FastQCFastQC Report
Fri 10 Feb 2017
ERR1632542.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632542.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46458
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT1320.2841275991217874Illumina PCR Primer Index 10 (95% over 22bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1100.23677299926815618No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA880.18941839941452493No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA820.1765035085453528No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC780.167893581299238No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT740.15928365405312325No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT730.15713117224159456No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC710.15282620861853718No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT680.1463687631839511No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG660.1420637995608937No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA620.13345387231477893No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA620.13345387231477893No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA610.13130139050325026No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA600.12914890869172155No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG560.12053898144560678No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC530.11408153601102072No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG530.11408153601102072No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG500.10762409057643463No Hit
AGGCAGAGGCGTGCGGCAGCCATCCGGAGTCCAGAGTAAATGG490.10547160876490592No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA490.10547160876490592No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT480.10331912695337724No Hit
ACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTCTCCCT470.10116664514184856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA652.8339855E-928.4615381
TCTGGAC358.741387E-426.428573
TAATACG400.001903578323.12527
AATACGG400.001903578323.12528
CGGTAAT400.001903578323.12524
GCGGTAA450.00377163320.55555523
ACTCCAT450.00377163320.55555511
GTAATAC450.00377163320.55555526
GGTAATA450.00377163320.55555525
TCTTATA758.9560945E-619.73333437
TAGTGAC609.056237E-418.55
TGACCAT609.056237E-418.58
CGCGGTA500.00693621418.49999822
AGCCGCG500.00693621418.49999819
GACTCCA500.00693621418.49999810
TGCCTGT650.001549297617.07692335
GTATCAA1107.4192576E-716.8181822
CTCTTAT1352.0647349E-816.44444537
TAACTCC700.002542634315.8571424
CTAACTC700.002542634315.8571423
Produced by FastQC (version 0.11.5)