FastQCFastQC Report
Fri 10 Feb 2017
ERR1632539.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632539.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18832
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1140.6053525913338997No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT1060.5628717077315208RNA PCR Primer, Index 17 (95% over 21bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA840.4460492778249787No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA800.4248088360237893No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA720.3823279524214104No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT640.33984706881903143No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA640.33984706881903143No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA600.318606627017842No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA590.3132965165675446No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT580.30798640611724726No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA510.2708156329651657No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC490.260195412064571No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG490.260195412064571No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA490.260195412064571No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC480.2548853016142736No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA480.2548853016142736No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC470.2495751911639762No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC470.2495751911639762No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC470.2495751911639762No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA470.2495751911639762No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC460.24426508071367886No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT460.24426508071367886No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG450.23895497026338147No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA440.23364485981308408No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC430.22833474936278675No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT420.22302463891248936No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC410.21771452846219203No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC400.21240441801189466No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC390.20709430756159727No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC370.19647408666100255No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA370.19647408666100255No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT350.18585386576040783No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA350.18585386576040783No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT350.18585386576040783No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC340.18054375531011044No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG340.18054375531011044No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA330.17523364485981308No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC320.16992353440951571No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG320.16992353440951571No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA320.16992353440951571No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC310.16461342395921835No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA310.16461342395921835No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG310.16461342395921835No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG310.16461342395921835No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC300.159303313508921No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC300.159303313508921No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC300.159303313508921No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC290.15399320305862363No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG290.15399320305862363No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA280.14868309260832627No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT280.14868309260832627No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC280.14868309260832627No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC280.14868309260832627No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA270.1433729821580289No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG270.1433729821580289No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA270.1433729821580289No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG270.1433729821580289No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG260.13806287170773152No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT260.13806287170773152No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA260.13806287170773152No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT260.13806287170773152No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC260.13806287170773152RNA PCR Primer, Index 17 (95% over 22bp)
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA260.13806287170773152No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT250.13275276125743415No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT250.13275276125743415No Hit
TTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT250.13275276125743415No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT250.13275276125743415No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT240.1274426508071368No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG240.1274426508071368No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG240.1274426508071368No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC240.1274426508071368No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA230.12213254035683943No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA230.12213254035683943No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA230.12213254035683943No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT230.12213254035683943No Hit
GAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAAC230.12213254035683943No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT230.12213254035683943No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG220.11682242990654204No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC220.11682242990654204No Hit
TCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTG220.11682242990654204No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT220.11682242990654204No Hit
CACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATC220.11682242990654204No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG210.11151231945624468No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA210.11151231945624468No Hit
TGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTA210.11151231945624468No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA210.11151231945624468No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG210.11151231945624468No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG210.11151231945624468No Hit
CCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCATCGCCT210.11151231945624468No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG210.11151231945624468No Hit
TCCTTCATCGCCTCTGACTGCCAGGGCATCCACCGTGTACGCT200.10620220900594733No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA200.10620220900594733No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT200.10620220900594733No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA190.10089209855564997No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG190.10089209855564997No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC190.10089209855564997No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC190.10089209855564997No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC190.10089209855564997No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC190.10089209855564997No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG190.10089209855564997No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG190.10089209855564997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA453.7399004E-628.7777791
CTCTTAT601.2245437E-624.66666637
GTATCAA551.7769966E-523.5454542
TATCAAC551.7769966E-523.5454543
TCAACGC603.4818604E-521.5833325
ATCAACG603.4818604E-521.5833324
GACCATG450.00368965920.5555579
CAGAGTA656.4524815E-519.92307711
AGAGTAC656.4524815E-519.92307712
GCAGAGT656.4524815E-519.92307710
CGCAGAG656.4524815E-519.9230779
CAACGCA656.4524815E-519.9230776
AACGCAG701.1403168E-418.57
ACCATGA500.006786887518.510
ACGCAGA701.1403168E-418.58
GCGGCTG500.006786887518.511
AGTACGG751.9344394E-417.26666614
GAGTACG751.9344394E-417.26666613
TCTCTTA1106.8556983E-716.81818237
GTACGGG907.753385E-414.38888915
Produced by FastQC (version 0.11.5)