FastQCFastQC Report
Fri 10 Feb 2017
ERR1632540.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632540.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53959
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2810.5207657666005671No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT2340.4336625956744936TruSeq Adapter, Index 12 (95% over 22bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2240.4151300061157545No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1840.3409996478807984No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1770.32802683518968107No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1750.32432031727793326No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1540.28540187920458127No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1440.2668692896458422No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1410.2613095127782205No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1400.2594562538223466No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1400.2594562538223466No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1380.2557497359105988No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1350.2501899590429771No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1350.2501899590429771No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1350.2501899590429771No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1300.24092366426360753No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1300.24092366426360753No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1260.23351062844011194No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1250.23165736948423804No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1140.2112715209696251No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1130.20941826201375116No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1110.20571174410200338No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1100.20385848514612948No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1080.20015196723438167No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1080.20015196723438167No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC1020.18903241349913824No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA970.17976611871976872No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC950.1760596008080209No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA900.1667933060286514No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA900.1667933060286514No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT890.16494004707277748No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC860.15938027020515577No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC850.15752701124928187No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC820.15196723438166013No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT820.15196723438166013No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA810.15011397542578625No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC810.15011397542578625No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG810.15011397542578625No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG800.14826071646991235No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA790.14640745751403844No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG780.1445541985581645No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT770.14270093960229063No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA750.13899442169054282No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC740.13714116273466892No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC740.13714116273466892No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA740.13714116273466892No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC740.13714116273466892No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA730.13528790377879502No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA730.13528790377879502No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA710.1315813858670472No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG710.1315813858670472No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT700.1297281269111733No Hit
TGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTA700.1297281269111733No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC690.1278748679552994No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC690.1278748679552994No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC670.12416835004355159No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA670.12416835004355159No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG670.12416835004355159No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC670.12416835004355159No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC660.12231509108767769No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC640.11860857317592986No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT640.11860857317592986No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA640.11860857317592986No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC640.11860857317592986RNA PCR Primer, Index 12 (95% over 23bp)
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG630.11675531422005597No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC630.11675531422005597No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA620.11490205526418207No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG600.11119553735243425No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG600.11119553735243425No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA600.11119553735243425No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA590.10934227839656036No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT590.10934227839656036No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT580.10748901944068645No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG580.10748901944068645No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG570.10563576048481255No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG570.10563576048481255No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG560.10378250152893863No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC560.10378250152893863No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC550.10192924257306474No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG540.10007598361719083No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC200.001826432337.037
ACTGTGC250.00545060929.68
CAGCACT250.00545060929.64
CACTGTG250.00545060929.67
TTATACA405.8374448E-527.75000237
CACGAAT358.759848E-426.4285712
GGTATCA856.548362E-1126.1176451
CAATGAG400.0019075623.12500233
CCTGCAC400.0019075623.1250028
ATGGCGT400.0019075623.12500217
GAATGGC400.0019075623.12500215
ACCTGCA400.0019075623.1250027
GCACGAA400.0019075623.12500211
TGGCGTA400.0019075623.12500218
GACCTGC400.0019075623.1250026
ACGAATG400.0019075623.12500213
CTGCACG400.0019075623.1250029
AATGAGC400.0019075623.12500234
GCAGCCG1155.456968E-1222.5217417
CGCGGTA1155.456968E-1222.5217422
Produced by FastQC (version 0.11.5)