FastQCFastQC Report
Fri 10 Feb 2017
ERR1632543.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632543.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136706
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTT2980.21798604304127103No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT2750.20116161690050183No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2560.18726317791464894No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1750.12801193802759206No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG1630.11923397656284289No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT1620.1185024797741138No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT1570.1148449958304683No Hit
GTGTGGGGCTGCCTGCGGGCTGCGTCTAGTTGCAGTAGTTCTC1510.11045601509809373No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1500.10972451830936464No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT1500.10972451830936464RNA PCR Primer, Index 27 (95% over 23bp)
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG1500.10972451830936464No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1410.10314104721080275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1050.029.952381
ATCTAAG250.00547962829.632
CATCTAA250.00547962829.631
CATGTTT405.9025057E-527.75000230
CCAAAAA405.9025057E-527.75000220
TTAGCAT358.8293134E-426.4285724
TTTAACG358.8293134E-426.42857234
TATGGGC358.8293134E-426.4285724
ATCTTAC509.014822E-625.9000021
AAAACAT451.3148993E-424.66666623
ACCTCTA451.3148993E-424.66666631
ACATCAC451.3148993E-424.66666626
TCTTACC551.8868095E-523.5454542
ACTGCTC400.001922542523.12500213
CACATGT400.001922542523.12500228
TAGCATG400.001922542523.1250025
ATGTACT400.001922542523.1250029
AACATCT400.001922542523.12500229
ATGTTTA400.001922542523.12500231
TACCAAA652.6542493E-622.7692318
Produced by FastQC (version 0.11.5)