FastQCFastQC Report
Fri 10 Feb 2017
ERR1632541.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632541.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29088
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1340.46067106710671063No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1140.3919141914191419No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1000.3437843784378438No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT930.31971947194719474No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA810.27846534653465344No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT800.27502750275027504No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC740.25440044004400436No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC730.25096259625962597No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA730.25096259625962597No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA720.24752475247524752No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG690.2372112211221122No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC680.23377337733773376No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC670.23033553355335531No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT660.22689768976897692No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT660.22689768976897692No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA630.2165841584158416No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA620.21314631463146316No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT600.20627062706270624RNA PCR Primer, Index 26 (100% over 22bp)
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA590.20283278327832785No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA560.1925192519251925No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC510.17533003300330033No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC500.1718921892189219No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT500.1718921892189219No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC470.16157865786578657No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA450.1547029702970297No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC430.14782728272827284No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG430.14782728272827284No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA430.14782728272827284No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT420.1443894389438944No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG420.1443894389438944No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA420.1443894389438944No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG410.14095159515951594No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA410.14095159515951594No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT390.13407590759075907No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC380.13063806380638066No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG360.12376237623762376No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG360.12376237623762376No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT360.12376237623762376No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC350.12032453245324534No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG350.12032453245324534No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG340.11688668866886688No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA340.11688668866886688No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC340.11688668866886688No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA340.11688668866886688No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA340.11688668866886688No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC330.11344884488448846No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG330.11344884488448846No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC330.11344884488448846No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA330.11344884488448846No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC330.11344884488448846No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT320.11001100110011No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA320.11001100110011No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC320.11001100110011No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC320.11001100110011No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG320.11001100110011No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC320.11001100110011No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC320.11001100110011No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC310.10657315731573158No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC310.10657315731573158No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT300.10313531353135312No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT300.10313531353135312No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA300.10313531353135312No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT300.10313531353135312No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC300.10313531353135312No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT300.10313531353135312No Hit
CACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATC300.10313531353135312No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCTA250.005409694329.630
TCTAAGT250.005409694329.633
GGTATCA604.026333E-827.7500021
TAATGAG358.662164E-426.428574
GCGGTAA658.751158E-825.61538323
CCGCGGT658.751158E-825.61538321
TCTTATA601.264094E-624.66666837
CAGCAGC701.7924503E-723.78571315
CCAGCAG701.7924503E-723.78571314
GCCAGCA701.7924503E-723.78571313
GCAGCCG701.7924503E-723.78571317
CGCGGTA701.7924503E-723.78571322
CGTGCCA701.7924503E-723.78571310
CGGTAAT701.7924503E-723.78571324
GTGCCAG701.7924503E-723.78571311
AACTCCG753.4883305E-722.25
TAACTCC753.4883305E-722.24
TAATACG753.4883305E-722.227
TCCGTGC753.4883305E-722.28
AATACGG753.4883305E-722.228
Produced by FastQC (version 0.11.5)