FastQCFastQC Report
Fri 10 Feb 2017
ERR1632538.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632538.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360662
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA16920.4691373086158231No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA13710.3801343085770056No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA11570.32079897521779394No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA11120.3083219191375859No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA10250.28419961071585026No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9990.276990644980619No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA9780.2711680188098552No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC9460.2622954455972628No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9270.2570273552522861No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT8950.24815478203969368No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8710.24150035213024937No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA8490.23540045804659207No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT8170.22652788483399972No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG8120.22514154526953215No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT7870.21820984744719435No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC7770.2154371683182592No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA7730.21432809666668518No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT7470.20711913093145384No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC7280.2018510405864771No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC7060.1957511465028198No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC7020.19464207485124577No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA6940.19242393154809767No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6770.18771037702890794No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC6750.18715584120312093No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC6590.18271955459682473No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6490.1799468754678896No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6230.1727379097326583No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA6090.1688561589521491No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5950.16497440817163994No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5940.1646971402587464No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC5850.1622017290427048No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC5820.16136992530402428No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC5730.15887451408798267No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5650.15665637078483455No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC5580.15471549539458No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA5580.15471549539458No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5550.15388369165589943No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA5000.13863395644675625No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT4960.13752488479518218No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC4940.13697034896939517No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA4870.13502947357914058No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4790.13281133027599248No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC4670.12948411532127033No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT4670.12948411532127033No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC4650.1289295794954833No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA4630.1283750436696963No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA4590.12726597201812223No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA4570.12671143619233521No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4530.12560236454076115No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT4530.12560236454076115No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4440.12310695332471955No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG4440.12310695332471955No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4430.12282968541182603No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG4290.11894793463131685No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG4280.11867066671842334No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC4250.1178388629797428No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT4200.11645252341527523No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG4150.11506618385080769No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA4000.11090716515740501No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT3960.10979809350583093No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC3960.10979809350583093No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT3870.10730268228978934TruSeq Adapter, Index 11 (95% over 23bp)
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA3830.10619361063821528No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC3820.10591634272532177No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT3810.10563907481242825No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC3800.10536180689953475No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3790.10508453898664123No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA3720.10314366359638663No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACAACT251.2319464E-437.034
GGTATCA7950.031.8805051
TTAAACC303.5946255E-430.8333324
CGAAAAG250.00549140529.623
TAAACCG250.00549140529.65
AGGTGTT250.00549140529.67
ACTACCT453.998237E-628.7777774
GAGGGCT405.929004E-527.7534
GCGAAAA358.8575477E-426.42857222
GCAGTCG809.731593E-1025.43759
CAGTCGG809.731593E-1025.437510
GCCTTAT601.3331592E-624.66666626
GGTGATT901.4188117E-1024.66666615
CTCACAA851.9645086E-923.94117532
TAGCGAA551.8979685E-523.54545410
GCACCGA551.8979685E-523.5454546
TCACTAC551.8979685E-523.5454542
GCTTAAA400.001928632323.1252
GTATAGA400.001928632323.1251
GTATCAA11050.023.1040732
Produced by FastQC (version 0.11.5)