0
62	14930.5
63	13713.0
64	11511.0
65	9309.0
66	8085.0
67	6861.0
68	6861.0
69	5897.5
70	4934.0
71	4203.0
72	3472.0
73	2768.5
74	2065.0
75	2065.0
76	1661.0
77	1257.0
78	975.0
79	693.0
80	556.0
81	419.0
82	419.0
83	312.5
84	206.0
85	161.0
86	116.0
87	83.0
88	50.0
89	50.0
90	33.5
91	17.0
92	14.0
93	11.0
94	7.5
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	193151.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.384814107497135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.74415746219306	53.24773980728181
2	6.983087287837487	8.573110301206608
3	2.0480518951487654	3.7715785459864244
4	1.0781560267345067	2.6472962911990154
5	0.6286004317652956	1.9293260325902548
6	0.417088804532501	1.5361751239527468
7	0.3304222230228785	1.4198034716071748
8	0.23685748671532775	1.1631562233591493
9	0.1721674187851126	0.9511618497742571
>10	1.182277968643508	13.688245350071387
>50	0.11842681311092854	4.972045274327321
>100	0.059710998207190856	5.7576516415450305
>500	9.95183303453181E-4	0.3427100870988607
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	561	0.29044633473292913	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	405	0.20968050903179378	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	317	0.16412029966192254	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	277	0.14341111358470834	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	253	0.13098560193837983	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	251	0.12995014263451912	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	243	0.12580830541907626	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	231	0.119595549595912	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	218	0.1128650641208174	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	207	0.10717003794958348	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	204	0.10561684899379241	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	201	0.10406366003800137	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	200	0.10354593038607102	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	5.17729651930355E-4	0.0
17	0.0	0.0	0.0	0.00103545930386071	0.0
18	0.0	0.0	0.0	0.00103545930386071	0.0
19	0.0	0.0	0.0	0.00103545930386071	0.0
20	0.0	0.0	0.0	0.001553188955791065	0.0
21	0.0	0.0	0.0	0.002588648259651775	0.0
22	0.0	0.0	0.0	0.003624107563512485	0.0
23	0.0	0.0	0.0	0.004659566867373195	0.0
24	0.0	0.0	0.0	0.00621275582316426	0.0
25	0.0	0.0	0.0	0.00621275582316426	0.0
26	0.0	0.0	0.0	0.0103545930386071	0.0
27	0.0	0.0	0.0	0.040900642502498044	0.0
28	0.0	0.0	0.0	0.17188624444087786	0.0
29	0.0	0.0	0.0	0.35257389296457176	0.0
30	0.0	0.0	0.0	0.6057436927585154	0.0
31	0.0	0.0	0.0	1.22494835646722	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCTAAA	20	0.001838148	37.0	2
GGTATCA	225	0.0	34.533333	1
ACCGACC	25	0.005485168	29.6	8
CAAGGAC	25	0.005485168	29.6	36
TCCCGAG	25	0.005485168	29.6	28
GTACACT	35	8.8425964E-4	26.42857	4
GAAACCG	35	8.8425964E-4	26.42857	5
AAGTACC	50	2.6908398E-4	22.2	36
TAAGTAC	50	2.6908398E-4	22.2	35
GTATCAA	360	0.0	21.583332	2
GTATTAG	45	0.0038144675	20.555555	1
TATTAGA	45	0.0038144675	20.555555	2
CCTATAC	45	0.0038144675	20.555555	3
CATCTAA	55	5.1218335E-4	20.181818	31
CTAAGTA	55	5.1218335E-4	20.181818	34
TCAATGA	85	1.234368E-6	19.588236	32
CAATGAG	85	1.234368E-6	19.588236	33
TGAGCAA	85	1.234368E-6	19.588236	36
CGTCAAT	95	1.6581544E-7	19.473684	30
GTAACGT	105	2.2304448E-8	19.38095	26
>>END_MODULE