##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632543.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	136706
Sequences flagged as poor quality	0
Sequence length	43
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.03409506532266	34.0	33.0	34.0	31.0	34.0
2	33.181930566324816	34.0	33.0	34.0	31.0	34.0
3	33.25255658127661	34.0	34.0	34.0	31.0	34.0
4	36.53316606440098	37.0	37.0	37.0	35.0	37.0
5	36.509041300308695	37.0	37.0	37.0	35.0	37.0
6	36.54150512779249	37.0	37.0	37.0	35.0	37.0
7	36.54412388629614	37.0	37.0	37.0	35.0	37.0
8	36.51463725074247	37.0	37.0	37.0	35.0	37.0
9	38.30357848229046	39.0	39.0	39.0	37.0	39.0
10	38.34981639430603	39.0	39.0	39.0	37.0	39.0
11	38.41786022559361	39.0	39.0	39.0	37.0	39.0
12	38.378066800286746	39.0	39.0	39.0	37.0	39.0
13	38.392630901350344	39.0	39.0	39.0	37.0	39.0
14	39.9630447822334	41.0	40.0	41.0	38.0	41.0
15	39.952291779439086	41.0	40.0	41.0	38.0	41.0
16	39.9325998858865	41.0	40.0	41.0	38.0	41.0
17	39.91988647169839	41.0	40.0	41.0	38.0	41.0
18	39.890407151112605	41.0	40.0	41.0	38.0	41.0
19	39.88694717130192	41.0	40.0	41.0	38.0	41.0
20	39.8505259461911	41.0	40.0	41.0	38.0	41.0
21	39.828332333621056	41.0	40.0	41.0	38.0	41.0
22	39.79778502772373	41.0	40.0	41.0	38.0	41.0
23	39.75806475209574	41.0	40.0	41.0	38.0	41.0
24	39.74466373092622	41.0	40.0	41.0	38.0	41.0
25	39.67493745702456	41.0	40.0	41.0	37.0	41.0
26	39.58711395256975	41.0	40.0	41.0	37.0	41.0
27	39.51463725074247	41.0	40.0	41.0	37.0	41.0
28	39.45399616695683	41.0	40.0	41.0	37.0	41.0
29	39.416894649832486	41.0	39.0	41.0	36.0	41.0
30	39.35467353298319	41.0	39.0	41.0	36.0	41.0
31	39.32035170365602	41.0	39.0	41.0	36.0	41.0
32	39.268956739279915	41.0	39.0	41.0	36.0	41.0
33	39.19053296856027	41.0	39.0	41.0	35.0	41.0
34	39.134215030796014	41.0	39.0	41.0	35.0	41.0
35	39.05748101765833	41.0	39.0	41.0	35.0	41.0
36	39.02568285225228	41.0	39.0	41.0	35.0	41.0
37	38.96824572440127	41.0	39.0	41.0	35.0	41.0
38	38.89900223838017	40.0	39.0	41.0	35.0	41.0
39	38.80428071920764	40.0	38.0	41.0	35.0	41.0
40	38.69428554708645	40.0	38.0	41.0	35.0	41.0
41	38.64670168097962	40.0	38.0	41.0	35.0	41.0
42	38.584473249162436	40.0	38.0	41.0	35.0	41.0
43	37.81745497637265	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	2.0
19	9.0
20	7.0
21	13.0
22	34.0
23	44.0
24	96.0
25	134.0
26	171.0
27	253.0
28	360.0
29	463.0
30	601.0
31	790.0
32	1055.0
33	1444.0
34	2250.0
35	3812.0
36	5644.0
37	10174.0
38	26319.0
39	83028.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.14729419337849	15.264874987198807	11.63079894079265	28.95703187863005
2	21.849077582549413	17.689055345047038	31.897648969321025	28.564218103082528
3	21.909791816013925	18.91650695653446	27.65496759469226	31.518733632759353
4	16.70592366099513	14.299299226076398	31.537752549266308	37.457024563662166
5	18.360569397100345	32.173423258671896	31.85229616841982	17.61371117580794
6	38.026129065293404	31.389258701154304	14.098869105964626	16.48574312758767
7	32.3570289526429	25.991543897122295	18.708030371746666	22.943396778488143
8	29.097479262066038	30.016970725498517	18.62683422819774	22.258715784237708
9	28.761722236039382	12.610273140900913	16.20119087677205	42.42681374628766
10	20.525799891738476	22.128509355843928	28.74124032595497	28.60445042646263
11	40.572469386859396	18.363495384255263	17.96117215045426	23.102863078431085
12	24.868696326423127	22.161426711336738	24.92355858557781	28.046318376662327
13	34.51933346012611	16.71982209998098	20.21491375652861	28.5459306833643
14	25.050107530027944	18.303512647579478	21.662545901423496	34.98383392096909
15	30.58168624639738	22.326744985589514	19.07816774684359	28.013401021169514
16	27.629365207086742	22.48401679516627	20.02033561072667	29.86628238702032
17	28.22041461237985	22.42622854885667	20.83961201410326	28.51374482466022
18	27.22557897970828	20.139569587289515	22.74077216801018	29.894079264992023
19	29.59197109124691	20.97493892001814	21.39042909601627	28.04266089271868
20	29.18379588313607	20.257340570274895	22.071452606323057	28.487410940265974
21	29.733149971471622	20.699896127456	20.68307170131523	28.883882199757142
22	29.938700569104505	21.799335800915834	19.732125875967405	28.52983775401226
23	29.492487527979755	21.74520503854988	20.429973812414964	28.332333621055405
24	30.017702222287244	20.66039530086463	20.218571240472254	29.103331236375872
25	29.243047123023132	20.83741752373707	21.46577326525537	28.453762087984437
26	29.789475224203766	21.089052419059882	21.55062689274794	27.570845463988412
27	29.900662736090588	20.40802890875309	21.579886764297104	28.111421590859216
28	27.745673196494668	21.21999034424239	22.024636811844395	29.009699647418547
29	29.188916360657174	21.527950492297336	21.280704577706906	28.002428569338583
30	27.967316723479584	20.691118165991252	22.80075490468597	28.540810205843197
31	28.134829488098546	21.104413851623192	21.847614588971954	28.913142071306307
32	26.339736368557343	21.155618626834226	22.48401679516627	30.02062820944216
33	27.89124105745176	21.117580793820316	22.964610185361288	28.026567963366638
34	27.959270258803564	20.929586119116937	22.729799716179247	28.381343905900252
35	27.764692113001626	21.703509721592322	23.255745907275465	27.276052258130584
36	27.807118926747908	20.348046172077304	22.90316445510804	28.94167044606674
37	26.41215455064152	20.372917062894093	23.841674835047474	29.373253551416912
38	26.733281640893598	19.351747545828275	25.430485860167074	28.484484953111057
39	25.399762995040454	18.776791069887203	26.693049317513495	29.13039661755885
40	25.39756850467427	19.222272614223222	28.207247670182728	27.172911210919786
41	23.65733764428774	18.20476058110105	28.81951048234898	29.318391292262223
42	22.01439585680219	19.68750457185493	29.60733252381022	28.69076704753266
43	21.48113469781868	19.145465451406668	29.743390926513836	29.63000892426082
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.5
4	3.0
5	3.0
6	4.5
7	6.0
8	6.5
9	7.0
10	5.5
11	4.0
12	4.0
13	16.5
14	29.0
15	45.5
16	62.0
17	59.0
18	56.0
19	56.0
20	64.0
21	72.0
22	63.5
23	55.0
24	71.5
25	88.0
26	88.0
27	136.0
28	184.0
29	253.5
30	323.0
31	458.0
32	593.0
33	593.0
34	874.0
35	1155.0
36	1360.5
37	1566.0
38	2180.5
39	2795.0
40	2795.0
41	3271.0
42	3747.0
43	4533.5
44	5320.0
45	6229.5
46	7139.0
47	7139.0
48	8228.5
49	9318.0
50	10721.5
51	12125.0
52	13606.0
53	15087.0
54	15087.0
55	14689.0
56	14291.0
57	13787.5
58	13284.0
59	12524.0
60	11764.0
61	11764.0
62	11353.0
63	10942.0
64	9160.0
65	7378.0
66	6356.0
67	5334.0
68	5334.0
69	4531.5
70	3729.0
71	3362.0
72	2995.0
73	2965.5
74	2936.0
75	2936.0
76	2866.5
77	2797.0
78	1865.0
79	933.0
80	599.0
81	265.0
82	265.0
83	210.5
84	156.0
85	122.0
86	88.0
87	65.0
88	42.0
89	42.0
90	31.0
91	20.0
92	17.0
93	14.0
94	8.5
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	136706.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.26755226544555
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.02837424346583	46.14281743303147
2	6.5415773653065905	7.09990783140462
3	2.419561378678205	3.9391102073061903
4	1.3816436842034319	2.9991368337892994
5	0.8492053863884509	2.3042148844966572
6	0.5917478803563967	1.9267625415124428
7	0.44077803388733877	1.674396149400904
8	0.3329424292665831	1.4454376545286967
9	0.2655451763786108	1.2969438064166898
>10	1.9194737622494507	21.08393194153878
>50	0.18197258279752515	6.556405717378901
>100	0.0471780770215806	3.530934999195354
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTT	298	0.21798604304127103	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT	275	0.20116161690050183	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	256	0.18726317791464894	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	175	0.12801193802759206	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	163	0.11923397656284289	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	162	0.1185024797741138	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	157	0.1148449958304683	No Hit
GTGTGGGGCTGCCTGCGGGCTGCGTCTAGTTGCAGTAGTTCTC	151	0.11045601509809373	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	150	0.10972451830936464	No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT	150	0.10972451830936464	RNA PCR Primer, Index 27 (95% over 23bp)
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	150	0.10972451830936464	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	141	0.10314104721080275	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	7.314967887290974E-4	0.0	0.0	0.0	0.0
12	7.314967887290974E-4	0.0	0.0	0.0	0.0
13	7.314967887290974E-4	0.0	0.0	0.0	0.0
14	7.314967887290974E-4	0.0	0.0	7.314967887290974E-4	0.0
15	7.314967887290974E-4	0.0	0.0	7.314967887290974E-4	0.0
16	7.314967887290974E-4	0.0	0.0	7.314967887290974E-4	0.0
17	7.314967887290974E-4	0.0	0.0	7.314967887290974E-4	0.0
18	7.314967887290974E-4	0.0	0.0	7.314967887290974E-4	0.0
19	7.314967887290974E-4	0.0	0.0	7.314967887290974E-4	0.0
20	7.314967887290974E-4	0.0	0.0	7.314967887290974E-4	0.0
21	7.314967887290974E-4	0.0	0.0	0.0014629935774581949	0.0
22	7.314967887290974E-4	0.0	0.0	0.0021944903661872926	0.0
23	7.314967887290974E-4	0.0	0.0	0.005120477521103682	0.0
24	7.314967887290974E-4	0.0	0.0	0.0058519743098327795	0.0
25	7.314967887290974E-4	0.0	0.0	0.010972451830936461	0.0
26	7.314967887290974E-4	0.0	0.0	0.016824426140769243	0.0
27	7.314967887290974E-4	0.0	0.0	0.04535280090120404	0.0
28	7.314967887290974E-4	0.0	0.0	0.18653168112591986	0.0
29	7.314967887290974E-4	0.0	0.0	0.3672113879420069	0.0
30	7.314967887290974E-4	0.0	0.0	0.6232352639971911	0.0
31	7.314967887290974E-4	0.0	0.0	1.2771933931210042	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	105	0.0	29.95238	1
ATCTAAG	25	0.005479628	29.6	32
CATCTAA	25	0.005479628	29.6	31
CATGTTT	40	5.9025057E-5	27.750002	30
CCAAAAA	40	5.9025057E-5	27.750002	20
TTAGCAT	35	8.8293134E-4	26.428572	4
TTTAACG	35	8.8293134E-4	26.428572	34
TATGGGC	35	8.8293134E-4	26.428572	4
ATCTTAC	50	9.014822E-6	25.900002	1
AAAACAT	45	1.3148993E-4	24.666666	23
ACCTCTA	45	1.3148993E-4	24.666666	31
ACATCAC	45	1.3148993E-4	24.666666	26
TCTTACC	55	1.8868095E-5	23.545454	2
ACTGCTC	40	0.0019225425	23.125002	13
CACATGT	40	0.0019225425	23.125002	28
TAGCATG	40	0.0019225425	23.125002	5
ATGTACT	40	0.0019225425	23.125002	9
AACATCT	40	0.0019225425	23.125002	29
ATGTTTA	40	0.0019225425	23.125002	31
TACCAAA	65	2.6542493E-6	22.76923	18
>>END_MODULE