FastQCFastQC Report
Fri 10 Feb 2017
ERR1632537.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632537.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50180
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1960.39059386209645275No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1550.30888800318852133No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1520.30290952570745316No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1490.296931048226385No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1440.2869669190912714No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1210.2411319250697489No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1190.23714627341570346No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT1180.23515344758868076TruSeq Adapter, Index 11 (95% over 21bp)
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1110.22120366679952175No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1060.21123953766440814No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1030.20526106018333998No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA970.19330410522120367No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT960.19131127939418094No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA960.19131127939418094No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT930.1853328019131128No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC920.1833399760860901No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT890.17736149860502193No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC880.1753686727779992No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA840.16739736946990832No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA800.15942606616181745No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC800.15942606616181745No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC800.15942606616181745No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA790.15743324033479475No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA780.15544041450777202No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG750.14946193702670385No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC740.14746911119968115No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT720.1434834595456357No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA710.141490633718613No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA700.13949780789159028No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA690.13750498206456754No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC680.13551215623754484No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC680.13551215623754484No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA680.13551215623754484No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC660.1315265045834994No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC630.12554802710243124No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC620.12355520127540853No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC610.1215623754483858No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC600.11956954962136308No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA590.11757672379434038No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA580.11558389796731765No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC570.11359107214029494No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC570.11359107214029494No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG570.11359107214029494No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG560.11159824631327223No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG550.1096054204862495No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG540.10761259465922679No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT540.10761259465922679No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC530.10561976883220407No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG520.10362694300518134No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT520.10362694300518134No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA520.10362694300518134No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT520.10362694300518134No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG510.10163411717815864No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG510.10163411717815864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTTCT355.48951E-737.015
GTCAATG401.5600581E-632.37531
TTCTGCG401.5600581E-632.37518
CTGCGGG401.5600581E-632.37520
CTTCTGC401.5600581E-632.37517
ATGAGCA303.551048E-430.83333235
GGTATCA1050.029.952381
AACGTCA453.9087263E-628.77777728
GGGTAAC453.9087263E-628.77777724
TCTGCGG453.9087263E-628.77777719
TCTTCTG453.9087263E-628.77777716
GTAACGT453.9087263E-628.77777726
TGCGGGT453.9087263E-628.77777721
GGTGCTT453.9087263E-628.77777710
GTGCTTC453.9087263E-628.77777711
TCAATGA405.829341E-527.7532
AATGAGC405.829341E-527.7534
TGAGCAA358.7511813E-426.4285736
CGTCAAT508.866909E-625.89999830
TAACGTC508.866909E-625.89999827
Produced by FastQC (version 0.11.5)