##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632542.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	46458
Sequences flagged as poor quality	0
Sequence length	43
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.99735244737182	34.0	33.0	34.0	31.0	34.0
2	33.137715786301605	34.0	33.0	34.0	31.0	34.0
3	33.20586336045461	34.0	33.0	34.0	31.0	34.0
4	36.504498686986096	37.0	37.0	37.0	35.0	37.0
5	36.46476387274527	37.0	37.0	37.0	35.0	37.0
6	36.51043953678592	37.0	37.0	37.0	35.0	37.0
7	36.506177622799086	37.0	37.0	37.0	35.0	37.0
8	36.496685178010246	37.0	37.0	37.0	35.0	37.0
9	38.27136338197942	39.0	39.0	39.0	37.0	39.0
10	38.30197167333936	39.0	39.0	39.0	37.0	39.0
11	38.37847087692109	39.0	39.0	39.0	37.0	39.0
12	38.333677730423176	39.0	39.0	39.0	37.0	39.0
13	38.36051917861294	39.0	39.0	39.0	37.0	39.0
14	39.89145034224461	41.0	40.0	41.0	38.0	41.0
15	39.902923070300055	41.0	40.0	41.0	38.0	41.0
16	39.85576219380946	41.0	40.0	41.0	38.0	41.0
17	39.86071290197598	41.0	40.0	41.0	38.0	41.0
18	39.86620173059538	41.0	40.0	41.0	38.0	41.0
19	39.850251840371946	41.0	40.0	41.0	38.0	41.0
20	39.83419432605795	41.0	40.0	41.0	38.0	41.0
21	39.803736708424815	41.0	40.0	41.0	38.0	41.0
22	39.76931852425847	41.0	40.0	41.0	38.0	41.0
23	39.73593353136166	41.0	40.0	41.0	38.0	41.0
24	39.70691807654225	41.0	40.0	41.0	38.0	41.0
25	39.668539325842694	41.0	40.0	41.0	37.0	41.0
26	39.58207413147359	41.0	40.0	41.0	37.0	41.0
27	39.49309053338499	41.0	39.0	41.0	37.0	41.0
28	39.467088553101725	41.0	39.0	41.0	37.0	41.0
29	39.406065693744885	41.0	39.0	41.0	37.0	41.0
30	39.38557406689913	41.0	39.0	41.0	36.0	41.0
31	39.29753756080761	41.0	39.0	41.0	36.0	41.0
32	39.27155710534246	41.0	39.0	41.0	36.0	41.0
33	39.18939687459641	41.0	39.0	41.0	35.0	41.0
34	39.16810882948039	41.0	39.0	41.0	35.0	41.0
35	39.07372250204486	41.0	39.0	41.0	35.0	41.0
36	39.01775797494511	40.0	39.0	41.0	35.0	41.0
37	38.96422575229239	40.0	39.0	41.0	35.0	41.0
38	38.9080459770115	40.0	39.0	41.0	35.0	41.0
39	38.815101812389685	40.0	38.0	41.0	35.0	41.0
40	38.72911016401911	40.0	38.0	41.0	35.0	41.0
41	38.66266305049722	40.0	38.0	41.0	35.0	41.0
42	38.57525076413104	40.0	38.0	41.0	35.0	41.0
43	37.82937276680012	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	1.0
21	4.0
22	9.0
23	23.0
24	30.0
25	40.0
26	60.0
27	83.0
28	99.0
29	161.0
30	222.0
31	286.0
32	409.0
33	554.0
34	762.0
35	1222.0
36	1793.0
37	3561.0
38	9585.0
39	27552.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.28516079039132	15.930517887123855	11.754703172758191	28.029618149726637
2	22.37504843084076	18.446769124800895	29.476085927073914	29.702096517284428
3	22.542942012139996	18.991347023117655	27.44414309699083	31.021567867751514
4	16.716173748331826	14.249429592319945	32.35180162727625	36.68259503207198
5	18.197081234663568	32.160230746050196	32.16453570967325	17.478152309612984
6	37.317577166472944	31.996642128374013	13.825390675448793	16.86039002970425
7	31.092599767531965	26.255973137026995	18.94829738688708	23.703129708553963
8	29.40505402729347	28.61078823883938	19.376641267381288	22.60751646648586
9	27.72181325067803	12.366008007232338	16.490163158121312	43.42201558396832
10	20.848938826466917	22.301864049248785	28.18890180377976	28.66029532050454
11	41.42020749924663	18.216453570967325	17.329631064617505	23.03370786516854
12	25.16035989495889	22.437470403375094	24.699728787291747	27.70244091437427
13	37.22286796676568	16.608549657755393	18.967669723190838	27.20091265228809
14	25.39928537603857	18.560850660811916	21.25575788884584	34.78410607430367
15	30.481294933057818	22.988505747126435	18.511343579146754	28.01885574066899
16	27.551767187567265	22.676395884454774	21.014679925954628	28.75715700202333
17	28.905678247018812	22.189934995049292	19.72534332084894	29.179043437082957
18	27.435533169744712	20.356020491626843	21.963924404838778	30.24452193378966
19	30.102458134228765	20.848938826466917	21.854147832450817	27.1944552068535
20	31.901932928666753	19.058073959275042	20.601403418141118	28.438589693917088
21	29.084334237375693	20.276378664600283	20.898445908132075	29.74084118989195
22	29.40074906367041	21.427956433768134	20.196736837573724	28.97455766498773
23	29.693486590038315	20.33234319170003	20.226871582935125	29.74729863532653
24	28.890610874338112	19.97072624736321	21.688406732963106	29.450256145335572
25	30.005596452709977	20.306513409961685	20.8101941538594	28.877695983468936
26	30.012053898144558	21.524818115286926	21.018984889577684	27.44414309699083
27	28.391235094063454	20.31727581901933	22.383658358086876	28.90783072883034
28	27.510870033148223	21.824013087089416	20.304360928150157	30.360755951612212
29	28.976710146799263	22.20715484954152	20.7951267811787	28.02100822248052
30	28.634465538766197	20.375392827930604	21.87782513237763	29.112316500925566
31	28.262086185371732	21.522665633475395	21.910112359550563	28.305135821602306
32	26.38942700934177	20.38400275517672	21.55064789702527	31.675922338456242
33	26.58960781781394	21.057729562185198	23.35873261870937	28.99393000129149
34	28.141547203926127	19.88677945671359	22.824917129450256	29.146756209910023
35	26.804855998966808	21.526970597098455	23.380257436824657	28.287915967110077
36	26.96413965301993	20.82310904472857	24.224030306943906	27.98872099530759
37	27.095441043523184	19.77269792070257	24.24986008868225	28.882000947091996
38	26.619742563175343	19.89108442033665	24.531835205992508	28.957337810495503
39	25.496147057557362	18.679237160445993	26.58960781781394	29.235007964182703
40	25.082870549743852	19.19583279521288	27.510870033148223	28.210426621895046
41	22.661328511774077	18.91816264152568	28.851866201730598	29.56864264496965
42	21.58508760600973	19.3249817039046	30.270351715528005	28.819578974557665
43	21.90795987773903	19.59404193034569	29.620302208446336	28.877695983468936
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	2.5
4	4.0
5	4.0
6	3.0
7	2.0
8	2.0
9	2.0
10	4.5
11	7.0
12	7.0
13	12.5
14	18.0
15	20.5
16	23.0
17	25.5
18	28.0
19	28.0
20	26.0
21	24.0
22	23.0
23	22.0
24	31.5
25	41.0
26	41.0
27	50.5
28	60.0
29	72.5
30	85.0
31	114.5
32	144.0
33	144.0
34	207.5
35	271.0
36	324.0
37	377.0
38	538.0
39	699.0
40	699.0
41	836.5
42	974.0
43	1207.5
44	1441.0
45	1933.0
46	2425.0
47	2425.0
48	3000.0
49	3575.0
50	3984.0
51	4393.0
52	4577.0
53	4761.0
54	4761.0
55	4712.5
56	4664.0
57	4592.5
58	4521.0
59	4666.5
60	4812.0
61	4812.0
62	4381.0
63	3950.0
64	3466.0
65	2982.0
66	2603.0
67	2224.0
68	2224.0
69	1952.5
70	1681.0
71	1375.5
72	1070.0
73	818.0
74	566.0
75	566.0
76	417.5
77	269.0
78	207.5
79	146.0
80	115.0
81	84.0
82	84.0
83	72.0
84	60.0
85	41.5
86	23.0
87	17.5
88	12.0
89	12.0
90	10.0
91	8.0
92	7.0
93	6.0
94	3.5
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	46458.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.03883077187998
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.58739851502325	53.097421326789785
2	6.349316494344598	7.878083430195016
3	2.5119700228991744	4.67519049464032
4	1.464159322739574	3.633389297860433
5	0.8465755325792798	2.626027810065005
6	0.5863576434667962	2.1826165568900944
7	0.4198181944348067	1.8231520943648025
8	0.29491360766081465	1.463687631839511
9	0.2532787454028173	1.414180550174351
>10	1.6272291999167303	18.517801024581342
>50	0.05204357782249671	2.1675491842093932
>100	0.006939143709666228	0.5209005983899436
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT	132	0.2841275991217874	Illumina PCR Primer Index 10 (95% over 22bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	110	0.23677299926815618	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	88	0.18941839941452493	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	82	0.1765035085453528	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	78	0.167893581299238	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	74	0.15928365405312325	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	73	0.15713117224159456	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	71	0.15282620861853718	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	68	0.1463687631839511	No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG	66	0.1420637995608937	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	62	0.13345387231477893	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	62	0.13345387231477893	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	61	0.13130139050325026	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	60	0.12914890869172155	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	56	0.12053898144560678	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	53	0.11408153601102072	No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG	53	0.11408153601102072	No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG	50	0.10762409057643463	No Hit
AGGCAGAGGCGTGCGGCAGCCATCCGGAGTCCAGAGTAAATGG	49	0.10547160876490592	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	49	0.10547160876490592	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	48	0.10331912695337724	No Hit
ACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTCTCCCT	47	0.10116664514184856	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0021524818115286927	0.0
21	0.0	0.0	0.0	0.0021524818115286927	0.0
22	0.0	0.0	0.0	0.004304963623057385	0.0
23	0.0	0.0	0.0	0.00860992724611477	0.0
24	0.0	0.0	0.0	0.015067372680700849	0.0
25	0.0	0.0	0.0	0.015067372680700849	0.0
26	0.0	0.0	0.0	0.015067372680700849	0.0
27	0.0	0.0	0.0	0.03228722717293039	0.0
28	0.0	0.0	0.0	0.1765035085453528	0.0
29	0.0	0.0	0.0	0.4348013259287959	0.0
30	0.0	0.0	0.0	0.686641697877653	0.0
31	0.0	0.0	0.0	1.336691204959318	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	65	2.8339855E-9	28.461538	1
TCTGGAC	35	8.741387E-4	26.42857	3
TAATACG	40	0.0019035783	23.125	27
AATACGG	40	0.0019035783	23.125	28
CGGTAAT	40	0.0019035783	23.125	24
GCGGTAA	45	0.003771633	20.555555	23
ACTCCAT	45	0.003771633	20.555555	11
GTAATAC	45	0.003771633	20.555555	26
GGTAATA	45	0.003771633	20.555555	25
TCTTATA	75	8.9560945E-6	19.733334	37
TAGTGAC	60	9.056237E-4	18.5	5
TGACCAT	60	9.056237E-4	18.5	8
CGCGGTA	50	0.006936214	18.499998	22
AGCCGCG	50	0.006936214	18.499998	19
GACTCCA	50	0.006936214	18.499998	10
TGCCTGT	65	0.0015492976	17.076923	35
GTATCAA	110	7.4192576E-7	16.818182	2
CTCTTAT	135	2.0647349E-8	16.444445	37
TAACTCC	70	0.0025426343	15.857142	4
CTAACTC	70	0.0025426343	15.857142	3
>>END_MODULE