##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632538.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	360662
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.02243374683221	34.0	33.0	34.0	31.0	34.0
2	33.15287443645297	34.0	33.0	34.0	31.0	34.0
3	33.21520980862969	34.0	33.0	34.0	31.0	34.0
4	36.50197692021893	37.0	37.0	37.0	35.0	37.0
5	36.48697672613139	37.0	37.0	37.0	35.0	37.0
6	36.5403230725721	37.0	37.0	37.0	35.0	37.0
7	36.55092025220289	37.0	37.0	37.0	35.0	37.0
8	36.52319623359267	37.0	37.0	37.0	35.0	37.0
9	38.324996811419	39.0	39.0	39.0	37.0	39.0
10	38.34859508348537	39.0	39.0	39.0	37.0	39.0
11	38.42332987672668	39.0	39.0	39.0	37.0	39.0
12	38.37472481159645	39.0	39.0	39.0	37.0	39.0
13	38.41431312419939	39.0	39.0	39.0	37.0	39.0
14	39.90363830955299	41.0	40.0	41.0	38.0	41.0
15	39.92558129217938	41.0	40.0	41.0	38.0	41.0
16	39.867845794677564	41.0	40.0	41.0	38.0	41.0
17	39.894050385125134	41.0	40.0	41.0	38.0	41.0
18	39.907855554508096	41.0	40.0	41.0	38.0	41.0
19	39.896886835874035	41.0	40.0	41.0	38.0	41.0
20	39.895539313817366	41.0	40.0	41.0	38.0	41.0
21	39.839514559338106	41.0	40.0	41.0	38.0	41.0
22	39.81078960356234	41.0	40.0	41.0	38.0	41.0
23	39.781008257038444	41.0	40.0	41.0	38.0	41.0
24	39.76402837005285	41.0	40.0	41.0	38.0	41.0
25	39.715939023240594	41.0	40.0	41.0	38.0	41.0
26	39.61521590852377	41.0	40.0	41.0	37.0	41.0
27	39.48966899756559	41.0	39.0	41.0	37.0	41.0
28	39.471463586405	41.0	39.0	41.0	37.0	41.0
29	39.43367751523587	41.0	39.0	41.0	37.0	41.0
30	39.39550604166782	41.0	39.0	41.0	36.0	41.0
31	39.3164874591723	41.0	39.0	41.0	36.0	41.0
32	39.26613006083258	41.0	39.0	41.0	36.0	41.0
33	39.191339814008685	41.0	39.0	41.0	35.0	41.0
34	39.1552922126534	41.0	39.0	41.0	35.0	41.0
35	39.062321508781075	41.0	39.0	41.0	35.0	41.0
36	39.007691411903664	40.0	39.0	41.0	35.0	41.0
37	38.945572308699006	40.0	39.0	41.0	35.0	41.0
38	38.86891327614221	40.0	39.0	41.0	35.0	41.0
39	38.762586576905804	40.0	38.0	41.0	35.0	41.0
40	38.67515291325396	40.0	38.0	41.0	35.0	41.0
41	38.61949137974059	40.0	38.0	41.0	35.0	41.0
42	38.539025458739765	40.0	38.0	41.0	35.0	41.0
43	37.81851428761555	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	1.0
18	4.0
19	6.0
20	19.0
21	38.0
22	68.0
23	138.0
24	215.0
25	326.0
26	422.0
27	610.0
28	901.0
29	1169.0
30	1575.0
31	2175.0
32	2793.0
33	3813.0
34	5991.0
35	9227.0
36	14413.0
37	28102.0
38	73819.0
39	214835.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.847874186911845	15.86721085115704	13.056268750242609	28.228646211688506
2	22.38134319667722	19.855432510217323	30.865464063305808	26.897760229799644
3	21.51210828975606	18.965402509829147	29.927189446074166	29.59529975434063
4	15.141323455201825	16.80409912882422	35.941685012560235	32.112892403413724
5	17.329799091670317	32.295334690097654	32.79913048782517	17.575735730406862
6	30.880713798514954	35.05664583460415	16.714541592959613	17.34809877392129
7	28.7673777664406	27.66080152608259	19.548496930644205	24.0233237768326
8	26.672618684530114	29.402321286966743	21.657396676112263	22.267663352390883
9	28.606285109049466	12.059213335477539	17.22665542807393	42.107846127399064
10	20.690840731765476	21.978195651330054	27.717086912399974	29.613876704504495
11	40.62917634793796	18.55449146292096	18.119180839678148	22.69715134946293
12	22.3652616577294	23.55973182647465	26.406718756065235	27.66828775973072
13	38.17064176431118	16.90142016624984	19.275388036444095	25.65255003299488
14	24.011123988665286	20.907109703822417	23.24336913786315	31.838397169649145
15	31.285524951339482	23.300486327919216	19.210507344827015	26.20348137591429
16	24.254842484098685	23.997260593020613	22.857134935202488	28.890761987678214
17	27.65276075660868	23.35455357093345	20.599065052597723	28.393620619860144
18	27.45784141384454	19.588146242187975	22.460641819764763	30.493370524202717
19	29.216551785328093	21.811834903593947	22.413783542485763	26.5578297685922
20	32.371028830317584	18.957639008268128	21.511831021843168	27.159501139571123
21	28.26829552323228	20.85526060411133	21.033821140014748	29.842622732641644
22	29.45167497546179	22.422378847785463	19.733989164369962	28.391957012382786
23	29.749737981822317	20.665332083779273	20.90378248886769	28.68114744553072
24	28.653697922154258	19.751179774969362	22.464246302632382	29.130876000243994
25	29.110912710515663	21.245931093378285	22.273485978561645	27.369670217544407
26	29.43448436486239	22.14511093489195	21.218758837914724	27.201645862330935
27	26.91300996500879	21.330220538897915	24.983779827095727	26.772989668997564
28	26.658755288885438	22.915638464823022	20.464312846931477	29.961293399360066
29	27.809417127393516	23.62544432183041	21.007203420376975	27.5579351303991
30	27.20109132650515	21.27837143918683	23.5533546644781	27.967182569829923
31	29.298900355457462	21.633274367690525	21.702591345913902	27.36523393093811
32	25.802829241783165	21.140846554391647	21.90749233354221	31.14883187028298
33	26.248121509890147	20.8480516383761	24.56094625993312	28.342880591800636
34	26.10782394596603	21.110069816060467	23.679511564844645	29.102594673128856
35	25.73988942555634	24.012787596142648	24.554846365849468	25.692476612451546
36	26.431950136138543	22.07108040214938	24.857900194642074	26.63906926707
37	27.449523376457734	22.03697644886348	23.665648169199972	26.847852005478813
38	26.065956491119106	20.8181067037836	25.177590098208295	27.938346706889
39	25.61151438188664	19.35246851622849	26.41753220466808	28.618484897216785
40	25.117977496936188	21.010530635331694	27.673001314249905	26.198490553482205
41	23.30381354287394	19.74313900549545	28.38973886907964	28.563308582550977
42	21.073747719471417	20.321242603878424	30.861027776699512	27.743981899950647
43	20.030943099078915	21.459704654219188	29.417293754262996	29.092058492438905
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	2.5
2	1.0
3	2.5
4	4.0
5	4.0
6	6.0
7	8.0
8	12.0
9	16.0
10	25.0
11	34.0
12	34.0
13	77.5
14	121.0
15	212.0
16	303.0
17	293.5
18	284.0
19	284.0
20	329.5
21	375.0
22	303.5
23	232.0
24	246.5
25	261.0
26	261.0
27	322.0
28	383.0
29	456.5
30	530.0
31	631.0
32	732.0
33	732.0
34	1205.0
35	1678.0
36	1690.0
37	1702.0
38	2658.0
39	3614.0
40	3614.0
41	5029.0
42	6444.0
43	8880.0
44	11316.0
45	19995.5
46	28675.0
47	28675.0
48	33052.5
49	37430.0
50	42015.5
51	46601.0
52	47422.0
53	48243.0
54	48243.0
55	43632.5
56	39022.0
57	38269.0
58	37516.0
59	34634.5
60	31753.0
61	31753.0
62	27813.0
63	23873.0
64	18690.5
65	13508.0
66	11574.5
67	9641.0
68	9641.0
69	7954.0
70	6267.0
71	5246.0
72	4225.0
73	3051.5
74	1878.0
75	1878.0
76	1568.0
77	1258.0
78	1092.5
79	927.0
80	835.0
81	743.0
82	743.0
83	626.0
84	509.0
85	378.5
86	248.0
87	211.5
88	175.0
89	175.0
90	126.5
91	78.0
92	54.5
93	31.0
94	23.0
95	15.0
96	15.0
97	9.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	360662.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.466842115863678
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.74741839253011	22.90970902688681
2	9.342173212118908	5.505686861211179
3	3.3981780037110254	3.0040072415086083
4	1.8564698792169285	2.1881721929496702
5	1.22048900580638	1.7981978419122018
6	0.8476554181441301	1.4986637025065694
7	0.6255009333538601	1.2902076072524495
8	0.47908119981350683	1.1293608060466518
9	0.37848336897439794	1.0037438709343884
>10	3.2400153096206132	19.562430399510017
>50	0.4581638563210373	9.338922369432586
>100	0.36951911020674966	22.135770048628046
>500	0.03187226826581129	6.769398002858661
>1k	0.004980041916533015	1.8657300283621292
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1692	0.4691373086158231	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1371	0.3801343085770056	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1157	0.32079897521779394	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1112	0.3083219191375859	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1025	0.28419961071585026	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	999	0.276990644980619	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	978	0.2711680188098552	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	946	0.2622954455972628	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	927	0.2570273552522861	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	895	0.24815478203969368	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	871	0.24150035213024937	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	849	0.23540045804659207	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	817	0.22652788483399972	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	812	0.22514154526953215	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	787	0.21820984744719435	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	777	0.2154371683182592	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	773	0.21432809666668518	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	747	0.20711913093145384	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	728	0.2018510405864771	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	706	0.1957511465028198	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	702	0.19464207485124577	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	694	0.19242393154809767	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	677	0.18771037702890794	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	675	0.18715584120312093	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	659	0.18271955459682473	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	649	0.1799468754678896	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	623	0.1727379097326583	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	609	0.1688561589521491	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	595	0.16497440817163994	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	594	0.1646971402587464	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	585	0.1622017290427048	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	582	0.16136992530402428	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	573	0.15887451408798267	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	565	0.15665637078483455	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	558	0.15471549539458	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	558	0.15471549539458	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	555	0.15388369165589943	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	500	0.13863395644675625	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	496	0.13752488479518218	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	494	0.13697034896939517	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	487	0.13502947357914058	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	479	0.13281133027599248	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	467	0.12948411532127033	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	467	0.12948411532127033	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	465	0.1289295794954833	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	463	0.1283750436696963	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	459	0.12726597201812223	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	457	0.12671143619233521	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	453	0.12560236454076115	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	453	0.12560236454076115	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	444	0.12310695332471955	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	444	0.12310695332471955	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	443	0.12282968541182603	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	429	0.11894793463131685	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	428	0.11867066671842334	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	425	0.1178388629797428	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	420	0.11645252341527523	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	415	0.11506618385080769	No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA	400	0.11090716515740501	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	396	0.10979809350583093	No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC	396	0.10979809350583093	No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT	387	0.10730268228978934	TruSeq Adapter, Index 11 (95% over 23bp)
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	383	0.10619361063821528	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	382	0.10591634272532177	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	381	0.10563907481242825	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC	380	0.10536180689953475	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	379	0.10508453898664123	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	372	0.10314366359638663	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	2.772679128935125E-4	0.0	0.0	0.0	0.0
10	2.772679128935125E-4	0.0	0.0	0.0	0.0
11	2.772679128935125E-4	0.0	0.0	0.0	0.0
12	2.772679128935125E-4	0.0	0.0	0.0	0.0
13	2.772679128935125E-4	0.0	2.772679128935125E-4	0.0	2.772679128935125E-4
14	2.772679128935125E-4	0.0	2.772679128935125E-4	0.0	2.772679128935125E-4
15	2.772679128935125E-4	0.0	2.772679128935125E-4	2.772679128935125E-4	2.772679128935125E-4
16	2.772679128935125E-4	0.0	5.54535825787025E-4	5.54535825787025E-4	2.772679128935125E-4
17	2.772679128935125E-4	0.0	5.54535825787025E-4	0.00110907165157405	2.772679128935125E-4
18	2.772679128935125E-4	0.0	5.54535825787025E-4	0.0013863395644675624	2.772679128935125E-4
19	2.772679128935125E-4	0.0	5.54535825787025E-4	0.0016636074773610748	2.772679128935125E-4
20	2.772679128935125E-4	0.0	5.54535825787025E-4	0.002772679128935125	2.772679128935125E-4
21	2.772679128935125E-4	0.0	5.54535825787025E-4	0.00554535825787025	2.772679128935125E-4
22	2.772679128935125E-4	0.0	5.54535825787025E-4	0.008595305299698887	2.772679128935125E-4
23	2.772679128935125E-4	0.0	5.54535825787025E-4	0.00998164486416645	2.772679128935125E-4
24	2.772679128935125E-4	0.0	5.54535825787025E-4	0.013863395644675624	2.772679128935125E-4
25	2.772679128935125E-4	0.0	5.54535825787025E-4	0.016081538947823725	2.772679128935125E-4
26	2.772679128935125E-4	0.0	5.54535825787025E-4	0.01913148598965236	2.772679128935125E-4
27	2.772679128935125E-4	0.0	5.54535825787025E-4	0.047967348930577657	2.772679128935125E-4
28	2.772679128935125E-4	0.0	5.54535825787025E-4	0.15637910287194104	2.772679128935125E-4
29	2.772679128935125E-4	0.0	5.54535825787025E-4	0.34824849859425167	2.772679128935125E-4
30	2.772679128935125E-4	0.0	5.54535825787025E-4	0.6158120345364912	2.772679128935125E-4
31	5.54535825787025E-4	0.0	5.54535825787025E-4	1.2091653681286079	2.772679128935125E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACAACT	25	1.2319464E-4	37.0	34
GGTATCA	795	0.0	31.880505	1
TTAAACC	30	3.5946255E-4	30.833332	4
CGAAAAG	25	0.005491405	29.6	23
TAAACCG	25	0.005491405	29.6	5
AGGTGTT	25	0.005491405	29.6	7
ACTACCT	45	3.998237E-6	28.777777	4
GAGGGCT	40	5.929004E-5	27.75	34
GCGAAAA	35	8.8575477E-4	26.428572	22
GCAGTCG	80	9.731593E-10	25.4375	9
CAGTCGG	80	9.731593E-10	25.4375	10
GCCTTAT	60	1.3331592E-6	24.666666	26
GGTGATT	90	1.4188117E-10	24.666666	15
CTCACAA	85	1.9645086E-9	23.941175	32
TAGCGAA	55	1.8979685E-5	23.545454	10
GCACCGA	55	1.8979685E-5	23.545454	6
TCACTAC	55	1.8979685E-5	23.545454	2
GCTTAAA	40	0.0019286323	23.125	2
GTATAGA	40	0.0019286323	23.125	1
GTATCAA	1105	0.0	23.104073	2
>>END_MODULE